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AB315785

Human TRAF1 ELISA Kit- Intracellular

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Human TRAF1 ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human TRAF1 with a sensitivity of 6.736 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

EBI6, TRAF1, TNF receptor-associated factor 1, Epstein-Barr virus-induced protein 6

2 Images
Sandwich ELISA - Human TRAF1 ELISA Kit- Intracellular (AB315785)
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Supplier Data

Sandwich ELISA - Human TRAF1 ELISA Kit- Intracellular (AB315785)

Interpolated concentrations of human TRAF1 in Raji cell extract and spleen tissue extract

Interpolated concentration of native TRAF1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Raji cell extract 200 µg/mL and spleen tissue extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human TRAF1 ELISA Kit- Intracellular (AB315785)
  • sELISA

Supplier Data

Sandwich ELISA - Human TRAF1 ELISA Kit- Intracellular (AB315785)

Example of human TRAF1 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.736 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB300075

Anti-TRAF1 antibody [EPR26204-112]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody [EPR26204-112] (AB300075)

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Proteins & Peptides

AB95858

Recombinant Human TRAF1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human TRAF1 protein (His tag N-Terminus) (AB95858)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TRAF1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TRAF1 protein in Cell Lysate, Tissue extracts. Quantitate Human TRAF1 with 6.736 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.1" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.9" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "87 - 97 %", "average": "= 91" }, { "sample": "Tissue Extracts", "range": "91 - 93 %", "average": "= 92" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAF1 or TNF Receptor Associated Factor 1 is a protein involved in signal transduction from various receptors including TNFR2 (tumor necrosis factor receptor 2). TRAF1 has a molecular mass of approximately 50 kDa. This protein is found mostly in the cytoplasm and functions as an adapter molecule. It helps to mediate signal transduction by interacting with receptor-associated signaling complexes. It does not have an intrinsic catalytic domain. TRAF1 expression is most prevalent in immune cells such as lymphocytes.
Biological function summary

TRAF1 plays a role in immune response regulation functioning as part of a larger TRAF protein complex that includes TRAF2 and TRAF3. This complex is significant for propagating signaling cascades important for immune system development and homeostasis. TRAF1 impacts the transcriptional activity that modulates inflammatory responses. It achieves this by interacting with other proteins to transmit signals from the cell surface to the nucleus subsequently affecting gene expression.

Pathways

TRAF1 is involved in the NF-κB and JNK signaling pathways which are important for immune cell activation and apoptosis regulation. In the NF-κB pathway TRAF1 works closely with TRAF2 facilitating the recruitment and activation of IKK (IκB kinase) which leads to the release and nuclear translocation of NF-κB. In the JNK pathway TRAF1 indirectly influences MAP kinase signaling cascades affecting cytokine production and cell death. These roles in signal transduction highlight TRAF1's function in maintaining immune cell function and integrity.

TRAF1 has connections to autoimmune diseases such as rheumatoid arthritis and certain cancers. In rheumatoid arthritis elevated TRAF1 expression correlates with inflammatory cell survival potentially exacerbating the disease. TRAF1 interaction with TNFR2 is significant in cancer aiding in tumor cell survival and proliferation. Its involvement with proteins like TRAF2 and cytokines like TNF-alpha highlights its impact on disease progression and potential as a therapeutic target.
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Product protocols

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Target data

Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2.
See full target information TRAF1
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websiteProtocolBooklet
en
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Product promise

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For full details, please see our Terms & Conditions

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