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AB285340

Human TRAIL ELISA Kit

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Human TRAIL ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human TRAIL in in Biofluids, Cell/Tissue Extracts, Cell Culture Media samples.

View Alternative Names

CD253, APO2L, TRAIL, TNFSF10, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, TNF-related apoptosis-inducing ligand, Apo-2L, Protein TRAIL

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ELISA - Human TRAIL ELISA Kit (AB285340)
  • ELISA

Supplier Data

ELISA - Human TRAIL ELISA Kit (AB285340)

Standard curve for Human TRAIL ELISA Kit ab285340, A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids

Assay type

Sandwich (quantitative)

Sensitivity

< 9.375 pg/mL

Range

15.6 - 1000 pg/mL

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human TRAIL ELISA Kit (ab285340, K4262) is a sandwich ELISA assay for the quantitative measurement of human TNFSF10 in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human TNFSF10 captured from the samples.

This product is manufactured by BioVision, an Abcam company and was previously called K4262 TRAIL/TNFSF10 (Human) ELISA Kit. K4262-100 is the same size as the 96 test size of ab285340.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAIL also known as TNF-related apoptosis-inducing ligand plays an important role in inducing apoptosis. This protein has a mass of around 32 kDa and is expressed across various tissues including immune cells. TRAIL interacts with specific receptors on the cell surface to initiate a signaling cascade that results in programmed cell death. It is an attractive therapeutic target because of its selective ability to induce apoptosis in cancer cells while sparing most normal cells.
Biological function summary

TRAIL's function extends beyond inducing cell death. It is an important member of the tumor necrosis factor (TNF) superfamily which regulates immune responses and inflammation. TRAIL can form a homotrimeric complex allowing it to bind effectively to death receptors on target cells specifically DR4 and DR5 triggering a series of downstream signaling events. This interaction is significant in modulating immune surveillance and tumor suppression.

Pathways

TRAIL is integral to both intrinsic and extrinsic apoptosis pathways. Through these pathways TRAIL is closely related to FADD and caspase-8 which are important for apoptosis induction. The extrinsic pathway involves binding to TRAIL death receptors leading to DISC formation while the intrinsic pathway involves mitochondrial events mediated by members of the Bcl-2 protein family.

TRAIL has a significant connection with cancer and autoimmune diseases. TRAIL plays a dual role in cancer where it acts as a tumor suppressor but can also promote tumor progression if cancer cells become resistant to its effects. In cancer TRAIL’s interaction with proteins like FLIP can alter the apoptotic potential of the cells. In autoimmune diseases TRAIL's dysregulation can contribute to tissue damage due to increased immune activity highlighting its complex role in maintaining immune balance.
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Product protocols

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Target data

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed : 10549288, PubMed : 26457518). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
See full target information TNFSF10
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websiteProtocolBooklet
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