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AB46074

Human TRAIL ELISA Kit (CD253)

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(6 Publications)

Human TRAIL ELISA Kit (CD253) is a Sandwich (quantitative) ELISA for the measurement of Human TRAIL (CD253) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD253, APO2L, TRAIL, TNFSF10, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, TNF-related apoptosis-inducing ligand, Apo-2L, Protein TRAIL

3 Images
ELISA - Human TRAIL ELISA Kit (CD253) (AB46074)
  • ELISA

Lab

ELISA - Human TRAIL ELISA Kit (CD253) (AB46074)

TRAIL measured in undiluted cell culture supernatants from control (-) or stimulated ((P/I); 48 hours with 50 ng x mL-1 PMA (ab120297) and 1 uM ionomycin (ab120116)) Jurkat cells (duplicates +/- SD).

ELISA - Human TRAIL ELISA Kit (CD253) (AB46074)
  • ELISA

Lab

ELISA - Human TRAIL ELISA Kit (CD253) (AB46074)

TRAIL measured in undiluted biological fluids (duplicates +/- SD).

Sandwich ELISA - Human TRAIL ELISA Kit (CD253) (AB46074)
  • sELISA

Supplier Data

Sandwich ELISA - Human TRAIL ELISA Kit (CD253) (AB46074)

Representative Standard Curve using ab46074

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 64 pg/mL

Range

93.75 - 3000 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TRAIL (CD253) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab46074) is designed for the quantitative measurement of TRAIL in cell culture supernatants, buffered solutions or Human serum, plasma, and other body fluids.

A monoclonal antibody specific for TRAIL has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TRAIL concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TRAIL are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TRAIL present in the samples.

This kit will recognize both endogenous and recombinant Human TRAIL.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 12, "mean": "1619 pg/mL", "standardDeviation": "62.8", "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 12, "mean": "162.8 pg/mL", "standardDeviation": "13.1", "coefficientOfVariability": "8" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 33, "mean": "116.3 pg/mL", "standardDeviation": "8.7", "coefficientOfVariability": "7.4" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 40, "mean": "1500 pg/mL", "standardDeviation": "82.9", "coefficientOfVariability": "5.5" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46074-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46074-CMP03" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46074-CMP10" }, { "size":"4 x 1 Vial", "name":"Control", "number":"AB46074-CMP06" }, { "size":"4 x 1 Vial", "name":"TRAIL Standard (Lyophilized)", "number":"AB46074-CMP08" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46074-CMP01" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46074-CMP09" }, { "size":"2 x 1 Unit", "name":"TRAIL Microplate (12 x 8 well strips)", "number":"AB46074-CMP07" }, { "size":"4 x 1 Unit", "name":"Plastic Plate Covers", "number":"AB46074-CMP11" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46074-CMP04" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46074-CMP02" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46074-CMP12" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-TRAIL", "number":"AB46074-CMP05" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46074-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46074-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46074-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Control", "number":"AB46074-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"TRAIL Standard (Lyophilized)", "number":"AB46074-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46074-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46074-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TRAIL Microplate (12 x 8 well strips)", "number":"AB46074-CMP07", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Plastic Plate Covers", "number":"AB46074-CMP11", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46074-CMP12", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46074-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46074-CMP02", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-TRAIL", "number":"AB46074-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAIL also known as TNF-related apoptosis-inducing ligand plays an important role in inducing apoptosis. This protein has a mass of around 32 kDa and is expressed across various tissues including immune cells. TRAIL interacts with specific receptors on the cell surface to initiate a signaling cascade that results in programmed cell death. It is an attractive therapeutic target because of its selective ability to induce apoptosis in cancer cells while sparing most normal cells.
Biological function summary

TRAIL's function extends beyond inducing cell death. It is an important member of the tumor necrosis factor (TNF) superfamily which regulates immune responses and inflammation. TRAIL can form a homotrimeric complex allowing it to bind effectively to death receptors on target cells specifically DR4 and DR5 triggering a series of downstream signaling events. This interaction is significant in modulating immune surveillance and tumor suppression.

Pathways

TRAIL is integral to both intrinsic and extrinsic apoptosis pathways. Through these pathways TRAIL is closely related to FADD and caspase-8 which are important for apoptosis induction. The extrinsic pathway involves binding to TRAIL death receptors leading to DISC formation while the intrinsic pathway involves mitochondrial events mediated by members of the Bcl-2 protein family.

TRAIL has a significant connection with cancer and autoimmune diseases. TRAIL plays a dual role in cancer where it acts as a tumor suppressor but can also promote tumor progression if cancer cells become resistant to its effects. In cancer TRAIL’s interaction with proteins like FLIP can alter the apoptotic potential of the cells. In autoimmune diseases TRAIL's dysregulation can contribute to tissue damage due to increased immune activity highlighting its complex role in maintaining immune balance.
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Product protocols

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Target data

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed : 10549288, PubMed : 26457518). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
See full target information TNFSF10
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 14: PubMed36297678

2022

Injectable pH Thermo-Responsive Hydrogel Scaffold for Tumoricidal Neural Stem Cell Therapy for Glioblastoma Multiforme.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmine L King,Panita Maturavongsadit,Shawn D Hingtgen,S Rahima Benhabbour

Science advances 7: PubMed33658202

2021

Anti-EGFR VHH-armed death receptor ligand-engineered allogeneic stem cells have therapeutic efficacy in diverse brain metastatic breast cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Yohei Kitamura,Nobuhiko Kanaya,Susana Moleirinho,Wanlu Du,Clemens Reinshagen,Nada Attia,Agnieszka Bronisz,Esther Revai Lechtich,Hikaru Sasaki,Joana Liliana Mora,Priscilla Kaliopi Brastianos,Jefferey L Falcone,Aldebaran M Hofer,Arnaldo Franco,Khalid Shah

Frontiers in immunology 10:2674 PubMed31798594

2019

Macrophage Coordination of the Interferon Lambda Immune Response.

Applications

Unspecified application

Species

Unspecified reactive species

Scott A Read,Ratna Wijaya,Mehdi Ramezani-Moghadam,Enoch Tay,Steve Schibeci,Christopher Liddle,Vincent W T Lam,Lawrence Yuen,Mark W Douglas,David Booth,Jacob George,Golo Ahlenstiel

Cell death & disease 8:e2897 PubMed28661478

2017

KDM2B, an H3K36-specific demethylase, regulates apoptotic response of GBM cells to TRAIL.

Applications

Unspecified application

Species

Unspecified reactive species

Ibrahim Cagri Kurt,Ilknur Sur,Ezgi Kaya,Ahmet Cingoz,Selena Kazancioglu,Zeynep Kahya,Omer Duhan Toparlak,Filiz Senbabaoglu,Zeynep Kaya,Ezgi Ozyerli,Sercin Karahüseyinoglu,Nathan A Lack,Zeynep H Gümüs,Tamer T Onder,Tugba Bagci-Onder

Cell death and differentiation 23:1886-1896 PubMed27612013

2016

Silencing the epigenetic silencer KDM4A for TRAIL and DR5 simultaneous induction and antitumor therapy.

Applications

Unspecified application

Species

Human

Junjian Wang,Haibin Wang,Ling-Yu Wang,Demin Cai,Zhijian Duan,Yanhong Zhang,Peng Chen,June X Zou,Jianzhen Xu,Xinbin Chen,Hsing-Jien Kung,Hong-Wu Chen

PLoS pathogens 11:e1005146 PubMed26360709

2015

Simian Immunodeficiency Virus Infection of Chimpanzees (Pan troglodytes) Shares Features of Both Pathogenic and Non-pathogenic Lentiviral Infections.

Applications

sELISA

Species

Unspecified reactive species

Edward J D Greenwood,Fabian Schmidt,Ivanela Kondova,Henk Niphuis,Vida L Hodara,Leah Clissold,Kirsten McLay,Bernadette Guerra,Sharon Redrobe,Luis D Giavedoni,Robert E Lanford,Krishna K Murthy,François Rouet,Jonathan L Heeney
View all publications
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