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AB233614

Human TRAIL R2 ELISA Kit

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Human TRAIL R2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TRAIL R2 with a sensitivity of 10.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD262, DR5, KILLER, TRAILR2, TRICK2, ZTNFR9, UNQ160/PRO186, TNFRSF10B, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2

5 Images
Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)
  • sELISA

Supplier Data

Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)

Interpolated concentrations of native TRAIL R2 in human pancreas and colon cancer extract samples.

The concentrations of TRAIL R2 were measured in duplicate, interpolated from the TRAIL R2 standard curve and corrected for sample dilution. Undiluted samples are both 500 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)
  • sELISA

Supplier Data

Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)

Interpolated concentrations of spiked TRAIL R2 in human serum and plasma samples.

The concentrations of TRAIL R2 were measured in duplicate and interpolated from the TRAIL R2 standard curve and corrected for sample dilution. Undiluted samples are follows : serum 50% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)
  • sELISA

Supplier Data

Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)

Interpolated concentrations of native TRAIL R2 in human A549, MDA-MB-231, HepG2, and HT1080 cell extract samples.

The concentrations of TRAIL R2 were measured in duplicates, interpolated from the TRAIL R2 standard curves and corrected for sample dilution. Undiluted samples are as follows : A549 extract 250 μg/mL, MDA-MB-231 extract 62.5 μg/mL, HepG2 extract 62.5 μg/mL, and HT1080 extract 31.25 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)
  • sELISA

Supplier Data

Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)

Example of human TRAIL R2 standard curve in Sample Diluent NS.

The TRAIL R2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)
  • sELISA

Supplier Data

Sandwich ELISA - Human TRAIL R2 ELISA Kit (AB233614)

Example of human TRAIL R2 standard curve in 1X Cell Extraction Buffer PTR.

The TRAIL R2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10.1 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TRAIL R2 ELISA Kit (ab233614) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TRAIL R2 protein in edta plasma, serum, tissue extracts, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TRAIL R2 with 10.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "HepG2", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "HepG2", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]
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Recovery

[ { "sample": "Serum", "range": "90 - 93 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "80 - 82 %", "average": "= 81" }, { "sample": "Cell culture extracts", "range": "99 - 100 %", "average": "= 100" }, { "sample": "Tissue Extracts", "range": "104 - 105 %", "average": "= 105" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab233614-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233614-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233614-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB233614-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233614-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB233614-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB233614-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TRAIL R2 Capture Antibody", "number":"AB233614-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TRAIL R2 Lyophilized Recombinant Protein", "number":"AB233614-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB233614-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TRAIL R2 Detector Antibody", "number":"AB233614-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB233614-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DR5 also known as Death Receptor 5 TRAIL-R2 or TNFRSF10B is a protein critical in apoptosis. It is a transmembrane receptor with a mass of approximately 54 kDa. Researchers find it expressed mostly in tissues such as lung colon and prostate. DR5 functions by binding to its ligand TRAIL triggering the extrinsic apoptotic pathway. Through this mechanism DR5 initiates a cascade of caspase activation that leads to cell death.
Biological function summary

DR5 plays a significant role in the regulation of apoptosis particularly in cancer cells. It is a component of the TRAIL receptor complex which includes several other death receptors like DR4. This complex formation allows DR5 to mediate apoptotic signals more efficiently. By controlling apoptosis DR5 helps maintain tissue homeostasis and prevents abnormal cell proliferation.

Pathways

DR5 is an important part of the TRAIL signaling pathway and the extrinsic apoptosis pathway. It connects with proteins like FADD and Caspase-8 essential in the apoptotic signaling cascade. By interacting with these proteins DR5 drives the progression of the apoptotic signal ensuring the removal of dysfunctional cells. Such pathways are vital for restraining tumor development and progression.

DR5 exhibits significant relevance to cancer and autoimmune diseases. Its ability to induce apoptosis through the TRAIL pathway keeps oncogenic transformations in check. In cancer DR5 often interfaces with other proteins like Bcl-2 which are involved in cell survival. Furthermore defects or aberrant expressions in DR5 have associations with autoimmune disorders where excessive cell death or survival contributes to disease pathology. DR5's role in these conditions makes it a promising target in therapeutic strategies.
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Product protocols

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Target data

Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed : 10549288). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
See full target information TNFRSF10B
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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