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AB108902

Human Transferrin ELISA Kit

5

(1 Review)

|

(4 Publications)

Human Transferrin ELISA Kit is a sandwich ELISA designed to quantify Human Transferrin with a sensitivity of 0.93 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range - quantifies 1.563 - 100 ng/mL

View Alternative Names

PRO1400, TF, Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin

Key facts

Detection method

Colorimetric

Sample types

Saliva, Milk, Urine, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

>= 0.93 ng/mL

Range

1.563 - 100 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

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Product details

Human Transferrin ELISA Kit ab108902 is a sandwich ELISA to measure Human Transferrin in cerebral spinal fluid (CSF), saliva, urine, milk with a sensitivity of 0.93 ng/ml.

How the assay works

A Tranferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tranferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tranferrin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Transferrin ELISA Kit ab108902 protocol summary

1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.

Get results in 90 minutes with our SimpleStep ELISA® kit

Human Transferrin ELISA Kit (ab187391)
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transferrin also known as serotransferrin or siderophilin is a glycoprotein with a mass of approximately 80 kDa. It is primarily synthesized in the liver and subsequently secreted into the bloodstream. Transferrin has an important role in iron transport and maintains iron homeostasis by binding and delivering iron to various tissues throughout the body. It can bind two ferric ions (Fe3+) in association with an anion usually bicarbonate. In biological fluids transferrin exists in serum plasma and other extracellular fluids.
Biological function summary

Transferrin facilitates the transportation of iron ions. It delivers iron to cells by binding to transferrin receptors on cell surfaces forming a complex that gets internalized via receptor-mediated endocytosis. Inside the endosomes acidic conditions cause iron to release from transferrin enabling its utilization in cellular processes like DNA synthesis and electron transport. Transferrin itself acts independently and does not form part of a larger protein complex. Variants of transferrin include mouse transferrin bovine transferrin and biotinylated transferrin each with similar function across different species.

Pathways

Transferrin operates centrally in iron metabolism and homeostasis pathways. It functions in coordination with the transferrin receptor 1 (TfR1) which facilitates cellular uptake of the transferrin-iron complex. Additionally transferrin plays a role in the hepcidin regulatory pathway. Hepcidin modulates iron homeostasis by decreasing iron absorption in the intestine and controlling iron release from macrophages and hepatocytes. Transferrin's ability to bind iron connects it to other iron-containing proteins such as ferritin which stores excess iron in cells.

Several iron-related conditions can impact transferrin function including anemia and hemochromatosis. Anemia often occurs when there is insufficient iron delivery leading to inadequate hemoglobin synthesis and reduced oxygen transport. Aberrant transferrin receptor activity affects iron uptake in such conditions. Hemochromatosis characterized by iron overload can occur due to mutations in genes like HFE leading to changes in hepcidin regulation and increased intestinal iron absorption. Transferrin levels and saturation are clinical indicators used to assess iron status in such diseases.
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Product protocols

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Target data

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.. (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use.. (Microbial infection) Serves as an iron source for parasite T.brucei (strain 427), which capture TF via its own transferrin receptor ESAG6 : ESAG7 and extract its iron for its own use.
See full target information TF
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 58:4842-4855 PubMed34195939

2021

Higher CSF Ferritin Heavy-Chain (Fth1) and Transferrin Predict Better Neurocognitive Performance in People with HIV.

Applications

Unspecified application

Species

Unspecified reactive species

Harpreet Kaur,William S Bush,Scott L Letendre,Ronald J Ellis,Robert K Heaton,Stephanie M Patton,James R Connor,David C Samuels,Donald R Franklin,Todd Hulgan,Asha R Kallianpur

Scientific reports 10:10935 PubMed32616918

2020

Altered iron metabolism in cystic fibrosis macrophages: the impact of CFTR modulators and implications for Pseudomonas aeruginosa survival.

Applications

Unspecified application

Species

Unspecified reactive species

H F Hazlett,T H Hampton,D S Aridgides,D A Armstrong,J A Dessaint,D L Mellinger,A B Nymon,A Ashare

Fluids and barriers of the CNS 14:11 PubMed28427421

2017

Cerebrospinal fluid (CSF) biomarkers of iron status are associated with CSF viral load, antiretroviral therapy, and demographic factors in HIV-infected adults.

Applications

ELISA

Species

Human

Stephanie M Patton,Quan Wang,Todd Hulgan,James R Connor,Peilin Jia,Zhongming Zhao,Scott L Letendre,Ronald J Ellis,William S Bush,David C Samuels,Donald R Franklin,Harpreet Kaur,Jennifer Iudicello,Igor Grant,Asha R Kallianpur

Journal of neuroinflammation 14:72 PubMed28359324

2017

Cerebrospinal fluid cell-free mitochondrial DNA is associated with HIV replication, iron transport, and mild HIV-associated neurocognitive impairment.

Applications

ELISA

Species

Human

Sanjay R Mehta,Josué Pérez-Santiago,Todd Hulgan,Tyler R C Day,Jill Barnholtz-Sloan,Haley Gittleman,Scott Letendre,Ronald Ellis,Robert Heaton,Stephanie Patton,Jesse D Suben,Donald Franklin,Debralee Rosario,David B Clifford,Ann C Collier,Christina M Marra,Benjamin B Gelman,Justin McArthur,Allen McCutchan,Susan Morgello,David Simpson,James Connor,Igor Grant,Asha Kallianpur
View all publications
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websiteProtocolBooklet
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