AB187391

Human Transferrin ELISA Kit

SimpleStep
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(6 Publications)

Human Transferrin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Transferrin with a sensitivity of 0.39 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

PRO1400, TF, Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin

9 Images

ELISA - Human Transferrin ELISA Kit (AB187391)

ELISA

Supplier Data

ELISA - Human Transferrin ELISA Kit (AB187391)

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Example of human Transferrin standard curve in Sample Diluent NS.

The Transferrin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Serum from seven individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin concentration was determined to be 2.38 mg/mL with a range of 1.54 – 3.2 mg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Example of human Transferrin standard curve in Sample Diluent NS. The Transferrin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Interpolated concentrations of native Transferrin in human serum and plasmas samples.

The concentrations of Transferrin were measured in duplicates, interpolated from the Transferrin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 50,000, plasma (EDTA) 1 : 50,000, plasma (citrate) 1 : 50,000 and plasma (heparin) 1 : 50,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin concentration was determined to be 1.82 mg/mL in serum, 1.84 ng/mL in plasma (EDTA) and 1.86 mg/mL in plasma (citrate) and 1.43 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Human Transferrin standard curve comparison.

Standard Curve comparison between the original Human Transferrin SimpleStep ELISA kit and current Human Transferrin SimpleStep ELISA kit.

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Transferrin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Interpolated concentration of native Transferrin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 50,000 human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Example of human Transferrin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.39 ng/mL

Range

0.63 - 40 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Transferrin ELISA Kit ab187391 is a rapid single-wash 90-min Sandwich ELISA to measure Human Transferrin in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 0.39 ng/mL.

How the assay works

Human Transferrin SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Transferrin ELISA Kit ab187391 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human Transferrin ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Transferrin antibody [EPR20149-108] - BSA and Azide free (Capture) ab244848
- Anti-Transferrin antibody [EPR20149-29] - BSA and Azide free (Detector) ab244848

Transferrins are iron binding transport proteins which can bind two Fe³⁺ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]

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Recovery

[ { "sample": "Serum", "range": "86 - 104 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "95 - 115 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "112 - 116 %", "average": "= 114" }, { "sample": "Citrate plasma", "range": "104 - 121 %", "average": "= 115" }, { "sample": "Cell culture media", "range": "97 - 103 %", "average": "= 101" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transferrin also known as serotransferrin or siderophilin is a glycoprotein with a mass of approximately 80 kDa. It is primarily synthesized in the liver and subsequently secreted into the bloodstream. Transferrin has an important role in iron transport and maintains iron homeostasis by binding and delivering iron to various tissues throughout the body. It can bind two ferric ions (Fe3+) in association with an anion usually bicarbonate. In biological fluids transferrin exists in serum plasma and other extracellular fluids.

Biological function summary

Transferrin facilitates the transportation of iron ions. It delivers iron to cells by binding to transferrin receptors on cell surfaces forming a complex that gets internalized via receptor-mediated endocytosis. Inside the endosomes acidic conditions cause iron to release from transferrin enabling its utilization in cellular processes like DNA synthesis and electron transport. Transferrin itself acts independently and does not form part of a larger protein complex. Variants of transferrin include mouse transferrin bovine transferrin and biotinylated transferrin each with similar function across different species.

Pathways

Transferrin operates centrally in iron metabolism and homeostasis pathways. It functions in coordination with the transferrin receptor 1 (TfR1) which facilitates cellular uptake of the transferrin-iron complex. Additionally transferrin plays a role in the hepcidin regulatory pathway. Hepcidin modulates iron homeostasis by decreasing iron absorption in the intestine and controlling iron release from macrophages and hepatocytes. Transferrin's ability to bind iron connects it to other iron-containing proteins such as ferritin which stores excess iron in cells.

Several iron-related conditions can impact transferrin function including anemia and hemochromatosis. Anemia often occurs when there is insufficient iron delivery leading to inadequate hemoglobin synthesis and reduced oxygen transport. Aberrant transferrin receptor activity affects iron uptake in such conditions. Hemochromatosis characterized by iron overload can occur due to mutations in genes like HFE leading to changes in hepcidin regulation and increased intestinal iron absorption. Transferrin levels and saturation are clinical indicators used to assess iron status in such diseases.

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Product protocols

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Target data

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.. (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use.. (Microbial infection) Serves as an iron source for parasite T.brucei (strain 427), which capture TF via its own transferrin receptor ESAG6 : ESAG7 and extract its iron for its own use.

See full target information TF

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal for equity in health 23:200 PubMed39369256

2024

Biological and contextual determinants of early development in marginalized Roma communities: A research protocol of the RomaREACH study.

Applications

Unspecified application

Species

Unspecified reactive species

Shoshana Chovan,Daniela Fiľakovská Bobáková,Andrea Madarasová Gecková,Beáta Hubková,Gabriela Štrkolcová,Sijmen A Reijneveld,Marlou L A de Kroon

Pharmacogenomics and personalized medicine 14:1055-1068 PubMed34483678

2021

Haematological Indicators of Response to Erythropoietin Therapy in Chronic Renal Failure Patients on Haemodialysis: Impact of Angiotensin-Converting Enzyme rs4343 Gene Polymorphism.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulrahman Hamdan Almaeen,Gomaa Mostafa-Hedeab

Nutrients 12: PubMed32854403

2020

Effects of Dietary Glucose and Fructose on Copper, Iron, and Zinc Metabolism Parameters in Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Nathaniel H O Harder,Bettina Hieronimus,Kimber L Stanhope,Noreene M Shibata,Vivien Lee,Marinelle V Nunez,Nancy L Keim,Andrew Bremer,Peter J Havel,Marie C Heffern,Valentina Medici

Cell stem cell 24:995-1005.e6 PubMed31173718

2019

Human iPSC-Derived Blood-Brain Barrier Chips Enable Disease Modeling and Personalized Medicine Applications.

Applications

Unspecified application

Species

Unspecified reactive species

Gad D Vatine,Riccardo Barrile,Michael J Workman,Samuel Sances,Bianca K Barriga,Matthew Rahnama,Sonalee Barthakur,Magdalena Kasendra,Carolina Lucchesi,Jordan Kerns,Norman Wen,Weston R Spivia,Zhaohui Chen,Jennifer Van Eyk,Clive N Svendsen

American journal of respiratory and critical care medicine 200:209-219 PubMed31051082

2019

The Transferrin Receptor CD71 Delineates Functionally Distinct Airway Macrophage Subsets during Idiopathic Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah J Allden,Patricia P Ogger,Poonam Ghai,Peter McErlean,Richard Hewitt,Richard Toshner,Simone A Walker,Peter Saunders,Shaun Kingston,Philip L Molyneaux,Toby M Maher,Clare M Lloyd,Adam J Byrne

Antimicrobial agents and chemotherapy 60:4471-81 PubMed27161622

2016

Large-Scale Screening and Identification of Novel Ebola Virus and Marburg Virus Entry Inhibitors.

Applications

sELISA

Species

Human

Manu Anantpadma,Jennifer Kouznetsova,Hang Wang,Ruili Huang,Andrey Kolokoltsov,Rajarshi Guha,Aaron R Lindstrom,Olena Shtanko,Anton Simeonov,David J Maloney,Wendy Maury,Douglas J LaCount,Ajit Jadhav,Robert A Davey

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Transferrin ELISA Kit

ELISA - Human Transferrin ELISA Kit (AB187391)

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Sandwich ELISA - Human Transferrin ELISA Kit (AB187391)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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