AB288174

Human Transferrin ELISA Kit

Recombinant
SimpleStep
What is this?

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(2 Publications)

Human Transferrin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Transferrin with a sensitivity of 4.396 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PRO1400, TF, Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin

6 Images

Sandwich ELISA - Human Transferrin ELISA Kit (AB288174)

sELISA

Lab

Sandwich ELISA - Human Transferrin ELISA Kit (AB288174)

Example of human Transferrin standard curve in Sample Diluent NS in 96-well vs. 384-well plate.

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin ELISA Kit (AB288174)

Human Transferrin standard curve comparison. Standard curve comparison between the original Human Transferrin SimpleStep ELISA (ab187391) and current Human Transferrin SimpleStep ELISA (ab288174).

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin ELISA Kit (AB288174)

Interpolated concentrations of Transferrin in normal human serum donors. Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Transferrin concentration was determined to be 2287 ug/mL with a range of 1595- 2940 ug/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin ELISA Kit (AB288174)

Example of human Transferrin standard curve in Sample Diluent NS. Example of human Transferrin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Transferrin ELISA Kit (AB288174)

Interpolated concentration of native Transferrin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 2,000,000 serum. The interpolated dilution factor corrected (to neat) values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin ELISA Kit (AB288174)

Interpolated concentrations of human Transferrin in serum, plasma (EDTA), plasma (citrate), and plasma (heparin). Interpolated concentration of native Transferrin was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 2,000,0000 serum, plasma (EDTA), plasma (citrate), The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.396 pg/mL

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Transferrin ELISA Kit (ab288174) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Transferrin protein in cell culture media, plasma and serum samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Transferrin with 4.396 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" } ]

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Recovery

[ { "sample": "Serum", "range": "104 - 129 %", "average": "= 119" }, { "sample": "EDTA Plasma", "range": "115 - 117 %", "average": "= 116" }, { "sample": "Cell culture media", "range": "89 - 98 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "108 - 113 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "112 - 123 %", "average": "= 118" } ]

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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab288174-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB288174-CMP04" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB288174-CMP12" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288174-CMP10" }, { "size":"2 x 1 Vial", "name":"Human Transferrin Lyophilized Purified Protein", "number":"AB288174-CMP08" }, { "size":"1 x 600 µL", "name":"10X Human Transferrin Capture Antibody", "number":"AB288174-CMP06" }, { "size":"1 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288174-CMP02" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288174-CMP09" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB288174-CMP05" }, { "size":"1 x 600 µL", "name":"10X Human Transferrin Detector Antibody", "number":"AB288174-CMP07" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB288174-CMP11" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288174-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288174-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB288174-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288174-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Transferrin Lyophilized Purified Protein", "number":"AB288174-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Transferrin Capture Antibody", "number":"AB288174-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288174-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288174-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Transferrin Detector Antibody", "number":"AB288174-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288174-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288174-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transferrin also known as serotransferrin or siderophilin is a glycoprotein with a mass of approximately 80 kDa. It is primarily synthesized in the liver and subsequently secreted into the bloodstream. Transferrin has an important role in iron transport and maintains iron homeostasis by binding and delivering iron to various tissues throughout the body. It can bind two ferric ions (Fe3+) in association with an anion usually bicarbonate. In biological fluids transferrin exists in serum plasma and other extracellular fluids.

Biological function summary

Transferrin facilitates the transportation of iron ions. It delivers iron to cells by binding to transferrin receptors on cell surfaces forming a complex that gets internalized via receptor-mediated endocytosis. Inside the endosomes acidic conditions cause iron to release from transferrin enabling its utilization in cellular processes like DNA synthesis and electron transport. Transferrin itself acts independently and does not form part of a larger protein complex. Variants of transferrin include mouse transferrin bovine transferrin and biotinylated transferrin each with similar function across different species.

Pathways

Transferrin operates centrally in iron metabolism and homeostasis pathways. It functions in coordination with the transferrin receptor 1 (TfR1) which facilitates cellular uptake of the transferrin-iron complex. Additionally transferrin plays a role in the hepcidin regulatory pathway. Hepcidin modulates iron homeostasis by decreasing iron absorption in the intestine and controlling iron release from macrophages and hepatocytes. Transferrin's ability to bind iron connects it to other iron-containing proteins such as ferritin which stores excess iron in cells.

Several iron-related conditions can impact transferrin function including anemia and hemochromatosis. Anemia often occurs when there is insufficient iron delivery leading to inadequate hemoglobin synthesis and reduced oxygen transport. Aberrant transferrin receptor activity affects iron uptake in such conditions. Hemochromatosis characterized by iron overload can occur due to mutations in genes like HFE leading to changes in hepcidin regulation and increased intestinal iron absorption. Transferrin levels and saturation are clinical indicators used to assess iron status in such diseases.

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Product protocols

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Target data

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.. (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use.. (Microbial infection) Serves as an iron source for parasite T.brucei (strain 427), which capture TF via its own transferrin receptor ESAG6 : ESAG7 and extract its iron for its own use.

See full target information TF

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Publications (2)

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European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 198:114242 PubMed38442794

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Species

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Increased Levels of Circulating Iron-Albumin Complexes in Peripheral Arterial Disease Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabetta Schiano,Enrico Cappello,Domenico Cecere,Francesco Pompeo,Ettore Novellino,Mariano Stornaiuolo,Marcello Izzo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Transferrin ELISA Kit

Sandwich ELISA - Human Transferrin ELISA Kit (AB288174)

Sandwich ELISA - Human Transferrin ELISA Kit (AB288174)

Sandwich ELISA - Human Transferrin ELISA Kit (AB288174)

Sandwich ELISA - Human Transferrin ELISA Kit (AB288174)

Sandwich ELISA - Human Transferrin ELISA Kit (AB288174)

Sandwich ELISA - Human Transferrin ELISA Kit (AB288174)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise