Human Transferrin Receptor ELISA Kit (CD71) is a single-wash 90-min Simplestep used to quantify Human Transferrin Receptor (CD71) with a sensitivity of 18 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 53.13 - 3400 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 18 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738). Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the hereditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738). When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738). When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738). Mediates uptake of NICOL1 into fibroblasts where it may regulate extracellular matrix production (By similarity). (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Alternative names
CD71, Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, TFRC
What's included?
Recommended products
Human Transferrin Receptor ELISA Kit (CD71) is a single-wash 90-min Simplestep used to quantify Human Transferrin Receptor (CD71) with a sensitivity of 18 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 53.13 - 3400 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 18 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 7 | mean - | SD - | C.V. 3.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 94 | Range 90 - 101 % |
Sample type Serum | Average % = 86 | Range 82 - 94 % |
Sample type EDTA Plasma | Average % = 93 | Range 82 - 100 % |
Sample type Saliva | Average % = 75 | Range 73 - 76 % |
Sample type Urine | Average % = 89 | Range 88 - 90 % |
Sample type Heparin Plasma | Average % = 106 | Range 103 - 108 % |
Sample type Citrate plasma | Average % = 110 | Range 107 - 112 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Transferrin Receptor ELISA kit (ab272469) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Transferrin Receptor protein in serum, plasma, urine, saliva, cell culture supernatant and cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Transferrin Receptor with 18 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Transferrin receptor, also known as CD71 TfR1, is a 760 amino acid, transmembrane glycoprotein of plasma membrane composed of two disulfide-linked monomers encoded by the gene TFRC. Transferrin receptor is necessary to regulate iron transport into cells from transferrin by receptor mediated endocytosis. The soluble form of transferrin receptor is due to the proteolysis of Arg-100 in the extracellular domain. Measurements of soluble transferrin receptor may be an indication of iron deficiency due to chronic diseases
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The transferrin receptor commonly referred to as TfR or CD71 is an integral membrane protein that facilitates the uptake of transferrin-bound iron into cells. This receptor has a molecular weight of around 95 kDa and often exists as a homodimer on the cell surface. It is widely expressed in many tissues especially in erythroid precursors and rapidly dividing cells. Alternate names for this receptor include TfR1 and TfR2 though they have distinct roles and distributions. Other transmembrane proteins like OX26 and MEM have been studied in relation to the transferrin receptor due to their involvement in drug delivery.
- Biological function summary
TfR plays a critical role in iron homeostasis by mediating the internalization of transferrin and release of iron in the endosomes. It operates as part of the transferrin-transferrin receptor complex facilitating iron assimilation necessary for DNA synthesis and cell growth. Iron release involves acidifying endosomes allowing transferrin to bind with specific cellular receptors including alternate forms like beta 2 transferrin. The process subsequently contributes to erythropoiesis and various metabolic processes by regulating essential cellular iron levels.
- Pathways
The transferrin receptor is central to iron metabolism and the receptor-mediated endocytosis pathway. It tightly interacts with transferrin and intracellular pathways process the iron released from transferrin within endosomes. The receptor's role in this pathway involves a dynamic with other proteins such as HFE and hepcidin. These interactions help control systemic iron levels linking closely to the maintenance of erythroid cell health and proliferation.
- Associated diseases and disorders
Disruptions in transferrin receptor function correlate with anemia and neurodegenerative disorders. In anemia related to iron deficiency impaired TfR activity reduces iron uptake culminating in insufficient erythropoiesis. Altered receptor expression or function also connects to neurological diseases like Alzheimer's where iron dysregulation is a concern. Here the transferrin receptor interacts with proteins like Amyloid precursor protein contributing to disease pathology through improper metal homeostasis.
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7 product images
Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (ab272469)
Human Transferrin Receptor competitor curve comparison.
Standard Curve comparison between Human Transferrin Receptor SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (ab272469)
Human Transferrin Receptor standard curve comparison.
Standard Curve comparison between the original Human Transferrin Receptor SimpleStep ELISA kit (ab217780) and current Human Transferrin Receptor SimpleStep ELISA kit (ab272469).
Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (ab272469)
Example of human Transferrin Receptor standard curve in Sample Diluent NS.
The Transferrin Receptor standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (ab272469)
Interpolated concentrations of native Transferrin Receptor in human serum and plasma samples.
The concentrations of Transferrin Receptor were measured in duplicates, interpolated from the Transferrin Receptor standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1,000, plasma (citrate) 1:1,000, plasma (EDTA) 1:500 and plasma (heparin) 1:1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 2,122 ng/mL in neat serum, 2,234 ng/mL in neat plasma (citrate), 1,714 ng/mL in neat plasma (EDTA) and 1,990 ng/mL in neat plasma (heparin).
Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (ab272469)
Interpolated concentrations of native Transferrin Receptor in human urine, saliva and A549 cell culture supernatant samples.
The concentrations of Transferrin Receptor were measured in duplicates, interpolated from the Transferrin Receptor standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 25%, saliva 12.5%, and A549 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 829 pg/mL in neat urine, 3,217 pg/mL in neat saliva and 1,755 pg/mL in neat A549 supernatant.
Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (ab272469)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 3,747 ng/mL with a range of 2,378 – 6,239 ng/mL.
Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (ab272469)
Interpolated concentrations of native Transferrin Receptor in human A549 cell based on a 25 μg/mL extract load.
The concentrations of Transferrin Receptor were measured in duplicate and interpolated from the Transferrin Receptor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 2,157 pg/mL in A549 cell extract.
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