AB272469

Human Transferrin Receptor ELISA Kit (CD71)

Recombinant
SimpleStep
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Human Transferrin Receptor ELISA Kit (CD71) is a single-wash 90-min Simplestep used to quantify Human Transferrin Receptor (CD71) with a sensitivity of 18 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD71, Transferrin receptor protein 1, TR, TfR, TfR1, Trfr, T9, p90, TFRC

8 Images

ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

ELISA

Supplier Data

ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Interpolated concentrations of native Transferrin Receptor in human urine, saliva and A549 cell culture supernatant samples.

The concentrations of Transferrin Receptor were measured in duplicates, interpolated from the Transferrin Receptor standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 25%, saliva 12.5%, and A549 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 829 pg/mL in neat urine, 3,217 pg/mL in neat saliva and 1,755 pg/mL in neat A549 supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Human Transferrin Receptor competitor curve comparison.

Standard Curve comparison between Human Transferrin Receptor SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Human Transferrin Receptor standard curve comparison.

Standard Curve comparison between the original Human Transferrin Receptor SimpleStep ELISA kit (ab217780) and current Human Transferrin Receptor SimpleStep ELISA kit (ab272469).

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Interpolated concentrations of native Transferrin Receptor in human serum and plasma samples.

The concentrations of Transferrin Receptor were measured in duplicates, interpolated from the Transferrin Receptor standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 1,000, plasma (citrate) 1 : 1,000, plasma (EDTA) 1 : 500 and plasma (heparin) 1 : 1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 2,122 ng/mL in neat serum, 2,234 ng/mL in neat plasma (citrate), 1,714 ng/mL in neat plasma (EDTA) and 1,990 ng/mL in neat plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Example of human Transferrin Receptor standard curve in Sample Diluent NS.

The Transferrin Receptor standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Interpolated concentrations of native Transferrin Receptor in human A549 cell based on a 25 μg/mL extract load.

The concentrations of Transferrin Receptor were measured in duplicate and interpolated from the Transferrin Receptor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 2,157 pg/mL in A549 cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Transferrin Receptor concentration was determined to be 3,747 ng/mL with a range of 2,378 – 6,239 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 18 pg/mL

Range

53.13 - 3400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Transferrin Receptor ELISA Kit (CD71) ab272469 is a rapid single-wash 90-min Sandwich ELISA to measure Human Transferrin Receptor (CD71)in cell culture supernatant, cell lysate, citrate plasma, EDTA plasma, heparin plasma, saliva, serum, urine. This SimpleStep sensitivity is 18 pg/mL.

How the assay works

Human Transferrin Receptor (CD71)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Transferrin Receptor ELISA Kit (CD71) ab272469 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Transferrin receptor, also known as CD71 TfR1, is a 760 amino acid, transmembrane glycoprotein of plasma membrane composed of two disulfide-linked monomers encoded by the gene TFRC. Transferrin receptor is necessary to regulate iron transport into cells from transferrin by receptor mediated endocytosis. The soluble form of transferrin receptor is due to the proteolysis of Arg-100 in the extracellular domain. Measurements of soluble transferrin receptor may be an indication of iron deficiency due to chronic diseases

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 7, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "90 - 101 %", "average": "= 94" }, { "sample": "Serum", "range": "82 - 94 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "82 - 100 %", "average": "= 93" }, { "sample": "Saliva", "range": "73 - 76 %", "average": "= 75" }, { "sample": "Urine", "range": "88 - 90 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "103 - 108 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "107 - 112 %", "average": "= 110" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272469-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272469-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272469-CMP11", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272469-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272469-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272469-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Transferrin Receptor (CD71) Capture Antibody", "number":"AB272469-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Transferrin Receptor (CD71) Lyophilized Recombinant Protein", "number":"AB272469-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB272469-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Transferrin Receptor (CD71) Detector Antibody", "number":"AB272469-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272469-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB272469-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The transferrin receptor commonly referred to as TfR or CD71 is an integral membrane protein that facilitates the uptake of transferrin-bound iron into cells. This receptor has a molecular weight of around 95 kDa and often exists as a homodimer on the cell surface. It is widely expressed in many tissues especially in erythroid precursors and rapidly dividing cells. Alternate names for this receptor include TfR1 and TfR2 though they have distinct roles and distributions. Other transmembrane proteins like OX26 and MEM have been studied in relation to the transferrin receptor due to their involvement in drug delivery.

Biological function summary

TfR plays a critical role in iron homeostasis by mediating the internalization of transferrin and release of iron in the endosomes. It operates as part of the transferrin-transferrin receptor complex facilitating iron assimilation necessary for DNA synthesis and cell growth. Iron release involves acidifying endosomes allowing transferrin to bind with specific cellular receptors including alternate forms like beta 2 transferrin. The process subsequently contributes to erythropoiesis and various metabolic processes by regulating essential cellular iron levels.

Pathways

The transferrin receptor is central to iron metabolism and the receptor-mediated endocytosis pathway. It tightly interacts with transferrin and intracellular pathways process the iron released from transferrin within endosomes. The receptor's role in this pathway involves a dynamic with other proteins such as HFE and hepcidin. These interactions help control systemic iron levels linking closely to the maintenance of erythroid cell health and proliferation.

Disruptions in transferrin receptor function correlate with anemia and neurodegenerative disorders. In anemia related to iron deficiency impaired TfR activity reduces iron uptake culminating in insufficient erythropoiesis. Altered receptor expression or function also connects to neurological diseases like Alzheimer's where iron dysregulation is a concern. Here the transferrin receptor interacts with proteins like Amyloid precursor protein contributing to disease pathology through improper metal homeostasis.

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Product protocols

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Target data

The protein expressed by the gene TFRC is involved in the cellular uptake of iron via receptor-mediated endocytosis, where the transferrin receptor, bound to its ligand, is internalized into specialized endosomes. Following endosomal acidification, iron is released, and the apotransferrin-receptor complex is recycled to the cell surface, where a return to neutral pH results in the loss of apotransferrin's affinity for its receptor. The hereditary hemochromatosis protein HFE competes with transferrin for binding at an overlapping C-terminal site. TFRC positively regulates T and B cell proliferation through iron uptake. It functions as a lipid sensor influencing mitochondrial fusion by modulating the JNK pathway activity. Low dietary levels of stearate promote JNK pathway activation, leading to HUWE1-mediated ubiquitination and degradation of the mitofusin MFN2, inhibiting mitochondrial fusion. High dietary stearate levels result in TFRC stearoylation, which inhibits JNK pathway activation and MFN2 degradation. Additionally, TFRC acts as a receptor for new-world arenaviruses, including Guanarito, Junin, and Machupo viruses. This supplementary information is collated from multiple sources and compiled automatically.

See full target information TFRC

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Respiratory research 25:296 PubMed39097701

2024

Identification of TFRC as a biomarker for pulmonary arterial hypertension based on bioinformatics and experimental verification.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Yang,Yi-Hang Liu,Hai-Kuo Zheng

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Transferrin Receptor ELISA Kit (CD71)

ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Sandwich ELISA - Human Transferrin Receptor ELISA Kit (CD71) (AB272469)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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