AB187398

Human Transketolase ELISA Kit (TKT)

SimpleStep
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(1 Publication)

Human Transketolase ELISA Kit (TKT) is a single-wash 90-min Simplestep used to quantify Human Transketolase (TKT) with a sensitivity of 800 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

Transketolase, TK, TKT

5 Images

Sandwich ELISA - Human Transketolase ELISA Kit (TKT) (AB187398)

sELISA

Supplier Data

Sandwich ELISA - Human Transketolase ELISA Kit (TKT) (AB187398)

Titration of Human tissue homogenate extracts within the working range of the assay.

Background-subtracted data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Transketolase ELISA Kit (TKT) (AB187398)

Example of TKT standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Transketolase ELISA Kit (TKT) (AB187398)

Titration of cell extracts within the working range of the assay.

Background-subtracted data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Transketolase ELISA Kit (TKT) (AB187398)

TKT concentrations in HepG2 and HeLa cells.

Three concentrations (within the working range of the assay) of the cell extracts were analyzed in duplicates with this kit. The concentrations of TKT were interpolated from data values using TKT standard curve and graphed in ng of TKT per mg of extract (mean +/- SD, n=3).

sELISA

Supplier Data

Sandwich ELISA - Human Transketolase ELISA Kit (TKT) (AB187398)

Representative image of Human Transketolase ELISA Kit (TKT) (ab187398)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Transketolase (TKT) Capture Antibody

- 10X Human Transketolase (TKT) Detector Antibody

- Human Transketolase (TKT) Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 800 pg/mL

Range

4.68 - 300 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Transketolase ELISA Kit (TKT) (ab187398) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Transketolase (TKT) protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Transketolase (TKT) with 800 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Transketolase is a key enzyme in the non-oxidative branch of the pentose phosphate pathway that transfers a two-carbon glycoaldehyde unit from ketose-donor to aldose-acceptor sugars. The enzyme is also involved in the photosynthetic Calvin cycle in plants and autotrophic bacteria. Thiamine diphosphate and calcium are essential cofactors in Transketolase catalyzed reactions. In mammals, Transketolase connects the pentose phosphate pathway to glycolysis, feeding excess sugar phosphates into the main carbohydrate metabolic pathways. Its presence is necessary for the production of NADPH, especially in tissues actively engaged in biosyntheses, such as fatty acid synthesis by the liver and mammary glands, and for steroid synthesis by the liver and adrenal glands. Transketolase activity is decreased in deficiency of thiamine, which is generally due to malnutrition, i.e. Wernicke-Korsakoff syndrome.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "HeLa Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.2" }, { "reproducibilityType": "Intra", "sample": "HeLa Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]

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Recovery

[ { "sample": "Cell culture media", "range": "111.9 - 117.7 %", "average": "= 114" }, { "sample": "Goat Serum", "range": "73 - 75.8 %", "average": "= 74.6" }, { "sample": "Serum", "range": "107.2 - 114 %", "average": "= 109.5" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab187398-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB187398-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB187398-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB187398-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB187398-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB187398-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB187398-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB187398-CMP09", "productcode":"" }, { "size":"2 x 300 µL", "name":"10X Human TKT Capture Antibody", "number":"AB187398-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB187398-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB187398-CMP08", "productcode":"" }, { "size":"2 x 300 µL", "name":"10X Human TKT Detector Antibody", "number":"AB187398-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TKT Lyophilized Recombinant Protein", "number":"AB187398-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transketolase (TKT) is an important enzyme that plays an important mechanical role in the pentose phosphate pathway by catalyzing the transfer of ketol groups from ketose donors to aldose acceptors. This process forms part of the non-oxidative segment of the pathway converting ribose 5-phosphate into other sugars equipped for nucleotide synthesis and energy metabolism. TKT is also recognized under the name erythrocyte transketolase as it is found in high abundance in erythrocytes. The enzyme has a molecular mass of around 73 kDa and expresses broadly in human tissues especially within liver and fat cells where it supports carbohydrate metabolism.

Biological function summary

Transketolase participates in critical cellular metabolic processes facilitating cellular growth and repair by supporting the synthesis of nucleotides and amino acids. It does not function alone but rather operates as part of a complex with other enzymes such as transaldolase within the pentose phosphate pathway. The direct activity of erythrocyte transketolase can be essential for maintaining redox balance and providing intermediates that impact cell proliferation and antioxidant defense mechanisms.

Pathways

With regard to pathways involved in carbohydrate metabolism TKT plays an important role in the pentose phosphate pathway and glycolysis. In the pentose phosphate pathway TKT collaborates with enzymes like glucose-6-phosphate dehydrogenase to provide ribose-5-phosphate for nucleic acid synthesis and NADPH for reductive biosynthesis and oxidative stress defense. Its relationship with transaldolase in this pathway showcases a tightly regulated enzymatic network that maintains cellular homeostasis.

TKT is associated with metabolic dysfunctions such as diabetes and certain neurodegenerative diseases including Wernicke's encephalopathy. In diabetes changes in transketolase activity can disturb glucose metabolism and contribute to oxidative stress linking it subconsciously with enzymes like phospholipase C which play roles in lipid signaling and cellular responses. Additionally reduced erythrocyte transketolase activity connects with thiamine deficiency in Wernicke's encephalopathy further affecting brain functions and metabolic pathways relying on proper transketolase function.

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Product protocols

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Target data

Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.

See full target information Transketolase

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 10: PubMed35625744

2022

Carbohydrate Metabolism Parameters of Adult Glial Neoplasms According to Immunohistochemical Profile.

Applications

Unspecified application

Species

Unspecified reactive species

Larisa Obukhova,Olga Nikiforova,Claudia Kontorshchikova,Igor Medyanik,Natalya Orlinskaya,Artem Grishin,Michael Kontorshchikov,Natalya Shchelchkova

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Transketolase ELISA Kit (TKT)

Sandwich ELISA - Human Transketolase ELISA Kit (TKT) (AB187398)

Sandwich ELISA - Human Transketolase ELISA Kit (TKT) (AB187398)

Sandwich ELISA - Human Transketolase ELISA Kit (TKT) (AB187398)

Sandwich ELISA - Human Transketolase ELISA Kit (TKT) (AB187398)

Sandwich ELISA - Human Transketolase ELISA Kit (TKT) (AB187398)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise