AB270884

Human TREM-1 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human TREM-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TREM-1 with a sensitivity of 7.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD354, Triggering receptor expressed on myeloid cells 1, TREM-1, Triggering receptor expressed on monocytes 1, TREM1

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Sandwich ELISA - Human TREM-1 ELISA Kit (AB270884)

sELISA

Supplier Data

Sandwich ELISA - Human TREM-1 ELISA Kit (AB270884)

Interpolated concentrations of native TREM-1 in human saliva.

The concentrations of TREM-1 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 820 pg/mL in saliva.

sELISA

Supplier Data

Sandwich ELISA - Human TREM-1 ELISA Kit (AB270884)

Serum from eight individual healthy TREM-1 gender donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM-1 concentration was determined to be 180 pg/mL with a range of 80 – 269 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human TREM-1 ELISA Kit (AB270884)

Human TREM-1 competitor curve comparison.

Standard Curve comparison between Human TREM-1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human TREM-1 ELISA Kit (AB270884)

Human TREM-1 standard curve comparison.

Standard Curve comparison between the original Human TREM-1 SimpleStep ELISA kit (ab185987) and current Human TREM-1 SimpleStep ELISA kit (ab270884).

sELISA

Supplier Data

Sandwich ELISA - Human TREM-1 ELISA Kit (AB270884)

Example of human TREM-1 standard curve in Sample Diluent NS.

The TREM-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 7.1 pg/mL

Range

31 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human TREM-1 ELISA kit (ab270884) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TREM-1 protein in human serum, plasmas, saliva, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human TREM-1 with 7.1 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

The triggering receptor expressed on myeloid cells (TREM), modulate the innate response either by amplifying or dampening Toll-Like Receptor (TLR) induced signals, and act to fine-tuning the inflammatory response. Trem-1 is a member of the TREM family of receptors encoded by a cluster of genes localized to the Human chromosome 6p21.1. The presence of TREM and TREM-like receptors are expressed on a variety of innate cells of the myeloid lineage including neutrophils, monocytes, macrophages, microglia, dendritic cells, among others. Soluble Trem-1 is thought to arise through the cleavage of the Trem-1 receptor by metalloproteinases, where it acts to negatively regulate TREM receptor signaling through neutralization of the respective ligands and minimize tissue damage due to prolonged inflammatory response.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" } ]

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Recovery

[ { "sample": "Serum", "range": "97 - 101 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "88 - 94 %", "average": "= 90" }, { "sample": "Saliva", "range": "106 - 110 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "95 - 96 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "86 - 96 %", "average": "= 92" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab270884-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB270884-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB270884-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB270884-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB270884-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB270884-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TREM-1 Lyophilized Recombinant Protein", "number":"AB270884-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB270884-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB270884-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TREM-1 Capture Antibody", "number":"AB270884-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TREM-1 Detector Antibody", "number":"AB270884-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB270884-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Triggering receptor expressed on myeloid cells 1 (TREM1) is a transmembrane receptor involved in amplifying inflammatory responses. TREM1 is known alternatively as CD354. This protein has a molecular mass of approximately 30 kDa and is expressed on the surface of neutrophils monocytes and macrophages. The receptor participates in innate immune responses and acts as an amplifier for inflammatory signals. It is often found in tissues that are engaged in inflammatory processes including lungs and intestines.

Biological function summary

TREM1 significantly enhances the immune response by activating myeloid cells. It does not form a standalone unit; instead it works with the adapter protein DNAX-activating protein of 12 kDa (DAP12) which pairs with TREM1 to transduce signals that promote cytokine and chemokine release. Through this interaction the receptor plays a role in the defense against bacterial infection boosting the body's immune response during pathogen assault. This function positions TREM1 as an important player in the regulation of acute inflammation.

Pathways

TREM1 engages significantly in the Toll-like receptor (TLR) signaling pathway and NF-kB pathway. In these pathways TREM1 interacts with various proteins that include TLRs and DAP12 intensifying inflammatory responses. The integration of TREM1 within these pathways suggests that it acts as an important modulator of inflammation capable of enhancing TLR-mediated signals for a stronger immune response.

TREM1 connections extend to sepsis and inflammatory bowel disease (IBD). In the context of sepsis TREM1 expression correlates with increased inflammation and poor prognosis where it interacts with proteins involved in inflammatory pathways such as TNF-α. Concerning IBD TREM1's role in promoting inflammation links it to the pathogenesis of this disease where it could influence the severity and progression of IBD-related inflammation. Given these relationships therapeutic strategies targeting TREM1 could hold potential in modulating these conditions.

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Product protocols

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Target data

Isoform 1. Cell surface receptor that plays important roles in innate and adaptive immunity by amplifying inflammatory responses (PubMed : 10799849, PubMed : 21393102). Upon activation by various ligands such as PGLYRP1, HMGB1 or HSP70, multimerizes and forms a complex with transmembrane adapter TYROBP/DAP12 (PubMed : 17568691, PubMed : 25595774, PubMed : 29568119). In turn, initiates a SYK-mediated cascade of tyrosine phosphorylation, activating multiple downstream mediators such as BTK, MAPK1, MAPK3 or phospholipase C-gamma (PubMed : 14656437, PubMed : 21659545). This cascade promotes the neutrophil- and macrophage-mediated release of pro-inflammatory cytokines and/or chemokines, as well as their migration and thereby amplifies inflammatory responses that are triggered by bacterial and fungal infections (PubMed : 17098818, PubMed : 17568691). By also promoting the amplification of inflammatory signals that are initially triggered by Toll-like receptor (TLR) and NOD-like receptor engagement, plays a major role in the pathophysiology of acute and chronic inflammatory diseases of different etiologies including septic shock and atherosclerosis (PubMed : 11323674, PubMed : 21393102).. Isoform 2. Acts as a decoy receptor, counterbalancing TREM1 pro-inflammatory activity through the neutralization of its ligand.

See full target information TREM1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Annali italiani di chirurgia 95:299-307 PubMed38918963

2024

The Factors for the Occurrence of Pulmonary Infection after Gastrointestinal Surgery and the Construction of a Predictive Model Using sTREM-1 and TIM-4: A Retrospective Study.

Applications

Unspecified application

Species

Unspecified reactive species

Dai Tang,Hua Ge,Zhengquan Tan,Maozhao Yan,Jiacheng Song

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human TREM-1 ELISA Kit

Sandwich ELISA - Human TREM-1 ELISA Kit (AB270884)

Sandwich ELISA - Human TREM-1 ELISA Kit (AB270884)

Sandwich ELISA - Human TREM-1 ELISA Kit (AB270884)

Sandwich ELISA - Human TREM-1 ELISA Kit (AB270884)

Sandwich ELISA - Human TREM-1 ELISA Kit (AB270884)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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