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AB224881

Human TREM2 ELISA Kit

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(22 Publications)

Human TREM2 ELISA Kit is a single-wash 90-min SimpleStep Sandwich ELISA used to quantify Human TREM2 with a sensitivity of 10.5 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout, works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Need to design your own assay: we also offer the conjugation-ready antibody pair
- Cited in over 10 publications

View Alternative Names

Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2, TREM2

16 Images
Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Interpolated concentrations of native TREM2 in Human cerebrospinal fluid and THP-1 cell culture supernatant samples.

The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows : cerebrospinal fluid 25% and THP-1 cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 19.8 ng/mL in cerebrospinal fluid, and 8.4 ng/mL in THP-1 cell culture supernatant.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Example of Human TREM2 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Interpolated concentrations of native TREM2 in Human serum and plasma samples.

The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 23.8 ng/mL in serum, 19.0 ng/mL in plasma (EDTA), 25.3ng/mL in plasma (heparin) and 16.5 ng/mL in plasma (citrate).

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Interpolated concentrations of native TREM2 in human THP-1 cell extract and human placenta tissue extract.

The concentrations of TREM2 were measured in duplicate and interpolated from the TREM2 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 15.7 pg/μg in THP-1 cell extract and 0.34 pg/μg in placenta tissue extract.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR. The TREM2 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Serum from ten individual healthy Human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 30 ng/mL with a range of 13 – 66 ng/mL.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Linearity of dilution.

Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Linearity of dilution.

Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Example of Human TREM2 standard curve in Sample Diluent NS. The TREM2 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Example of human TREM2 standard curve in Sample Diluent NS in 96-well vs. 384-well plate.

Example of human TREM2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Interpolated concentrations of human TREM2 in THP-1 cell extract in 96-well vs. 384-well plates.

Interpolated concentration of native TREM2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 250 µg/mL THP-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Interpolated concentrations of human TREM2 in human serum in 96-well vs. 384-well plates.

Interpolated concentration of native TREM2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 12.5% human serum. The interpolated dilution factor corrected values (to near sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Example of human TREM2 standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.

Example of human TREM2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture extracts, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10.5 pg/mL

Range

78.1 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human TREM2 ELISA Kit ab224881 is a rapid single-wash 90-min ELISA kit to measure Human TREM2 in cerebral spinal fluid (CSF), cell culture extracts, tissue extracts, cell culture media, serum and plasma. This SimpleStep ELISA® sensitivity is 10.5 pg/mL.

How the assay works
Human TREM2 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit recognizes both native and recombinant Human TREM2 protein in serum, plasma, cerebrospinal fluid and cell culture supernatant, cell and tissue extract samples only.

Human TREM2 ELISA Kit ab224881 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human TREM2 ELISA kit ab224881
Human TREM2 ELISA kit ab224881has been used to study the blood brain barrier disruption in anti-NMDA receptor encephalitis(1) and to study TREM2 expression in plasma and CSF of Alzheimer disease patients(2).

References:
(1)Haoxiao Chang et al. 2023, PMID: 37481571,
(2)F. La Rosa et al. 2023, PMID: 37239970

Save your precious samples
Our 384-well Human TREM2 ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human TREM2 Antibody Pair - BSA and Azide free ab244027
- Anti-TREM2 antibody [EPR20243-78] - BSA and Azide free (Capture) ab244673
- Anti-TREM2 antibody [EPR20243-269] - BSA and Azide free (Detector) ab244938

Related and recommended products
Human TREM2 ELISA kit ab224881 is commonly used to measure TREM2 as a marker of neurodegenerative disorders.

Other ELISA kits to study neurodegeneration include:
- Human IL-6 ELISA kit ab17801
- Human CSF-1-R ELISA kit ab230940

TREM2 is immune receptor expressed on the surface of microglia. TREM2 is proteolytically process by ADAM proteases and the soluble TREM2 is released into the extra cellular space. Elevated levels of soluble TREM2 in cerebrospinal fluid is a biomarker associated with increased risk of Alzheimer's disease. ab224881 TREM2 SimpleStep ELISA® is designed against the extracellular portion of TREM2.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" } ]

Recovery

[ { "sample": "Serum", "range": "85 - 104 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "93 - 105 %", "average": "= 101" }, { "sample": "Cerebral Spinal Fluid", "range": "109 - 113 %", "average": "= 111" }, { "sample": "Cell culture extracts", "range": "100 - 117 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "86 - 94 %", "average": "= 91" }, { "sample": "Tissue Extracts", "range": "95 - 101 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "97 - 111 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "90 - 103 %", "average": "= 95" } ]

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Product protocols

Target data

Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed : 10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia (PubMed : 27477018, PubMed : 29518356). Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1 (By similarity). Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia (PubMed : 27477018). Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin (PubMed : 29794134). Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade (By similarity). Required for microglial phagocytosis of apoptotic neurons (PubMed : 24990881). Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain (By similarity). Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide (By similarity). It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB (By similarity). During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling (By similarity). Plays a role in microglial MTOR activation and metabolism (By similarity). Regulates age-related changes in microglial numbers (PubMed : 29752066). Triggers activation of the immune responses in macrophages and dendritic cells (PubMed : 10799849). Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (By similarity). In dendritic cells, receptor of SEMA6D with PLEXNA1 as coreceptor and mediates up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed : 11602640). Involved in the positive regulation of osteoclast differentiation (PubMed : 12925681).
See full target information TREM2

Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Molecular psychiatry 30:4776-4791 PubMed40527900

2025

Cell autonomous microglia defects in a stem cell model of frontotemporal dementia tau.

Applications

Unspecified application

Species

Unspecified reactive species

Abhirami K Iyer,Lisa Vermunt,Farzaneh S Mirfakhar,Miguel Minaya,Mariana Acquarone,Rama Krishna Koppisetti,Arun Renganathan,Shih-Feng You,Emma P Danhash,Kylie J Schache,Anthony Verbeck,Grant Galasso,Scott M Lee,Guangming Huang,Katherine J Miller,Jacob Marsh,Alissa L Nana,Salvatore Spina,William W Seeley,Lea T Grinberg,Sally Temple,Charlotte E Teunissen,Chihiro Sato,Celeste M Karch

European journal of neurology 32:e70107 PubMed40052391

2025

Microglial Activation Visualized by [F]-DPA714 PET Is a Potential Marker of Severity and Prognosis for Anti-LGI1 Encephalitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingguo Wang,Jingjie Ge,Bo Deng,Huamei Lin,Wenbo Yang,Tianyang Sheng,Weijun Tang,Hai Yu,Xiang Zhang,Yarong Li,Xiaoni Liu,Chuantao Zuo,Xiangjun Chen

Journal of neuroinflammation 22:26 PubMed39891235

2025

Rescue of in vitro models of CSF1R-related adult-onset leukodystrophy by iluzanebart: mechanisms and therapeutic implications of TREM2 agonism.

Applications

Unspecified application

Species

Unspecified reactive species

Kelley C Larson,Frederick W Gergits,Abigail J Renoux,Elizabeth J Weisman,Borislav Dejanovic,Liyue Huang,Bhaumik Pandya,Donald G McLaren,Berkley A Lynch,Richard Fisher,Evan Thackaberry,David Gray,Francois Gaudreault,Christian Mirescu

Frontiers in neurology 15:1515252 PubMed39722698

2024

sTREM2 cerebrospinal fluid levels are a potential biomarker in amyotrophic lateral sclerosis and associate with UMN burden.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Jiao,Jing Yang,Wenjing Wang,Xiangyi Liu,Yu Fu,Dongsheng Fan

International journal of surgery (London, England) 110:6962-6971 PubMed39166950

2024

Blood-brain barrier-associated biomarker correlated with cerebral small vessel disease and shunt outcome in normal pressure hydrocephalus: a prospective cohort study.

Applications

Unspecified application

Species

Unspecified reactive species

Pao-Hui Tseng,Li-Chuan Huang,Xiang-Ling Huang,Bor-Ren Huang,Shinn-Zong Lin,Sheng-Tzung Tsai,Hsin-Yi Huang

Frontiers in aging neuroscience 16:1339163 PubMed39081396

2024

Association of modified dementia risk score with cerebrospinal fluid biomarkers and cognition in adults without dementia.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong-Yao Li,Yan Fu,Xin-Jing Cui,Zuo-Teng Wang,Lan Tan

Journal of neuroinflammation 20:172 PubMed37481571

2023

Elevated blood and cerebrospinal fluid biomarkers of microglial activation and blood‒brain barrier disruption in anti-NMDA receptor encephalitis.

Applications

Unspecified application

Species

Unspecified reactive species

Haoxiao Chang,Jia Ma,Kai Feng,Ning Feng,Xinxin Wang,Jiali Sun,Tianshu Guo,Yuzhen Wei,Yun Xu,Huabing Wang,Linlin Yin,Xinghu Zhang

International journal of molecular sciences 24: PubMed37239970

2023

TREM2 Expression and Amyloid-Beta Phagocytosis in Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca La Rosa,Simone Agostini,Federica Piancone,Ivana Marventano,Ambra Hernis,Chiara Fenoglio,Daniela Galimberti,Elio Scarpini,Marina Saresella,Mario Clerici

Gastro hep advances 2:711-720 PubMed39129874

2023

Obese Patients With Nonalcoholic Fatty Liver Disease Have an Increase in Soluble Plasma CD163 and a Concurrent Decrease in Hepatic Expression of CD163.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Kløjgaard Skytthe,Felix Boel Pedersen,Charlotte Wilhelmina Wernberg,Vineesh Indira Chandran,Aleksander Krag,Tina Di Caterino,Samuel Coelho Mandacaru,Blagoy Blagoev,Mette Munk Lauridsen,Sönke Detlefsen,Jonas Heilskov Graversen,Søren Kragh Moestrup

CNS neuroscience & therapeutics 29:1657-1666 PubMed36815315

2023

Association of rs2062323 in the TREM1 gene with Alzheimer's disease and cerebrospinal fluid-soluble TREM2.

Applications

Unspecified application

Species

Unspecified reactive species

Zuo-Teng Wang,Yan Fu,Shi-Dong Chen,Yu-Yuan Huang,Ya-Hui Ma,Yan-Jiang Wang,Lan Tan,Jin-Tai Yu
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