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AB224881

Human TREM2 ELISA Kit

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(15 Publications)

Human TREM2 ELISA Kit is a single-wash 90-min SimpleStep Sandwich ELISA used to quantify Human TREM2 with a sensitivity of 10.5 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout, works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Need to design your own assay: we also offer the conjugation-ready antibody pair
- Cited in over 10 publications

View Alternative Names

Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2, TREM2

16 Images
Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Interpolated concentrations of native TREM2 in Human cerebrospinal fluid and THP-1 cell culture supernatant samples.

The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows : cerebrospinal fluid 25% and THP-1 cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 19.8 ng/mL in cerebrospinal fluid, and 8.4 ng/mL in THP-1 cell culture supernatant.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Example of Human TREM2 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Interpolated concentrations of native TREM2 in Human serum and plasma samples.

The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 23.8 ng/mL in serum, 19.0 ng/mL in plasma (EDTA), 25.3ng/mL in plasma (heparin) and 16.5 ng/mL in plasma (citrate).

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Interpolated concentrations of native TREM2 in human THP-1 cell extract and human placenta tissue extract.

The concentrations of TREM2 were measured in duplicate and interpolated from the TREM2 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 15.7 pg/μg in THP-1 cell extract and 0.34 pg/μg in placenta tissue extract.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR. The TREM2 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Serum from ten individual healthy Human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 30 ng/mL with a range of 13 – 66 ng/mL.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Linearity of dilution.

Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Linearity of dilution.

Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Supplier Data

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Example of Human TREM2 standard curve in Sample Diluent NS. The TREM2 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Example of human TREM2 standard curve in Sample Diluent NS in 96-well vs. 384-well plate.

Example of human TREM2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Interpolated concentrations of human TREM2 in THP-1 cell extract in 96-well vs. 384-well plates.

Interpolated concentration of native TREM2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 250 µg/mL THP-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Interpolated concentrations of human TREM2 in human serum in 96-well vs. 384-well plates.

Interpolated concentration of native TREM2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 12.5% human serum. The interpolated dilution factor corrected values (to near sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

Example of human TREM2 standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.

Example of human TREM2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture extracts, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 10.5 pg/mL

Range

78.1 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Human TREM2 ELISA Kit ab224881 is a rapid single-wash 90-min ELISA kit to measure Human TREM2 in cerebral spinal fluid (CSF), cell culture extracts, tissue extracts, cell culture media, serum and plasma. This SimpleStep ELISA® sensitivity is 10.5 pg/mL.

How the assay works
Human TREM2 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit recognizes both native and recombinant Human TREM2 protein in serum, plasma, cerebrospinal fluid and cell culture supernatant, cell and tissue extract samples only.

Human TREM2 ELISA Kit ab224881 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human TREM2 ELISA kit ab224881
Human TREM2 ELISA kit ab224881has been used to study the blood brain barrier disruption in anti-NMDA receptor encephalitis(1) and to study TREM2 expression in plasma and CSF of Alzheimer disease patients(2).

References:
(1)Haoxiao Chang et al. 2023, PMID: 37481571,
(2)F. La Rosa et al. 2023, PMID: 37239970

Save your precious samples
Our 384-well Human TREM2 ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human TREM2 Antibody Pair - BSA and Azide free ab244027
- Anti-TREM2 antibody [EPR20243-78] - BSA and Azide free (Capture) ab244673
- Anti-TREM2 antibody [EPR20243-269] - BSA and Azide free (Detector) ab244938

Related and recommended products
Human TREM2 ELISA kit ab224881 is commonly used to measure TREM2 as a marker of neurodegenerative disorders.

Other ELISA kits to study neurodegeneration include:
- Human IL-6 ELISA kit ab17801
- Human CSF-1-R ELISA kit ab230940

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" } ]
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Recovery

[ { "sample": "Serum", "range": "85 - 104 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "93 - 105 %", "average": "= 101" }, { "sample": "Cerebral Spinal Fluid", "range": "109 - 113 %", "average": "= 111" }, { "sample": "Cell culture extracts", "range": "100 - 117 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "86 - 94 %", "average": "= 91" }, { "sample": "Tissue Extracts", "range": "95 - 101 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "97 - 111 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "90 - 103 %", "average": "= 95" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

The protein expressed by gene TREM2 forms a receptor signaling complex with TYROBP, facilitating signaling and cell activation upon ligand binding. It serves as a receptor for amyloid-beta protein 42, mediating its uptake and degradation by microglia, and also acts as a receptor for lipoprotein particles such as LDL, VLDL, HDL, and various apolipoproteins, enhancing their uptake in microglia. TREM2 binds phospholipids, preferably anionic lipids, and is necessary for microglial phagocytosis of apoptotic neurons. It regulates changes in microglial numbers with age and activates immune responses in macrophages and dendritic cells, where it plays a role in chemokine receptor CCR7 up-regulation, as well as dendritic cell maturation and survival. Additionally, TREM2 is involved in positively regulating osteoclast differentiation. This supplementary information is collated from multiple sources and compiled automatically.
See full target information TREM2
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 20:172 PubMed37481571

2023

Elevated blood and cerebrospinal fluid biomarkers of microglial activation and blood‒brain barrier disruption in anti-NMDA receptor encephalitis.

Applications

Unspecified application

Species

Unspecified reactive species

Haoxiao Chang,Jia Ma,Kai Feng,Ning Feng,Xinxin Wang,Jiali Sun,Tianshu Guo,Yuzhen Wei,Yun Xu,Huabing Wang,Linlin Yin,Xinghu Zhang

International journal of molecular sciences 24: PubMed37239970

2023

TREM2 Expression and Amyloid-Beta Phagocytosis in Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca La Rosa,Simone Agostini,Federica Piancone,Ivana Marventano,Ambra Hernis,Chiara Fenoglio,Daniela Galimberti,Elio Scarpini,Marina Saresella,Mario Clerici

CNS neuroscience & therapeutics 29:1657-1666 PubMed36815315

2023

Association of rs2062323 in the TREM1 gene with Alzheimer's disease and cerebrospinal fluid-soluble TREM2.

Applications

Unspecified application

Species

Unspecified reactive species

Zuo-Teng Wang,Yan Fu,Shi-Dong Chen,Yu-Yuan Huang,Ya-Hui Ma,Yan-Jiang Wang,Lan Tan,Jin-Tai Yu

BMC anesthesiology 22:413 PubMed36585610

2022

Anesthesia and surgery-induced elevation of CSF sTREM2 is associated with early cognitive dysfunction after thoracoabdominal aortic dissection surgery.

Applications

Unspecified application

Species

Unspecified reactive species

Kexin Wang,Xuezhao Cao,Zhe Li,Sidan Liu,Yongjian Zhou,Lili Guo,Pengli Li

Annals of clinical and translational neurology 9:1752-1763 PubMed36317226

2022

Association between air pollution and CSF sTREM2 in cognitively normal older adults: The CABLE study.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Li,Ya-Hui Ma,Yan Fu,Jia-Yao Liu,He-Ying Hu,Yong-Li Zhao,Liang-Yu Huang,Lan Tan

Frontiers in pediatrics 10:908527 PubMed36313865

2022

High levels of cerebrospinal fluid soluble triggering receptor expressed on myeloid cells 2 might be a biomarker of activity in pediatric patients with MOG-AD.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Zhou,Weihua Zhang,Changhong Ren,Ji Zhou,Haoxiao Chang,Xiaotun Ren

Cancers 14: PubMed36230558

2022

TREM2 Is Associated with Advanced Stages and Inferior Prognosis in Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ann-Kristin Struckmeier,Anne Radermacher,Michael Fehrenz,Dalia Alansary,Philipp Wartenberg,Mathias Wagner,Anja Scheller,Jochen Hess,Julius Moratin,Christian Freudlsperger,Jürgen Hoffmann,Lorenz Thurner,Klaus Roemer,Kolja Freier,Dominik Horn

Hepatology (Baltimore, Md.) 77:558-572 PubMed35712786

2022

Circulating TREM2 as a noninvasive diagnostic biomarker for NASH in patients with elevated liver stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Vineesh Indira Chandran,Charlotte Wilhelmina Wernberg,Mette Munk Lauridsen,Maria Kløjgaard Skytthe,Sofie Marchsteiner Bendixen,Frederik Tibert Larsen,Camilla Dalby Hansen,Lea Ladegaard Grønkjær,Majken Storm Siersbæk,Tina Di Caterino,Sönke Detlefsen,Holger Jon Møller,Lars Grøntved,Kim Ravnskjaer,Søren Kragh Moestrup,Maja Sofie Thiele,Aleksander Krag,Jonas Heilskov Graversen

Translational psychiatry 12:140 PubMed35379792

2022

sTREM2 mediates the associations of minimal depressive symptoms with amyloid pathology in prodromal Alzheimer's disease: The CABLE study.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Bo Wang,Yan Sun,Ya-Hui Ma,Yan Fu,Hao Hu,Wei Xu,Zuo-Teng Wang,Ling-Zhi Ma,Lan Tan,Jin-Tai Yu

Neurotoxicity research 39:1372-1380 PubMed34097185

2021

Associations of Sleep Characteristics with Cerebrospinal Fluid sTREM2 in Cognitively Normal Older Adults: the CABLE Study.

Applications

Unspecified application

Species

Unspecified reactive species

He-Ying Hu,Ling-Zhi Ma,Hao Hu,Yan-Lin Bi,Ya-Hui Ma,Xue-Ning Shen,Ya-Nan Ou,Qiang Dong,Lan Tan,Jin-Tai Yu
View all publications
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