Human TREM2 ELISA Kit ab224881 is a single-wash 90-min SimpleStep ELISA used to quantify Human TREM2 with a sensitivity of 10.5 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human TREM2 sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Validated in a range of biological samples including CSF
Colorimetric
Cerebral Spinal Fluid, Cell culture extracts, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
78.1 - 5000 pg/mL
1h 30m
= 10.5 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia (PubMed:27477018, PubMed:29518356). Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1 (By similarity). Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia (PubMed:27477018). Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin (PubMed:29794134). Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade (By similarity). Required for microglial phagocytosis of apoptotic neurons (PubMed:24990881). Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain (By similarity). Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide (By similarity). It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB (By similarity). During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling (By similarity). Plays a role in microglial MTOR activation and metabolism (By similarity). Regulates age-related changes in microglial numbers (PubMed:29752066). Triggers activation of the immune responses in macrophages and dendritic cells (PubMed:10799849). Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (By similarity). In dendritic cells, receptor of SEMA6D with PLEXNA1 as coreceptor and mediates up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed:11602640). Involved in the positive regulation of osteoclast differentiation (PubMed:12925681).
Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2, TREM2
Human TREM2 ELISA Kit ab224881 is a single-wash 90-min SimpleStep ELISA used to quantify Human TREM2 with a sensitivity of 10.5 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human TREM2 sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Validated in a range of biological samples including CSF
Colorimetric
Cerebral Spinal Fluid, Cell culture extracts, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
78.1 - 5000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 10.5 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 6.3 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 4.9 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 93 | Range 85 - 104 % |
Sample type EDTA Plasma | Average % = 101 | Range 93 - 105 % |
Sample type Cerebral Spinal Fluid | Average % = 111 | Range 109 - 113 % |
Sample type Cell culture extracts | Average % = 109 | Range 100 - 117 % |
Sample type Cell culture media | Average % = 91 | Range 86 - 94 % |
Sample type Tissue Extracts | Average % = 99 | Range 95 - 101 % |
Sample type Heparin Plasma | Average % = 103 | Range 97 - 111 % |
Sample type Citrate plasma | Average % = 95 | Range 90 - 103 % |
Blue Ice
+4°C
+4°C
+4°C
Human TREM2 ELISA Kit (ab224881) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TREM2 protein in cell culture extracts, cell culture media, cerebral spinal fluid, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TREM2 with 10.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY This kit recognizes both native and recombinant Human TREM2 protein in serum, plasma, cerebrospinal fluid and cell culture supernatant, cell and tissue extract samples only.
SPECIES REACTIVITY This kit recognizes Human TREM2 protein.
Other species reactivity was determined by measuring 25% serum samples of various species, interpolating the protein concentrations from the Human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in Human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse, Rat and cow.
TREM2 is immune receptor expressed on the surface of microglia. TREM2 is proteolytically process by ADAM proteases and the soluble TREM2 is released into the extra cellular space. Elevated levels of soluble TREM2 in cerebrospinal fluid is a biomarker associated with increased risk of Alzheimer's disease. ab224881 TREM2 SimpleStep ELISA® is designed against the extracellular portion of TREM2.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
This supplementary information is collated from multiple sources and compiled automatically.
TREM2 also known as Triggering Receptor Expressed on Myeloid Cells 2 functions as a receptor with an important role in the immune system. This protein weighing about 230-290 kDa mostly expresses in myeloid cells which include macrophages monocytes and microglia. It serves as an important player in signaling for the activation of these cells. Researchers often study TREM2's functions through various species including cynomolgus monkeys to understand its implications better.
TREM2 significantly influences immune responses by participating in cellular clearance functions such as phagocytosis. It forms a receptor complex with DAP12 which transduces signals leading to the activation of immune responses. TREM2 aids in regulating inflammatory responses and ensures the maintenance of tissue homeostasis in healthy and diseased states. Its role extends to the control of lipid metabolism particularly in the central nervous system.
Scientific studies link TREM2 to the immune-inflammatory pathway and the neurodegenerative pathway. Within these pathways TREM2 interacts notably with proteins such as DAP12 and SYK. Through these interactions TREM2 contributes to signaling cascades that modulate inflammation and neurodegenerative processes within the brain supporting cellular communication and survival.
TREM2's functionality connects significantly with Alzheimer's disease and various inflammatory conditions. In Alzheimer's disease mutations in TREM2 alter its normal activity potentially increasing neuroinflammation and advancing disease progression. Additionally collaborations between TREM2 and ApoE proteins further highlight its involvement in lipid regulation within neurodegenerative conditions. Understanding TREM2’s role can pave the way for targeted therapeutic approaches in these disorders.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Example of Human TREM2 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR. The TREM2 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed
Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Example of Human TREM2 standard curve in Sample Diluent NS. The TREM2 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of native TREM2 in Human serum and plasma samples.
The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 23.8 ng/mL in serum, 19.0 ng/mL in plasma (EDTA), 25.3ng/mL in plasma (heparin) and 16.5 ng/mL in plasma (citrate).
Serum from ten individual healthy Human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 30 ng/mL with a range of 13 – 66 ng/mL.
Interpolated concentrations of native TREM2 in Human cerebrospinal fluid and THP-1 cell culture supernatant samples.
The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cerebrospinal fluid 25% and THP-1 cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 19.8 ng/mL in cerebrospinal fluid, and 8.4 ng/mL in THP-1 cell culture supernatant.
Interpolated concentrations of native TREM2 in human THP-1 cell extract and human placenta tissue extract.
The concentrations of TREM2 were measured in duplicate and interpolated from the TREM2 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 15.7 pg/μg in THP-1 cell extract and 0.34 pg/μg in placenta tissue extract.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Linearity of dilution.
Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Linearity of dilution.
Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Example of human TREM2 standard curve in Sample Diluent NS in 96-well vs. 384-well plate.
Example of human TREM2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of human TREM2 in THP-1 cell extract in 96-well vs. 384-well plates.
Interpolated concentration of native TREM2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 250 µg/mL THP-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Interpolated concentrations of human TREM2 in human serum in 96-well vs. 384-well plates.
Interpolated concentration of native TREM2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 12.5% human serum. The interpolated dilution factor corrected values (to near sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Example of human TREM2 standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.
Example of human TREM2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com