Human TREM2 ELISA Kit, colorimetric, 90-min ELISA (ab224881)

Human TREM2 ELISA Kit is a single-wash 90-min SimpleStep Sandwich ELISA used to quantify Human TREM2 with a sensitivity of 10.5 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout, works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Need to design your own assay: we also offer the conjugation-ready antibody pair
- Cited in over 10 publications

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Sandwich ELISA - Human TREM2 ELISA Kit (AB224881), expandable thumbnail

Publications

Key facts

Detection method: Colorimetric
Sample types: Cerebral Spinal Fluid, Cell culture extracts, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 78.1 - 5000 pg/mL
Assay time: 1h 30m
Sensitivity: = 10.5 pg/mL

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Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Target data

See full target information TREM2

Function

The protein expressed by gene TREM2 forms a receptor signaling complex with TYROBP, facilitating signaling and cell activation upon ligand binding. It serves as a receptor for amyloid-beta protein 42, mediating its uptake and degradation by microglia, and also acts as a receptor for lipoprotein particles such as LDL, VLDL, HDL, and various apolipoproteins, enhancing their uptake in microglia. TREM2 binds phospholipids, preferably anionic lipids, and is necessary for microglial phagocytosis of apoptotic neurons. It regulates changes in microglial numbers with age and activates immune responses in macrophages and dendritic cells, where it plays a role in chemokine receptor CCR7 up-regulation, as well as dendritic cell maturation and survival. Additionally, TREM2 is involved in positively regulating osteoclast differentiation. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2, TREM2

What's included?

1 x 384 Tests

Components

10X Human TREM2 Capture Antibody

1 x 600 µL

10X Human TREM2 Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

384 well CaptSure™ microplates

1 x 1 Unit

50X Cell Extraction Enhancer Solution (ab193971)

1 x 1 mL

5X Cell Extraction Buffer PTR (ab193970)

1 x 50 mL

Antibody Diluent CPI2

1 x 6 mL

Human TREM2 Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent NS (ab193972)

1 x 250 mL

Stop Solution

2 x 12 mL

TMB Development Solution

2 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Cerebral Spinal Fluid, Cell culture extracts, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 78.1 - 5000 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 10.5 pg/mL

Precision

Intra assay

Sample	n	C.V.
Sample Serum	n 5	C.V. 6.3

Inter assay

Sample	n	C.V.
Sample Serum	n 3	C.V. 4.9

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Serum	Average % = 93	Range 85 - 104 %
Sample type EDTA Plasma	Average % = 101	Range 93 - 105 %
Sample type Cerebral Spinal Fluid	Average % = 111	Range 109 - 113 %
Sample type Cell culture extracts	Average % = 109	Range 100 - 117 %
Sample type Cell culture media	Average % = 91	Range 86 - 94 %
Sample type Tissue Extracts	Average % = 99	Range 95 - 101 %
Sample type Heparin Plasma	Average % = 103	Range 97 - 111 %
Sample type Citrate plasma	Average % = 95	Range 90 - 103 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human TREM2 ELISA Kit ab224881 is a rapid single-wash 90-min ELISA kit to measure Human TREM2 in cerebral spinal fluid (CSF), cell culture extracts, tissue extracts, cell culture media, serum and plasma. This SimpleStep ELISA® sensitivity is 10.5 pg/mL.

How the assay works

Human TREM2 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant Human TREM2 protein in serum, plasma, cerebrospinal fluid and cell culture supernatant, cell and tissue extract samples only.

Human TREM2 ELISA Kit ab224881 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human TREM2 ELISA kit ab224881

Human TREM2 ELISA kit ab224881has been used to study the blood brain barrier disruption in anti-NMDA receptor encephalitis⁽¹⁾ and to study TREM2 expression in plasma and CSF of Alzheimer disease patients⁽²⁾.

References:

⁽¹⁾Haoxiao Chang et al. 2023, PMID: 37481571,
⁽²⁾F. La Rosa et al. 2023, PMID: 37239970

Save your precious samples

Our 384-well Human TREM2 ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human TREM2 Antibody Pair - BSA and Azide free Human TREM2 Antibody Pair - BSA and Azide free ab244027
- Anti-TREM2 antibody [EPR20243-78] - BSA and Azide free (Capture) Anti-TREM2 antibody [EPR20243-78] - BSA and Azide free (Capture) ab244673
- Anti-TREM2 antibody [EPR20243-269] - BSA and Azide free (Detector) Anti-TREM2 antibody [EPR20243-269] - BSA and Azide free (Detector) ab244938

Related and recommended products

Human TREM2 ELISA kit ab224881 is commonly used to measure TREM2 as a marker of neurodegenerative disorders.

Other ELISA kits to study neurodegeneration include:

- Human IL-6 ELISA kit ab17801
- Human CSF-1-R ELISA kit Human CSF-1-R ELISA Kit ab230940

TREM2 is immune receptor expressed on the surface of microglia. TREM2 is proteolytically process by ADAM proteases and the soluble TREM2 is released into the extra cellular space. Elevated levels of soluble TREM2 in cerebrospinal fluid is a biomarker associated with increased risk of Alzheimer's disease. ab224881 TREM2 SimpleStep ELISA® is designed against the extracellular portion of TREM2.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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16 product images

Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Interpolated concentrations of native TREM2 in Human cerebrospinal fluid and THP-1 cell culture supernatant samples.
The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cerebrospinal fluid 25% and THP-1 cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 19.8 ng/mL in cerebrospinal fluid, and 8.4 ng/mL in THP-1 cell culture supernatant.
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Example of Human TREM2 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR. The TREM2 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Example of Human TREM2 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Example of Human TREM2 standard curve in Sample Diluent NS. The TREM2 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Interpolated concentrations of native TREM2 in Human serum and plasma samples.
The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 23.8 ng/mL in serum, 19.0 ng/mL in plasma (EDTA), 25.3ng/mL in plasma (heparin) and 16.5 ng/mL in plasma (citrate).
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Serum from ten individual healthy Human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 30 ng/mL with a range of 13 – 66 ng/mL.
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Interpolated concentrations of native TREM2 in human THP-1 cell extract and human placenta tissue extract.
The concentrations of TREM2 were measured in duplicate and interpolated from the TREM2 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 15.7 pg/μg in THP-1 cell extract and 0.34 pg/μg in placenta tissue extract.
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Linearity of dilution.
Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Linearity of dilution.
Native TREM2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Example of human TREM2 standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.
Example of human TREM2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Example of human TREM2 standard curve in Sample Diluent NS in 96-well vs. 384-well plate.
Example of human TREM2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Interpolated concentrations of human TREM2 in THP-1 cell extract in 96-well vs. 384-well plates.
Interpolated concentration of native TREM2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 250 µg/mL THP-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human TREM2 ELISA Kit (ab224881)
Interpolated concentrations of human TREM2 in human serum in 96-well vs. 384-well plates.
Interpolated concentration of native TREM2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 12.5% human serum. The interpolated dilution factor corrected values (to near sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.