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AB325449

Human TREM2 ELISA Kit, Chemiluminescent

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Human TREM2 ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Human TREM2 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :8.23 - 6000 pg/mL with a sensitivity of 2.24 pg/mL
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2, TREM2

8 Images
Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)

Interpolated concentrations of native TREM2 in human THP-1 cell extract and human placenta tissue extract was measured in duplicate. Undiluted samples are as follows : THP-1 extracts 250 µg/mL and human placenta 1,000 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)

Interpolated concentrations of native TREM2 in Human cerebrospinal fluid and THP-1 cell culture supernatant samples. The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows : cerebrospinal fluid 25% and THP-1 cell culture supernatant 50%. The interpolated dilution factor corrected values (to neat samples) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)

Standard curve comparison between the Chemiluminescent Human TREM2 SimpleStep ELISA and colorimetric Human TREM2 SimpleStep ELISA (ab224881) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)

Interpolated concentrations of native TREM2 in human serum and plasma samples. The concentrations of TREM2 were measured in duplicates, interpolated from the TREM2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5%, and plasma (citrate) 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)

Standard curve comparison between the Chemiluminescent Human TREM2 SimpleStep ELISA and colorimetric Human TREM2 SimpleStep ELISA (ab224881) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean TREM2 concentration was determined to be 30 ng/mL with a range of 13 – 66 ng/mL.

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)
  • sELISA

Lab

Sandwich ELISA - Human TREM2 ELISA Kit, Chemiluminescent (AB325449)

Dynamic range comparison between the Chemiluminescent Human TREM2 SimpleStep ELISA, colorimetric Human TREM2 SimpleStep ELISA (ab224881), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Cerebral Spinal Fluid, Cell Extract, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.24 pg/mL

Range

8.23 - 6000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human TREM2 ELISA Kit, Chemiluminescent (ab325449) is a rapid single-wash sandwich 55-min ELISA to measure Human TREM2 in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant, Cerebrospinal Fluid, Cell Extract, Tissue Extract with a sensitivity of 2.24 pg/ml.

How the assay works

Human TREM2 ELISA Kit, Chemiluminescent (ab325449) SimpleStep ELISA®  employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Please refer to our protocol booklet for more details.

Human TREM2 (ab325449) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TREM2 antibody [EPR20243-78] - BSA and Azide free (Capture) ab244673

- Anti-TREM2 antibody [EPR20243-269] - BSA and Azide free (Detector) ab244938

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.9" } ]
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Recovery

[ { "sample": "Serum", "range": "85 - 104 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "90 - 103 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "93 - 105 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "97 - 111 %", "average": "= 103" }, { "sample": "Cell culture supernatant", "range": "86 - 94 %", "average": "= 91" }, { "sample": "Cerebral Spinal Fluid", "range": "109 - 113 %", "average": "= 111" }, { "sample": "Cell Lysate", "range": "100 - 117 %", "average": "= 109" }, { "sample": "Tissue Extracts", "range": "95 - 101 %", "average": "= 109" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

The protein expressed by gene TREM2 forms a receptor signaling complex with TYROBP, facilitating signaling and cell activation upon ligand binding. It serves as a receptor for amyloid-beta protein 42, mediating its uptake and degradation by microglia, and also acts as a receptor for lipoprotein particles such as LDL, VLDL, HDL, and various apolipoproteins, enhancing their uptake in microglia. TREM2 binds phospholipids, preferably anionic lipids, and is necessary for microglial phagocytosis of apoptotic neurons. It regulates changes in microglial numbers with age and activates immune responses in macrophages and dendritic cells, where it plays a role in chemokine receptor CCR7 up-regulation, as well as dendritic cell maturation and survival. Additionally, TREM2 is involved in positively regulating osteoclast differentiation. This supplementary information is collated from multiple sources and compiled automatically.
See full target information TREM2
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com