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AB108780

Human Anti-Trichinella spiralis IgG ELISA Kit

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(1 Publication)

Human Anti-Trichinella spiralis IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Trichinella spiralis in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Trichinella spiralis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Trichinella spiralis in Human serum and plasma.

A 96-well plate has been precoated with Trichinella spiralis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Trichinella spiralis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Trichinella spiralis IgG sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108780-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Trichinella spiralis Protein A HRP Conjugate", "number":"AB108780-CMP08", "productcode":"" }, { "size":"1 x 2 mL", "name":"Trichinella spiralis IgG Positive Control", "number":"AB108780-CMP07", "productcode":"" }, { "size":"1 x 2 mL", "name":"Trichinella spiralis IgG Negative Control", "number":"AB108780-CMP06", "productcode":"" }, { "size":"1 x 3 mL", "name":"Trichinella spiralis IgG Cut-off Control", "number":"AB108780-CMP05", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgG Sample Diluent", "number":"AB108780-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108780-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108780-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108780-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108780-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108780-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Trichinella spiralis (IgG) Coated Microplate (12 x 8 wells)", "number":"AB108780-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Trichinella spiralis IgG is an important antibody for detecting Trichinella spiralis a parasitic nematode responsible for trichinellosis in humans. Alternative names include anti-Trichinella spiralis antibody. The IgG class of antibodies has a typical mass around 150 kDa as is common with most IgG antibodies. It is primarily expressed in the serum of infected individuals where it participates in immune responses against the parasite. As part of the host's adaptive immunity these antibodies bind to specific antigens on the surface of the Trichinella spiralis larvae marking them for destruction by immune cells.
Biological function summary

The role of Trichinella spiralis IgG involves recognition and neutralization of the parasitic nematodes in the host organism. While not part of a multi-protein complex itself IgG antibodies operate through a network of immune interactions enhancing phagocytosis by macrophages and neutrophils via opsonization. This immune response reduces parasitic loads and assists in containing the spread of infection within tissues such as muscle where the larvae encyst. As with other IgG antibodies they initiate complement activation further promoting parasite elimination.

Pathways

The activity of Trichinella spiralis IgG is integral to both the classical and the alternative complement pathways. This antibody interacts with the complement system proteins such as C1q which start the cascade leading to the formation of the membrane attack complex. The regulation of humoral immune responses involves Fc receptors on various immune cells which recognize the Fc region of IgG. Proteins like C4 and C3 in the complement pathway relate with the functional activities driven by these antibodies facilitating pathogen lysis and clearance.

Trichinella spiralis IgG antibodies are primarily associated with the diagnosis and progression of trichinellosis characterized by gastrointestinal disturbances and muscle pain. During infection the body's immune response to the parasite can cause inflammation and tissue damage leading to symptoms. Interestingly cross-reactivity with other helminth infections might occur due to shared antigenic features which could involve proteins like helminth-derived proteases. Accurate detection of specific IgG antibodies helps in confirming diagnosis and distinguishing trichinellosis from other parasitic infections that exhibit similar clinical signs.
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS neglected tropical diseases 15:e0009723 PubMed34432796

2021

Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Ning Xu,Yansong Li,Bin Tang,Hualin Yang,Weihua Gao,Mingyuan Liu,Xiaolei Liu,Yu Zhou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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