JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB326013

Human TROP2 ELISA Kit, Chemiluminescent- Extracellular

Be the first to review this product! Submit a review

|

(0 Publication)

Human TROP2 ELISA Kit, Chemiluminescent- Extracellular is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human TROP2 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 1.83 - 4000 pg/ml with a sensitivity of 0.41 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

GA733-1, M1S1, TROP2, TACSTD2, Tumor-associated calcium signal transducer 2, Cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1, Pancreatic carcinoma marker protein GA733-1

6 Images
Sandwich ELISA - Human TROP2 ELISA Kit, Chemiluminescent- Extracellular (AB326013)
  • sELISA

Lab

Sandwich ELISA - Human TROP2 ELISA Kit, Chemiluminescent- Extracellular (AB326013)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human TROP2 ELISA Kit, Chemiluminescent- Extracellular (AB326013)
  • sELISA

Supplier Data

Sandwich ELISA - Human TROP2 ELISA Kit, Chemiluminescent- Extracellular (AB326013)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TROP2 concentration was determined to be 116.15 pg/mL with a range of 88.9 – 140.6 pg/mL.

Sandwich ELISA - Human TROP2 ELISA Kit, Chemiluminescent- Extracellular (AB326013)
  • sELISA

Lab

Sandwich ELISA - Human TROP2 ELISA Kit, Chemiluminescent- Extracellular (AB326013)

Dynamic range comparison between the Chemiluminescent Human TROP2 SimpleStep ELISA, colorimetric Human TROP2 SimpleStep ELISA (ab322439), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human TROP2 ELISA Kit, Chemiluminescent- Extracellular (AB326013)
  • sELISA

Lab

Sandwich ELISA - Human TROP2 ELISA Kit, Chemiluminescent- Extracellular (AB326013)

Interpolated concentration of native TROP2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : A-431 cell culture supernatant 12.5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

Sandwich ELISA - Human TROP2 ELISA Kit, Chemiluminescent- Extracellular (AB326013)
  • sELISA

Lab

Sandwich ELISA - Human TROP2 ELISA Kit, Chemiluminescent- Extracellular (AB326013)

Interpolated concentration of native TROP2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 100%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

Sandwich ELISA - Human TROP2 ELISA Kit, Chemiluminescent- Extracellular (AB326013)
  • sELISA

Lab

Sandwich ELISA - Human TROP2 ELISA Kit, Chemiluminescent- Extracellular (AB326013)

Standard curve comparison between the Chemiluminescent Human TROP2 SimpleStep ELISA and colorimetric Human TROP2 SimpleStep ELISA (ab322439) in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.41 pg/mL

Range

1.83 - 4000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human TROP2 ELISA Kit, Chemiluminescent- Extracellular (ab326013) is a rapid single-wash sandwich 55-min ELISA to measure Human TROP2 in Serum, Citrate Plasma, EDTA Plasma, Cell Culture Supernatant with a sensitivity of 0.41 pg/mL.

How the assay works

Human TROP2 ELISA Kit, Chemiluminescent- Extracellular (ab326013) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human TROP2 (ab326013) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TROP2 antibody [EPR26737-78] - BSA and Aside free (Capture) ab315927
- Anti-TROP2 antibody [EPR26737-87] - BSA and Aside free (Detector) ab315928

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.4" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.5" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "86 - 97 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "104 - 109 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "86 - 113 %", "average": "= 100" }, { "sample": "Cell culture supernatant", "range": "88 - 93 %", "average": "= 91" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326013-1x96Tests", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326013-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"Cell Extraction Buffer SSW", "number":"AB326013-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TROP2 Lyophilized Recombinant Protein", "number":"AB326013-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB326013-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human TROP2 Capture Antibody", "number":"AB326013-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326013-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326013-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TROP2 Detector Antibody", "number":"AB326013-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326013-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326013-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326013-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

May function as a growth factor receptor.
See full target information TACSTD2
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com