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AB314712

Human Troponin C ELISA Kit

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Human Troponin C ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Troponin C in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

TNNC, TNNC1, TN-C

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Sandwich ELISA - Human Troponin C ELISA Kit (AB314712)
  • sELISA

Supplier Data

Sandwich ELISA - Human Troponin C ELISA Kit (AB314712)

Example of standard curve using ab314712. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.11 ng/mL

Range

0.11 - 75 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Troponin C ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Troponin C in serum (human Troponin C concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human Troponin C coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "94 - 108 %", "average": "= 136.7" }, { "sample": "Plasma", "range": "72 - 134 %", "average": "= 100.7" }, { "sample": "Cell culture media", "range": "96 - 148 %", "average": "= 122.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314712-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Biotinylated Human Troponin C Detection Antibody", "number":"AB314712-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314712-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314712-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Troponin C Protein Standard", "number":"AB314712-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Troponin C Antibody-coated ELISA Plate", "number":"AB314712-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314712-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB314712-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin", "number":"AB314712-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314712-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Troponin C often referred to as TnC is a protein that plays a mechanical role in muscle contraction. It is part of the troponin complex weighing approximately 18 kDa. This protein is expressed in cardiac and skeletal muscles where it functions as a calcium-binding component. Troponin C by responding to changes in calcium ion concentration interacts with troponin I and troponin T to regulate the contraction and relaxation cycles of muscle fibers.
Biological function summary

Through its calcium binding ability Troponin C initiates a conformational change in the troponin complex. This action releases the inhibition imposed by tropomyosin on actin filaments thereby allowing myosin heads to interact with actin. As part of the troponin complex Troponin C ensures precise muscle function and is essential for cardiac and skeletal muscle physiology. Its interaction with troponin I and troponin T highlights its regulatory role in muscle contraction.

Pathways

The protein is integral to the muscle contraction pathway and cardiac function. Within these pathways Troponin C's role is connected to the sliding filament model of muscle contraction where it regulates actin-myosin interactions. It works closely with proteins like myosin and its function is tied to multiple signaling pathways like the calcium signaling pathway which further influences muscle contraction and relaxation cycles.

Troponin C is highly significant in the context of cardiovascular diseases and skeletal muscle disorders. Elevated levels of the troponin complex in the bloodstream often indicate conditions such as myocardial infarction. Moreover mutations affecting Troponin C can lead to cardiomyopathies including hypertrophic and dilated cardiomyopathy. Its relation to troponin I and troponin T within these disorders emphasizes the critical nature of protein interactions in maintaining muscle health and identifying disease.
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Product protocols

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Target data

Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components : Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
See full target information TNNC1
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websiteProtocolBooklet
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Complementary Products

Proteins & Peptides

AB86690

Recombinant Human Troponin C protein

SDS-PAGE - Recombinant Human Troponin C protein (AB86690)

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