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AB317772

Human Trypsin 1 ELISA Kit

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Human Trypsin 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Trypsin 1 with a sensitivity of 48.227 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

TRP1, TRY1, TRYP1, PRSS1, Serine protease 1, Anionic trypsin I, Anionic trypsin-I, Beta-trypsin, Cationic trypsinogen, Pretrypsinogen I, Trypsin I, Trypsin-1

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Sandwich ELISA - Human Trypsin 1 ELISA Kit (AB317772)
  • sELISA

Supplier Data

Sandwich ELISA - Human Trypsin 1 ELISA Kit (AB317772)

Interpolated concentrations of Trypsin 1 in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Trypsin 1 concentration was determined to be 93.5 ng/mL with a range of 29.6 – 173.4 ng/mL.

Sandwich ELISA - Human Trypsin 1 ELISA Kit (AB317772)
  • sELISA

Supplier Data

Sandwich ELISA - Human Trypsin 1 ELISA Kit (AB317772)

Interpolated concentrations of human Trypsin 1 in serum in 96-well vs. 384-well plates. Interpolated concentration of native Trypsin 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 20 serum.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Trypsin 1 ELISA Kit (AB317772)
  • sELISA

Supplier Data

Sandwich ELISA - Human Trypsin 1 ELISA Kit (AB317772)

Example of human Trypsin 1 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Trypsin 1 ELISA Kit (AB317772)
  • sELISA

Supplier Data

Sandwich ELISA - Human Trypsin 1 ELISA Kit (AB317772)

Interpolated concentrations of human Trypsin 1 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native Trypsin 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1 : 20 serum, 1 : 20 plasma (citrate), 1 : 40 plasma (EDTA), and 1 : 20 plasma (heparin).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Trypsin 1 ELISA Kit (AB317772)
  • sELISA

Supplier Data

Sandwich ELISA - Human Trypsin 1 ELISA Kit (AB317772)

Example of human Trypsin 1 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Cell culture media, Citrate plasma, Heparin Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 48.227 pg/mL

Range

140.625 - 9000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

AB200997

Anti-Trypsin antibody [EPR19498-43]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trypsin antibody [EPR19498-43] (AB200997)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Trypsin 1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Trypsin 1 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Human Trypsin 1 with 48.227 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.7" } ]
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Recovery

[ { "sample": "Serum", "range": "99 - 110 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "92 - 103 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "95 - 102 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "114 - 118 %", "average": "= 116" }, { "sample": "Cell culture media", "range": "76 - 83 %", "average": "= 80" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Trypsin is a serine protease enzyme with a molecular mass around 24 kDa. Known also as "bovine trypsin" or "pancreatic trypsin" it functions primarily in the digestive system. Trypsin hydrolyzes peptide bonds specifically at the carboxyl side of the amino acids lysine and arginine. The enzyme is produced in the pancreas as an inactive precursor trypsinogen which converts to active trypsin in the small intestine.
Biological function summary

Trypsin breaks down proteins into smaller peptides facilitating digestion. It is not part of a complex but interacts with other pancreatic enzymes like chymotrypsin and elastase. Trypsin also plays a regulatory role in activating other zymogens like procarboxypeptidase and chymotrypsinogen essential in the complete protein digestion process.

Pathways

The catalytic actions of trypsin are integral to the digestive pathway in the gastrointestinal tract. It ensures the efficient breakdown and absorption of nutrients. In the trypsin activation pathway it activates other proteases which are significant for protein catabolism. This enzyme collaborates closely with anti-trypsin inhibitors that regulate its activity preventing autodigestion and maintaining homeostasis.

Trypsin relates closely to pancreatitis and cystic fibrosis. Unregulated activation of trypsinogen within the pancreas causes pancreatitis a condition characterized by inflammation and damage to pancreatic cells. Alpha-1 anti-trypsin an important inhibitor of trypsin connects to conditions like emphysema and liver disease due to its dysfunctional regulation indicating a broader implication of trypsin in maintaining cellular health.
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Product protocols

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Target data

Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
See full target information PRSS1
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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