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AB267611

Human Trypsin 2 ELISA Kit

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Human Trypsin 2 ELISA Kit is a Sandwich ELISA for the measurement of Human Trypsin 2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

TRY2, TRYP2, PRSS2, Trypsin-2, Anionic trypsinogen, Serine protease 2, Trypsin II

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Sandwich ELISA - Human Trypsin 2 ELISA Kit (AB267611)
  • sELISA

Supplier Data

Sandwich ELISA - Human Trypsin 2 ELISA Kit (AB267611)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 25 pg/mL

Range

24.58 - 6000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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  • 5
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Primary Antibodies

AB166898

Anti-Trypsinogen antibody [EPR11665(B)]

RabMAb|Recombinant

Western blot - Anti-Trypsinogen antibody [EPR11665(B)] (AB166898)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Trypsin 2 ELISA Kit is designed for the quantitative determination of Trypsin 2 in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human Trypsin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Trypsin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Trypsin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Trypsin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "97 - 142 %", "average": "= 119.2" }, { "sample": "Plasma", "range": "86 - 143 %", "average": "= 114.7" }, { "sample": "Cell culture media", "range": "96 - 104 %", "average": "= 99.72" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267611-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267611-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267611-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267611-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB267611-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Trypsin 2 Standard (Lyophilized)", "number":"AB267611-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Trypsin 2 Detection Antibody", "number":"AB267611-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB267611-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Trypsin 2 coated Microplate (12 x 8 wells)", "number":"AB267611-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267611-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

In the ileum, may be involved in defensin processing, including DEFA5.
See full target information PRSS2
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com