Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Tryptase alpha/beta-1 (TPSAB1) in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 12.5 - 800 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 3.2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed:18854315).
Alternative names
TPS1, TPS2, TPSB1, TPSAB1, Tryptase alpha/beta-1, Tryptase-1, Tryptase I, Tryptase alpha-1
What's included?
Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Tryptase alpha/beta-1 (TPSAB1) in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 12.5 - 800 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3.2 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. = 3.2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. = 3.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 106 | Range 98 - 113 % |
Sample type Citrate plasma | Average % = 100 | Range 97 - 103 % |
Sample type EDTA Plasma | Average % = 118 | Range 113 - 124 % |
Sample type Heparin Plasma | Average % = 97 | Range 91 - 108 % |
Sample type Cell culture supernatant | Average % = 99 | Range 98 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Tryptase alpha/beta-1 (TPSAB1) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tryptase alpha/beta-1 (TPSAB1) protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Tryptase alpha/beta-1 (TPSAB1) with 3.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
Singlewash protocol reduces assay time to 90 minutes or less.
High sensitivity, specificity and reproducibility from superior antibodies.
Fully validated in biological samples.
96wells plate breakable into 12 x 8 wells strips, available in 20pack (10 x 96well plates).
Also available in a fully validated 384well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (ab323931)
Interpolated concentration of native Tryptase alpha/beta-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (ab323931)
Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Tryptase alpha/beta-1 concentration was determined to be 1,994 pg/mL with a range of 553 – 3,713 pg/mL.
Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (ab323931)
Interpolated concentration of native Tryptase alpha/beta-1 was measured in duplicate at different sample concentrations. Undiluted samples are 100% stimulated PBMC supernatant (1.5% PHA-M for 46 hrs) and unstimulated PBMC supernatant (mock treated). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (ab323931)
Interpolated concentration of native Tryptase alpha/beta-1 was measured in duplicate at different sample concentrations. Undiluted samples are 25% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (ab323931)
Example of human Tryptase alpha/beta-1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit (ab323931)
Example of human Tryptase alpha/beta-1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
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