AB223594

Human TSC-1 ELISA Kit

Recombinant
SimpleStep
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Human TSC-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TSC-1 with a sensitivity of 1.54 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

SCYA26, UNQ216/PRO242, CCL26, C-C motif chemokine 26, CC chemokine IMAC, Eotaxin-3, Macrophage inflammatory protein 4-alpha, Small-inducible cytokine A26, Thymic stroma chemokine-1, MIP-4-alpha, TSC-1

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Sandwich ELISA - Human TSC-1 ELISA Kit (AB223594)

sELISA

Supplier Data

Sandwich ELISA - Human TSC-1 ELISA Kit (AB223594)

Interpolated concentrations of native Eotaxin 3 in human A549 cell culture supernatant samples.

The concentrations of Eotaxin 3 were measured in duplicates, interpolated from the Eotaxin 3 standard curves and corrected for sample dilution. Undiluted human A549 supernatant : 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin 3 concentration was determined to be 5559.17 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human TSC-1 ELISA Kit (AB223594)

Interpolated concentrations of spiked Eotaxin 3 in serum and plasma.

Interpolated concentrations of spiked Eotaxin 3 in human serum 50%, plasma (citrate) 50% and plasma (EDTA) 50%. The concentrations of Eotaxin 3 were measured in duplicate and interpolated from the Eotaxin 3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin 3 concentration was determined to be 161 pg/mL in human serum and 320.02 pg/mL in citrate plasma, 318.05 pg/ml in EDTA plasma.

sELISA

Supplier Data

Sandwich ELISA - Human TSC-1 ELISA Kit (AB223594)

Example of Human Eotaxin 3 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.54 pg/mL

Range

4.69 - 300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human TSC-1 ELISA Kit (ab223594) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TSC-1 protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TSC-1 with 1.54 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

TSC-1 (Eotaxin 3 / CCL26) is a small cytokine within the CC chemokine family coded by a gene located on human chromosome 7. TSC-1 is also know by Macrophage inflammatory protein 4-alpha, Thymic stroma chemokine-1 and IMAC. It has known expression in heart, lung and ovarian tissue. TSC-1 expression can be induced by IL-4 stimulation which then can activate chemotaxic activity of eosinophils and basophiles by binding to the cell surface chemokine receptor CCR3.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.7" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "95 - 108 %", "average": "= 101" }, { "sample": "Serum", "range": "90 - 118 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "85 - 109 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "83 - 117 %", "average": "= 102" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tuberous Sclerosis Complex 1 (TSC-1) also known as hamartin is a protein with a molecular mass of approximately 130 kDa. It plays a direct role in cellular signaling and regulatory functions. TSC-1 is expressed broadly across tissues which suggests its importance in maintaining normal cellular physiology. It interfaces with various signaling molecules and directly interacts with TSC-2 to form a protein complex.

Biological function summary

TSC-1 plays a regulatory role in cell growth and proliferation. It forms a complex with TSC-2 also known as tuberin which is important for its function. This complex acts as a GAP (GTPase-activating protein) toward Rheb a small GTPase inhibiting the mTOR signaling pathway. The proper regulation of this pathway ensures controlled cell size and growth emphasizing the importance of the TSC-1/TSC-2 complex in cellular regulation.

Pathways

TSC-1 operates within the mTOR signaling pathway to influence cellular processes like autophagy and protein synthesis. It also relates to the PI3K/AKT pathway one that is involved in the regulation of cell survival and metabolism. By interacting with TSC-2 in these pathways TSC-1 modulates the inhibition of mTORC1 thereby impacting cellular homeostasis and growth.

Defects in TSC-1 can lead to tuberous sclerosis complex a condition characterized by benign tumors in multiple organs. Mutations in TSC-1 and its partner TSC-2 disrupt normal cellular functions leading to this disease. Additionally aberrations in the mTOR pathway influenced by TSC-1 malfunction have associations with certain cancers highlighting the importance of TSC-1 in maintaining cell proliferation and signaling balance.

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Product protocols

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Target data

Chemoattractant for eosinophils and basophils (PubMed : 10415065, PubMed : 10488147). Acts as a ligand for C-C chemokine receptor CCR3 which triggers Ca(2+) mobilization in eosinophils (PubMed : 10415065, PubMed : 10488147, PubMed : 11425309). Also acts as a ligand for CX3C chemokine receptor CX3CR1, inducing cell chemotaxis (PubMed : 20974991).

See full target information CCL26

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human TSC-1 ELISA Kit

Sandwich ELISA - Human TSC-1 ELISA Kit (AB223594)

Sandwich ELISA - Human TSC-1 ELISA Kit (AB223594)

Sandwich ELISA - Human TSC-1 ELISA Kit (AB223594)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise