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AB155444

Human TSLP ELISA Kit

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(2 Publications)

Human TSLP ELISA Kit is a Sandwich ELISA for the measurement of Human TSLP in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Thymic stromal lymphopoietin, TSLP

2 Images
Sandwich ELISA - Human TSLP ELISA Kit (AB155444)
  • sELISA

Unknown

Sandwich ELISA - Human TSLP ELISA Kit (AB155444)

Representative standard curve using ab155444 - Assay Diluent D.

Sandwich ELISA - Human TSLP ELISA Kit (AB155444)
  • sELISA

Unknown

Sandwich ELISA - Human TSLP ELISA Kit (AB155444)

Representative standard curve using ab155444 - Assay Diluent B.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 3 pg/mL

Range

3.3 - 800 pg/mL

Assay time

5h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TSLP Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed the quantitative measurement of Human TSLP in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human TSLP coated on a 96-well plate. Standards and samples are pipetted into the wells and TSLP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TSLP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TSLP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with Human TSLP ELISA Kit (ab192149) from our SimpleStep ELISA® range.

Optimization may be required with urine samples.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "100 - 129 %", "average": "= 117.6" }, { "sample": "Plasma", "range": "120 - 139 %", "average": "= 130.7" }, { "sample": "Cell culture media", "range": "70 - 90 %", "average": "= 85.21" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155444-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155444-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155444-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"TSLP Standard Human (recombinant) (lyophilized)", "number":"AB155444-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155444-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB155444-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TSLP-coated Microplate", "number":"AB155444-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155444-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Detection Antibody TSLP : biotinylated anti-human TSLP", "number":"AB155444-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB155444-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSLP or thymic stromal lymphopoietin is a cytokine protein weighing around 27 kDa. It is also known as a hematopoietic cytokine and functions in the signaling processes of the immune system. TSLP is expressed in epithelial cells most notably in the skin lungs and gut. This cytokine holds significance due to its role in immune responses acting through its receptor complex made of TSLP receptor (TSLPR) and the interleukin-7 receptor alpha chain (IL-7Rα).
Biological function summary

TSLP plays a role in mediating immune system responses. It induces the differentiation of T-helper 2 (Th2) cells promoting a specific type of immune response. TSLP does not appear to be part of a larger protein complex but it often functions in association with the TSLPR and IL-7Rα receptor complex. It influences the activity and maturation of dendritic cells which are important for initiating immune responses.

Pathways

A mechanical understanding of TSLP reveals its involvement in both the JAK-STAT and NF-kB signaling pathways. It interacts with proteins like JAK2 and STAT5 which are important for the downstream signaling cascade leading to inflammation and immune responses. These pathways play important roles in the immune system's ability to detect and respond to environmental triggers.

TSLP has relevance in conditions like asthma and atopic dermatitis. It promotes inflammation that contributes to these allergic diseases. The dysregulation of TSLP expression can exacerbate these conditions. In asthma TSLP interacts with proteins such as interleukin-33 (IL-33) to enhance inflammatory responses in airway tissues. In atopic dermatitis connections with interleukin-4 (IL-4) are noted as both cytokines contribute to the inflammatory processes involved in the disease pathology. These associations make TSLP a potential target for therapeutic interventions in such disorders.
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Product protocols

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Target data

Isoform 1. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.. Isoform 2. May act as an antimicrobial peptide in the oral cavity and on the skin.
See full target information TSLP
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of pharmacology and experimental therapeutics 384:248-253 PubMed36351795

2022

Human Induced Pluripotent Stem Cell-Derived Keratinocyte-Like Cells for Research on Protease-Activated Receptor 2 in Nonhistaminergic Cascades of Atopic Dermatitis .

Applications

Unspecified application

Species

Unspecified reactive species

Rio Nishimoto,Chinatsu Kodama,Hiromi Yamashita,Fumiyuki Hattori

BMC pulmonary medicine 19:66 PubMed30890137

2019

Elevated serum OX40L is a biomarker for identifying corticosteroid resistance in pediatric asthmatic patients.

Applications

Unspecified application

Species

Unspecified reactive species

Su-Li Ma,Lei Zhang
View all publications
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websiteProtocolBooklet
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Product promise

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