Human TTK/Mps1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human TTK/Mps1 in Human in Cell culture supernatant, Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1 - 240 ng/mL
- Sensitivity
- = 1 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in mitotic spindle assembly checkpoint signaling, a process that delays anaphase until chromosomes are bioriented on the spindle, and in the repair of incorrect mitotic kinetochore-spindle microtubule attachments (PubMed:18243099, PubMed:28441529, PubMed:29162720). Phosphorylates MAD1L1 to promote the mitotic spindle assembly checkpoint (PubMed:18243099, PubMed:29162720). Phosphorylates CDCA8/Borealin leading to enhanced AURKB activity at the kinetochore (PubMed:18243099). Phosphorylates SKA3 at 'Ser-34' leading to dissociation of the SKA complex from microtubules and destabilization of microtubule-kinetochore attachments (PubMed:28441529). Phosphorylates KNL1, KNTC1 and autophosphorylates (PubMed:28441529).
Alternative names
MPS1, MPS1L1, TTK, Dual specificity protein kinase TTK, Phosphotyrosine picked threonine-protein kinase, PYT
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Human TTK/Mps1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human TTK/Mps1 in Human in Cell culture supernatant, Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1 - 240 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 82.98 | Range 76 - 88 % |
Sample type Plasma | Average % = 82.43 | Range 73 - 99 % |
Sample type Cell culture media | Average % = 92.79 | Range 77 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human TTK/Mps1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TTK/Mps1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human TTK/Mps1 coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TTK also known as Mps1 is a dual-specificity protein kinase with a molecular mass of approximately 97 kDa. It plays a mechanical role in the regulation of the cell cycle by phosphorylating various substrates required for accurate chromosome alignment and segregation during mitosis. TTK/Mps1 is expressed in many tissues but shows the highest levels in rapidly dividing cells such as in the testis and in tumors. The presence of this protein in proliferative tissues suggests its involvement in cell cycle control mechanisms.
- Biological function summary
TTK/Mps1 protein is essential for maintaining genomic stability. It functions as part of the mitotic checkpoint complex (MCC) which prevents cells from prematurely advancing from metaphase to anaphase until all chromosomes are correctly aligned on the mitotic spindle. By doing so TTK/Mps1 ensures proper chromosomal attachment and tension at kinetochores reducing the likelihood of aneuploidy. This regulation highlights its critical role in preventing genetic variation during cell division.
- Pathways
TTK/Mps1 is an important component of the spindle assembly checkpoint (SAC) pathway which plays an important role in cell cycle regulation. It works closely with other proteins such as BUBR1 BUB3 and MAD2 to halt cell cycle progression until chromosomal alignment is verified. In addition TTK/Mps1 interacts with the aurora kinase A (AURKA) pathway which is instrumental in centrosome maturation and spindle assembly further emphasizing its role in ensuring accurate mitosis.
- Associated diseases and disorders
Aberrations in TTK/Mps1 activity are associated with cancer and aneuploidy. Overexpression or amplification of TTK/Mps1 is frequently observed in various tumors linking it with cancer progression and poor prognosis. Its interaction with other proteins like p53 a well-known tumor suppressor is significant in understanding its role in oncogenesis. Therapeutic targeting of TTK/Mps1 is being explored to exploit its critical function in rapidly proliferating cancer cells aiming to induce mitotic catastrophe and mitigate tumor growth.
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1 product image
Sandwich ELISA - Human TTK/Mps1 ELISA Kit (ab314716)
Example of standard curve using ab314716. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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