JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314716

Human TTK/Mps1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human TTK/Mps1 ELISA Kit is a Sandwich ELISA for the measurement of Human TTK/Mps1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

MPS1, MPS1L1, TTK, Dual specificity protein kinase TTK, Phosphotyrosine picked threonine-protein kinase, PYT

1 Images
Sandwich ELISA - Human TTK/Mps1 ELISA Kit (AB314716)
  • sELISA

Supplier Data

Sandwich ELISA - Human TTK/Mps1 ELISA Kit (AB314716)

Example of standard curve using ab314716. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1 ng/mL

Range

1 - 240 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB11108

Anti-TTK/Mps1 antibody [N1] - BSA free

Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (AB11108)

  • 4
  • 8
Proteins & Peptides

AB89589

Recombinant human TTK/Mps1 protein

Functional Studies - Recombinant human TTK/Mps1 protein (AB89589)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human TTK/Mps1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TTK/Mps1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human TTK/Mps1 coated on a 96-well plate.
style
left-column

Recovery

[ { "sample": "Serum", "range": "76 - 88 %", "average": "= 82.98" }, { "sample": "Plasma", "range": "73 - 99 %", "average": "= 82.43" }, { "sample": "Cell culture media", "range": "77 - 101 %", "average": "= 92.79" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314716-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314716-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB314716-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human TTK/Mps1 Detection Antibody", "number":"AB314716-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314716-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human TTK/Mps1 Protein Standard", "number":"AB314716-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314716-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314716-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human TTK/Mps1 Antibody-coated ELISA Plate", "number":"AB314716-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTK also known as Mps1 is a dual-specificity protein kinase with a molecular mass of approximately 97 kDa. It plays a mechanical role in the regulation of the cell cycle by phosphorylating various substrates required for accurate chromosome alignment and segregation during mitosis. TTK/Mps1 is expressed in many tissues but shows the highest levels in rapidly dividing cells such as in the testis and in tumors. The presence of this protein in proliferative tissues suggests its involvement in cell cycle control mechanisms.
Biological function summary

TTK/Mps1 protein is essential for maintaining genomic stability. It functions as part of the mitotic checkpoint complex (MCC) which prevents cells from prematurely advancing from metaphase to anaphase until all chromosomes are correctly aligned on the mitotic spindle. By doing so TTK/Mps1 ensures proper chromosomal attachment and tension at kinetochores reducing the likelihood of aneuploidy. This regulation highlights its critical role in preventing genetic variation during cell division.

Pathways

TTK/Mps1 is an important component of the spindle assembly checkpoint (SAC) pathway which plays an important role in cell cycle regulation. It works closely with other proteins such as BUBR1 BUB3 and MAD2 to halt cell cycle progression until chromosomal alignment is verified. In addition TTK/Mps1 interacts with the aurora kinase A (AURKA) pathway which is instrumental in centrosome maturation and spindle assembly further emphasizing its role in ensuring accurate mitosis.

Aberrations in TTK/Mps1 activity are associated with cancer and aneuploidy. Overexpression or amplification of TTK/Mps1 is frequently observed in various tumors linking it with cancer progression and poor prognosis. Its interaction with other proteins like p53 a well-known tumor suppressor is significant in understanding its role in oncogenesis. Therapeutic targeting of TTK/Mps1 is being explored to exploit its critical function in rapidly proliferating cancer cells aiming to induce mitotic catastrophe and mitigate tumor growth.
style
left-column

Product protocols

style
left-column

Target data

Involved in mitotic spindle assembly checkpoint signaling, a process that delays anaphase until chromosomes are bioriented on the spindle, and in the repair of incorrect mitotic kinetochore-spindle microtubule attachments (PubMed : 18243099, PubMed : 28441529, PubMed : 29162720). Phosphorylates MAD1L1 to promote the mitotic spindle assembly checkpoint (PubMed : 18243099, PubMed : 29162720). Phosphorylates CDCA8/Borealin leading to enhanced AURKB activity at the kinetochore (PubMed : 18243099). Phosphorylates SKA3 at 'Ser-34' leading to dissociation of the SKA complex from microtubules and destabilization of microtubule-kinetochore attachments (PubMed : 28441529). Phosphorylates KNL1, KNTC1 and autophosphorylates (PubMed : 28441529). Phosphorylates MCRS1 which enhances recruitment of KIF2A to the minus end of spindle microtubules and promotes chromosome alignment (PubMed : 30785839).
See full target information TTK
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com