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AB324506

Human TWEAK/TNFSF12 ELISA Kit - Intracellular

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Human TWEAK/TNFSF12 ELISA Kit - Intracellular is a Sandwich (quantitative) ELISA for the measurement of Human TWEAK/TNFSF12 - Intracellular in Human in Cell/Tissue Extracts samples.

View Alternative Names

APO3L, DR3LG, UNQ181/PRO207, TNFSF12, Tumor necrosis factor ligand superfamily member 12, APO3 ligand, TNF-related weak inducer of apoptosis, TWEAK

4 Images
Sandwich ELISA - Human TWEAK/TNFSF12 ELISA Kit - Intracellular (AB324506)
  • sELISA

Lab

Sandwich ELISA - Human TWEAK/TNFSF12 ELISA Kit - Intracellular (AB324506)

Interpolated concentration of native TWEAK/TNFSF12 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 500 µg/mL human heart tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human TWEAK/TNFSF12 ELISA Kit - Intracellular (AB324506)
  • sELISA

Lab

Sandwich ELISA - Human TWEAK/TNFSF12 ELISA Kit - Intracellular (AB324506)

Interpolated concentration of native TWEAK/TNFSF12 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human heart tissue extract 500 µg/mL and human skeletal muscle tissue extract 1,000 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human TWEAK/TNFSF12 ELISA Kit - Intracellular (AB324506)
  • sELISA

Lab

Sandwich ELISA - Human TWEAK/TNFSF12 ELISA Kit - Intracellular (AB324506)

Example of human TWEAK/TNFSF12 standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TWEAK/TNFSF12 ELISA Kit - Intracellular (AB324506)
  • sELISA

Lab

Sandwich ELISA - Human TWEAK/TNFSF12 ELISA Kit - Intracellular (AB324506)

Example of human TWEAK/TNFSF12 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.5 pg/mL

Range

8.984 - 575 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TWEAK/TNFSF12 ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human TWEAK/TNFSF12 with a sensitivity of 1.5pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

-Design your own immunoassay: we also offer the conjugation-ready antibody pair.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.8" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.3" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "84 - 87 %", "average": "= 86" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.
See full target information TNFSF12
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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