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AB316897

Human TYK2 ELISA Kit

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Human TYK2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TYK2 with a sensitivity of 0.3 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Non-receptor tyrosine-protein kinase TYK2, TYK2

2 Images
Sandwich ELISA - Human TYK2 ELISA Kit (AB316897)
  • sELISA

Supplier Data

Sandwich ELISA - Human TYK2 ELISA Kit (AB316897)

Example of human TYK2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human TYK2 ELISA Kit (AB316897)
  • sELISA

Supplier Data

Sandwich ELISA - Human TYK2 ELISA Kit (AB316897)

Interpolated concentration of native TYK2 was measured in duplicate at different sample concentrations. Undiluted samples are 1,000 µg/mL Jurkat and MCF-7 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.3 ng/mL

Range

1.875 - 120 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human TYK2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TYK2 protein in Cell Lysate, Tissue extracts. Quantitate Human TYK2 with 0.3 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "110 - 112 %", "average": "= 111" }, { "sample": "Tissue Extracts", "range": "96 - 101 %", "average": "= 98" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab316897-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB316897-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB316897-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB316897-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316897-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB316897-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human TYK2 Lyophilized Recombinant Protein", "number":"AB316897-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TYK2 Capture Antibody", "number":"AB316897-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human TYK2 Detector Antibody", "number":"AB316897-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB316897-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316897-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB316897-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tyrosine kinase 2 (TYK2) also known as JAK1B is a non-receptor tyrosine kinase that weighs approximately 134 kDa. It belongs to the Janus kinase (JAK) family which includes JAK1 JAK2 and JAK3. TYK2 is vital in signal transduction for several cytokine receptors. The expression of TYK2 occurs broadly in different tissues including immune system cells. It plays an important mechanical role in transferring signals from cell surface receptors to intracellular signaling pathways which modulates various physiological processes.
Biological function summary

TYK2 mediates intracellular signaling that affects both innate and adaptive immune responses. It associates with cytokine receptors forming functional complexes that are critical for initiating JAK-STAT signaling. This type of signaling is responsible for regulating gene expression after cytokine binding. TYK2 is known for interacting with Type I interferons and interleukin-10 family cytokines indicating its importance in immune regulation.

Pathways

TYK2 acts within the JAK-STAT pathway a fundamental signaling cascade triggered by various cytokines and growth factors. The JAK-STAT pathway includes key partners such as STAT1 STAT2 and STAT3 proteins which are phosphorylated by TYK2 to propagate signal transduction. Additionally TYK2 is involved in the 'intercept blocking buffer' mechanism that adjusts signal strength thereby ensuring appropriate cellular responses to external stimuli.

Altered TYK2 activity has associations with immune-related conditions like psoriasis and systemic lupus erythematosus. Dysregulation of TYK2 can lead to improper immune signaling which contributes to these autoimmune conditions. Furthermore TYK2 interacts with other Janus kinase family members including JAK1 and JAK3 which can influence its role in the pathogenesis of these diseases potentially offering targets for therapeutic intervention.
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Product protocols

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Target data

Tyrosine kinase of the non-receptor type involved in numerous cytokines and interferons signaling, which regulates cell growth, development, cell migration, innate and adaptive immunity (PubMed : 10542297, PubMed : 10995743, PubMed : 7657660, PubMed : 7813427, PubMed : 8232552). Plays both structural and catalytic roles in numerous interleukins and interferons (IFN-alpha/beta) signaling (PubMed : 10542297). Associates with heterodimeric cytokine receptor complexes and activates STAT family members including STAT1, STAT3, STAT4 or STAT6 (PubMed : 10542297, PubMed : 7638186). The heterodimeric cytokine receptor complexes are composed of (1) a TYK2-associated receptor chain (IFNAR1, IL12RB1, IL10RB or IL13RA1), and (2) a second receptor chain associated either with JAK1 or JAK2 (PubMed : 10542297, PubMed : 25762719, PubMed : 7526154, PubMed : 7813427). In response to cytokine-binding to receptors, phosphorylates and activates receptors (IFNAR1, IL12RB1, IL10RB or IL13RA1), creating docking sites for STAT members (PubMed : 7526154, PubMed : 7657660). In turn, recruited STATs are phosphorylated by TYK2 (or JAK1/JAK2 on the second receptor chain), form homo- and heterodimers, translocate to the nucleus, and regulate cytokine/growth factor responsive genes (PubMed : 10542297, PubMed : 25762719, PubMed : 7657660). Negatively regulates STAT3 activity by promototing phosphorylation at a specific tyrosine that differs from the site used for signaling (PubMed : 29162862).
See full target information TYK2
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websiteProtocolBooklet
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