Human Ubiquitin+1 (UBB+1) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Ubiquitin+1 (UBB+1) with a sensitivity of 2.472 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 9.375 - 600 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2.472 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Ubiquitin. Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Alternative names
Polyubiquitin-B, UBB
What's included?
Recommended products
Human Ubiquitin+1 (UBB+1) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Ubiquitin+1 (UBB+1) with a sensitivity of 2.472 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 9.375 - 600 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2.472 pg/mL
- General recovery
- 89%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 6.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 89 | Range 82 - 95 % |
Sample type Citrate plasma | Average % = 97 | Range 86 - 115 % |
Sample type EDTA Plasma | Average % = 92 | Range 85 - 96 % |
Sample type Heparin Plasma | Average % = 96 | Range 92 - 102 % |
Sample type Cell culture media | Average % = 92 | Range 86 - 95 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Ubiquitin+1 (UBB+1) SimpleStep ELISA® kit (ab303754) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Ubiquitin+1 (UBB+1) protein in serum, plasma (hep), plasma (cit), plasma (EDTA) and cell culture media. Quantitate Human Ubiquitin+1 (UBB+1) with 2.472 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ubiquitin+1 (UBB+1) is an altered form of the ubiquitin protein which plays a significant role in protein degradation. This mutant ubiquitin results from a molecular error during transcription known as a frameshift mutation. The mass of UBB+1 is slightly greater than that of the normal ubiquitin due to an extra amino acid. UBB+1 is expressed primarily in neuronal cells where it accumulates due to its impaired function in tagging proteins for degradation by the proteasome.
- Biological function summary
The alteration in UBB+1 affects the cellular protein quality control system. UBB+1 cannot be recognized properly for degradation itself and hinders the ubiquitin-proteasome system (UPS) by competing with normal ubiquitin. UBB+1 does not just influence single proteins; it might also intervene in larger complexes related to proteostasis. Its aberrant accumulation disrupts the clearance of damaged or misfolded proteins which can impact cellular health.
- Pathways
Ubiquitin+1 affects several fundamental cellular processes by disturbing the UPS pathway. UBB+1 interacts with proteins involved in the ubiquitin-proteasome pathway such as E1 activating enzymes E2 conjugating enzymes and E3 ligases. Through these associations UBB+1 can induce stress in protein turnover and impair cellular regulation with direct implications in the processes related to protein homeostasis. The effect on these pathways can lead to wider disruptions in cellular signaling and maintenance.
- Associated diseases and disorders
Ubiquitin+1 has a notable impact on neurodegenerative diseases particularly Alzheimer's disease. It is associated with the accumulation of neurofibrillary tangles and plaques seen in these conditions. UBB+1 also connects with proteins like amyloid-beta which play central roles in Alzheimer's pathology. Furthermore UBB+1 accumulation has implications in Huntington’s disease where it links with the aggregation-prone mutant huntingtin protein. These associations highlight the significance of UBB+1 in disease mechanisms involving protein aggregation and cellular stress responses.
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3 product images
Sandwich ELISA - Human Ubiquitin+1 (UBB+1) ELISA Kit (ab303754)
Comparison of Ubiquitin+1 to Ubiquitin. Interpolated values (mean +/- SD) from a point within the detectable range of the assay are graphed. Mean interpolated values are as follows: Ubiquitin+1 295.94 pg/mL, Ubiquitin was not detected
Sandwich ELISA - Human Ubiquitin+1 (UBB+1) ELISA Kit (ab303754)
Example of human Ubiquitin+1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Ubiquitin+1 (UBB+1) ELISA Kit (ab303754)
Recombinant Ubiquitin+1 was spiked into the following species samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 50% in serum, plasma (citrate), plasma (EDTA), plasma (heparin), and 10F RPMI media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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