AB323602

Human UCHL1 ELISA Kit- Extracellular

Recombinant
SimpleStep
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Human UCHL1 ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human UCHL1 with a sensitivity of 7.379 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, PGP9.5, UCHL1

4 Images

Sandwich ELISA - Human UCHL1 ELISA Kit- Extracellular (AB323602)

sELISA

Supplier Data

Sandwich ELISA - Human UCHL1 ELISA Kit- Extracellular (AB323602)

Example of human UCHL1 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human UCHL1 ELISA Kit- Extracellular (AB323602)

Example of human UCHL1 standard curve in Sample Diluent NS + 1X Enhancer in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human UCHL1 ELISA Kit- Extracellular (AB323602)

Example of human UCHL1 standard curve in Sample Diluent NS and Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human UCHL1 ELISA Kit- Extracellular (AB323602)

Interpolated concentration of native UCHL1 was measured in duplicate at different sample concentrations. Undiluted samples are 50% cerebrospinal fluid. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 7.379 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human UCHL1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of UCHL1 protein in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cerebral Spinal Fluid. Quantitate Human UCHL1 with 7.379 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Cell culture media", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.2" }, { "reproducibilityType": "Inter", "sample": "Cell culture media", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.9" } ]

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Recovery

[ { "sample": "Serum", "range": "118 - 124 %", "average": "= 122" }, { "sample": "Citrate plasma", "range": "100 - 122 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "110 - 121 %", "average": "= 117" }, { "sample": "Heparin Plasma", "range": "114 - 119 %", "average": "= 117" }, { "sample": "Cell culture media", "range": "92 - 98 %", "average": "= 96" }, { "sample": "Cerebral Spinal Fluid", "range": "104 - 115 %", "average": "= 108" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab323602-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB323602-CMP09" }, { "size":"10 x 600 µL", "name":"10X Human UCHL1 Detector Antibody", "number":"AB323602-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human UCHL1 Capture Antibody", "number":"AB323602-CMP01" }, { "size":"20 x 1 Vial", "name":"Human UCHL1 Lyophilized Recombinant Protein", "number":"AB323602-CMP03" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB323602-CMP05" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323602-CMP06" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB323602-CMP11" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323602-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB323602-CMP08" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323602-CMP07" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB323602-CMP04" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab323602-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB323602-CMP09" }, { "size":"1 x 600 µL", "name":"10X Human UCHL1 Detector Antibody", "number":"AB323602-CMP02" }, { "size":"1 x 600 µL", "name":"10X Human UCHL1 Capture Antibody", "number":"AB323602-CMP01" }, { "size":"2 x 1 Vial", "name":"Human UCHL1 Lyophilized Recombinant Protein", "number":"AB323602-CMP03" }, { "size":"1 x 5 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB323602-CMP05" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323602-CMP06" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323602-CMP11" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB323602-CMP04" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB323602-CMP12" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323602-CMP07" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB323602-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab323602-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB323602-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human UCHL1 Detector Antibody", "number":"AB323602-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human UCHL1 Capture Antibody", "number":"AB323602-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human UCHL1 Lyophilized Recombinant Protein", "number":"AB323602-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB323602-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB323602-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB323602-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB323602-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB323602-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB323602-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB323602-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis (PubMed : 22212137, PubMed : 23359680). Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1 (PubMed : 22212137, PubMed : 25615526). In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways (PubMed : 12408865, PubMed : 8639624, PubMed : 9774100). Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production (PubMed : 22212137). Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes (By similarity). Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis (By similarity). Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators (By similarity). Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins (By similarity). Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation (PubMed : 25615526).

See full target information UCHL1

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websiteProtocolBooklet

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Product promise

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