AB288172

Human ULBP2 ELISA Kit

Recombinant
SimpleStep
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Human ULBP2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ULBP2 with a sensitivity of 6.18 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

N2DL2, RAET1H, UNQ463/PRO791, ULBP2, UL16-binding protein 2, ALCAN-alpha, NKG2D ligand 2, Retinoic acid early transcript 1H, N2DL-2, NKG2DL2

2 Images

Sandwich ELISA - Human ULBP2 ELISA Kit (AB288172)

sELISA

Supplier Data

Sandwich ELISA - Human ULBP2 ELISA Kit (AB288172)

Interpolated concentrations of human ULBP2 in A431 cell culture supernatant. Interpolated concentration of native ULBP2 was measured in duplicate at different sample concentrations. Undiluted samples are 25% A431 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human ULBP2 ELISA Kit (AB288172)

Example of human ULBP2 standard curve in Sample Diluent NS. Example of human ULBP2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 6.18 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human ULBP2 ELISA Kit (ab288172) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human ULBP2 protein in cell culture supernatant, plasma and serum samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ULBP2 with 6.18 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "97 - 125 %", "average": "= 107" }, { "sample": "Serum", "range": "65 - 91 %", "average": "= 80" }, { "sample": "EDTA Plasma", "range": "86 - 91 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "80 - 81 %", "average": "= 81" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ULBP2 also known as UL16-binding protein 2 and N2DL2 is a glycoprotein with a mass of approximately 30 kDa. It belongs to the family of NKG2D ligands and plays an essential role in the immune response. This protein is expressed on the surface of a variety of cells including epithelial cells fibroblasts and activated immune cells. Its expression can be induced by cellular stress infection or transformation making it an important player in the regulation of the immune system.

Biological function summary

The function of ULBP2 is to facilitate the activation of natural killer (NK) cells and cytotoxic T lymphocytes through binding to the NKG2D receptor. ULBP2 is not a part of a complex but acts independently to trigger immune responses. By interacting with NKG2D ULBP2 promotes the release of cytokines and enhances the cytolytic activity of these immune cells leading to the destruction of infected or transformed cells. This mechanism allows the immune system to efficiently target and eliminate compromised cells.

Pathways

The interaction between ULBP2 and the NKG2D receptor forms a critical part of the immune surveillance pathway. This pathway detects and responds to cellular abnormalities such as tumorigenesis or viral infections. ULBP2's activity relates closely with other NKG2D ligands and components of the immune checkpoint pathways notably involving proteins like MICA and MICB. Additionally ULBP2’s interaction impacts the balance between activating and inhibitory signals within the NK cell cascade thereby influencing immune system homeostasis.

ULBP2 expression has significant implications in cancer and autoimmune conditions. Elevated levels of ULBP2 are frequently associated with various types of cancer where they can either stimulate anti-tumor immunity or be shed to evade immune detection. In this context the protein interacts with NKG2D which is also expressed on tumor-infiltrating lymphocytes. Additionally altered expression of ULBP2 has been observed in inflammatory disorders potentially contributing to tissue damage due to sustained immune activation. Understanding these interactions is important for developing therapeutic strategies targeting ULBP2-related pathways.

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Product protocols

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Target data

Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.

See full target information ULBP2

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human ULBP2 ELISA Kit

Sandwich ELISA - Human ULBP2 ELISA Kit (AB288172)

Sandwich ELISA - Human ULBP2 ELISA Kit (AB288172)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Product promise