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AB267617

Human ULBP3 ELISA Kit

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Human ULBP3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human ULBP3 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

N2DL3, RAET1N, ULBP3, UL16-binding protein 3, ALCAN-gamma, NKG2D ligand 3, Retinoic acid early transcript 1N, N2DL-3, NKG2DL3

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Sandwich ELISA - Human ULBP3 ELISA Kit (AB267617)
  • sELISA

Supplier Data

Sandwich ELISA - Human ULBP3 ELISA Kit (AB267617)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.3 pg/mL

Range

1.229 - 300 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ULBP3 ELISA Kit is designed for the quantitative determination of ULBP3 in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human ULBP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and ULBP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ULBP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ULBP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested AMIGO, Aminopeptidase LRAP (ERAP2), Amnionless, Arylsulfatase A, Bcl-w, CD109, CD157, CD34, CD83, CLEC1, CLEC10A (CD301), CMG-2, CREG, Cystatin SN, Cytokeratin-8, Dectin-1 (CLEC7A), Desmocollin-3, Endoglycan, Galectin-4, HAPLN1, Jagged 1, Langerin (CD207), Lumican, Matriptase (ST14), MEP1B, Nectin-3, OX40 (TNFRSF4), OX40 Ligand (TNFSF4), p27, Pappalysin2, Plexin B3, Plexin D1, proGRP, PSA-total, Reg1B, RGM-A, ROBO2, Spinesin, TWEAK R (TNFRSF12).

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "74 - 92 %", "average": "= 81.54" }, { "sample": "Plasma", "range": "72 - 84 %", "average": "= 77.77" }, { "sample": "Cell culture media", "range": "69 - 80 %", "average": "= 77.14" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ULBP3 also known as UL16 binding protein 3 plays a significant role in immune surveillance. The protein is a member of the NKG2D ligand family and has a mass of approximately 30 kDa. ULBP3 is expressed on the cell surface of many human tissues predominantly in stressed transformed or infected cells where it becomes more prominent. The expression of ULBP3 signals to immune cells especially natural killer (NK) cells and some subsets of T-cells acting as an essential point of recognition and interaction.
Biological function summary

ULBP3 interacts with the activating receptor NKG2D to initiate immune responses against tumor cells or pathogen-infected cells. The binding of ULBP3 to NKG2D forms a part of the recognition process leading to the activation of NK cells and cytotoxic T cells enhancing their ability to destroy target cells. ULBP3 operates independently and not as part of a larger ligand complex making its interactions directly important for regulating immune function across different environments and conditions aiding in the maintenance of cellular homeostasis.

Pathways

ULBP3 participates integrally in immune signaling pathways like the NKG2D receptor-mediated pathway. Within this pathway its role in signaling enhances NK cell cytotoxicity and cytokine production. ULBP3 is related to other NKG2D ligands like MICA MICB and other ULBP molecules through this immune recognition pathway collectively contributing to immune defense mechanisms. These pathways form a critical element of the broader immune surveillance mechanism in the human body.

ULBP3 has associations with various cancers and infectious diseases. Overexpression of ULBP3 has been observed in certain types of cancers such as lymphoma where it promotes recognition by the NK cells. Another area of interest is its role in viral infections where pathogens may modulate ULBP3 expression to evade the immune system. ULBP3's interaction with proteins like NKG2D is vital in modulating immune responses during these diseases highlighting its potential both as a target for therapeutic intervention and a biomarker for disease progression.
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Product protocols

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Target data

Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.
See full target information ULBP3
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websiteProtocolBooklet
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