AB246549

Human uPAR ELISA Kit

Recombinant
SimpleStep
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(2 Publications)

Human uPAR ELISA Kit is a single-wash 90-min Simplestep used to quantify Human uPAR with a sensitivity of 21.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD87, MO3, UPAR, PLAUR, Urokinase plasminogen activator surface receptor, U-PAR, uPAR, Monocyte activation antigen Mo3

6 Images

Sandwich ELISA - Human uPAR ELISA Kit (AB246549)

sELISA

Supplier Data

Sandwich ELISA - Human uPAR ELISA Kit (AB246549)

Interpolated concentrations of native uPAR in human serum and plasma samples.

The concentrations of uPAR were measured in duplicates, interpolated from the uPAR standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean uPAR concentration was determined to be 5.34 ng/mL in serum, 4.30 ng/mL in plasma (citrate), 3.41 ng/mL in plasma (EDTA), and 4.81 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human uPAR ELISA Kit (AB246549)

Example of human uPAR standard curve in Sample Diluent NS.

The uPAR standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human uPAR ELISA Kit (AB246549)

Interpolated concentrations of native uPAR in human urine and cell culture supernatant samples.

The concentrations of uPAR were measured in duplicates, interpolated from the uPAR standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 10% and stimulated PBMC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean uPAR concentration was determined to be 12.68 ng/mL in urine and 6.32 ng/mL in stimulated PBMC supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human uPAR ELISA Kit (AB246549)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean uPAR concentration was determined to be 6.38 ng/mL with a range of 3.42 – 8.79 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human uPAR ELISA Kit (AB246549)

Human uPAR Standard Curve Comparison.

Standard Curve comparison between human uPAR SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human uPAR ELISA Kit (AB246549)

Example of human uPAR standard curve in Sample Diluent 50BS.

The uPAR standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 21.7 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human uPAR ELISA Kit (ab246549) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of uPAR protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human uPAR with 21.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.3" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 104 %", "average": "= 97" }, { "sample": "Serum", "range": "96 - 112 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "93 - 96 %", "average": "= 95" }, { "sample": "Urine", "range": "95 - 97 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "81 - 91 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "92 - 94 %", "average": "= 93" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab246549-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB246549-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246549-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246549-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB246549-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB246549-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human uPAR Capture Antibody", "number":"AB246549-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human uPAR Lyophilized Recombinant Protein", "number":"AB246549-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB246549-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB246549-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246549-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human uPAR Detector Antibody", "number":"AB246549-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Acts as a receptor for urokinase plasminogen activator (PubMed : 15677461). Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

See full target information PLAUR

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36555850

2022

The Association of Genotype and Soluble suPAR Serum Level with COVID-19-Related Lung Damage Severity.

Applications

Unspecified application

Species

Unspecified reactive species

Ludmila A Nekrasova,Anna A Shmakova,Larisa M Samokhodskaya,Karina I Kirillova,Simona S Stoyanova,Elena A Mershina,Galina B Nazarova,Kseniya A Rubina,Ekaterina V Semina,Armais A Kamalov

Cancers 14: PubMed35625969

2022

Multiphoton Microscopy Reveals DAPK1-Dependent Extracellular Matrix Remodeling in a Chorioallantoic Membrane (CAM) Model.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp Kunze,Lucas Kreiss,Vendula Novosadová,Adriana V Roehe,Sara Steinmann,Jan Prochazka,Carol I Geppert,Arndt Hartmann,Sebastian Schürmann,Oliver Friedrich,Regine Schneider-Stock

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human uPAR ELISA Kit

Sandwich ELISA - Human uPAR ELISA Kit (AB246549)

Sandwich ELISA - Human uPAR ELISA Kit (AB246549)

Sandwich ELISA - Human uPAR ELISA Kit (AB246549)

Sandwich ELISA - Human uPAR ELISA Kit (AB246549)

Sandwich ELISA - Human uPAR ELISA Kit (AB246549)

Sandwich ELISA - Human uPAR ELISA Kit (AB246549)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

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Product promise