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AB108917

Human Urokinase type Plasminogen Activator ELISA Kit

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(4 Publications)

Human Urokinase type Plasminogen Activator ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Urokinase type Plasminogen Activator in Human in Biofluids samples.

View Alternative Names

Urokinase-type plasminogen activator, U-plasminogen activator, uPA, PLAU

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum, Milk

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 45 pg/mL

Range

0.125 - 4 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Urokinase type Plasminogen Activator Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Urokinase type Plasminogen Activator (uPA) in plasma, serum, urine, milk, cell culture supernatants and tissue extracts.

A Urokinase type Plasminogen Activator specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Urokinase type Plasminogen Activator specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Urokinase type Plasminogen Activator captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108917-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108917-CMP04", "productcode":"" }, { "size":"1 x 90 µL", "name":"70X Biotinylated Human Urokinase type Plasminogen Activator Antibody", "number":"AB108917-CMP02", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108917-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108917-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Urokinase type Plasminogen Activator Microplate (12 x 8 well strips)", "number":"AB108917-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108917-CMP01", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108917-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Urokinase type Plasminogen Activator Standard", "number":"AB108917-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108917-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Urokinase also known as urokinase-type plasminogen activator (uPA) is a serine protease with a molecular mass of approximately 54 kDa. It is expressed in various tissues including the kidneys liver and blood vessels. Urokinase initially forms as pro-urokinase an inactive precursor that can convert into the active enzyme. Researchers often use assays like the direct assay to measure urokinase activity due to its role in breaking down fibrin and modulating cell adhesion and migration.
Biological function summary

Something particularly interesting about urokinase is its role in converting plasminogen to plasmin a critical enzyme in the process of fibrinolysis. Moreover it is part of a larger enzymatic complex involving the urokinase receptor (uPAR) which localizes plasminogen activation to cell surfaces. This complex mediates extracellular matrix degradation aiding in important cellular processes like tissue remodeling and wound healing.

Pathways

Urokinase plays a critical role in the fibrinolytic pathway which involves the breakdown of blood clots. It also interacts with the extracellular matrix pathway by activating plasminogen which can degrade matrix components. In these pathways urokinase closely interacts with proteins such as plasmin and its inhibitor plasminogen activator inhibitor-1 (PAI-1) which regulate the activity and localization of proteolytic events.

Urokinase is strongly linked to conditions such as deep vein thrombosis and cancer metastasis. Its overexpression or dysregulation often leads to excessive tissue degradation influencing metastasis progression. Urokinase is connected to the urokinase receptor (uPAR) in cancer facilitating cancer cell invasion and migration. Understanding its behavior in these contexts is critical for developing therapeutic strategies and targeting treatments against these diseases.
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Product protocols

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Target data

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
See full target information Urokinase-type plasminogen activator
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 14:5189-5196 PubMed29113154

2017

Synergistic inhibitory effects of an engineered antibody-like molecule ATF-Fc and trastuzumab on tumor growth and invasion in a human breast cancer xenograft mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Zhou,Hongwei Wang,Guangyuan Yu,Zhihong Wang,Xi Zheng,Haifeng Duan,Junzhong Sun

American journal of physiology. Renal physiology 309:F235-41 PubMed25972510

2015

Aberrant glomerular filtration of urokinase-type plasminogen activator in nephrotic syndrome leads to amiloride-sensitive plasminogen activation in urine.

Applications

sELISA

Species

Human

Mette Stæhr,Kristian B Buhl,René F Andersen,Per Svenningsen,Flemming Nielsen,Gitte Rye Hinrichs,Claus Bistrup,Boye L Jensen

Nephrology, dialysis, transplantation : official p 30:781-9 PubMed25609736

2015

Diabetic nephropathy is associated with increased urine excretion of proteases plasmin, prostasin and urokinase and activation of amiloride-sensitive current in collecting duct cells.

Applications

sELISA

Species

Human

Henrik Andersen,Ulla G Friis,Pernille B L Hansen,Per Svenningsen,Jan Erik Henriksen,Boye L Jensen

BMB reports 47:388-92 PubMed24286329

2013

Berberine suppresses in vitro migration of human aortic smooth muscle cells through the inhibitions of MMP-2/9, u-PA, AP-1, and NF-κB.

Applications

Unspecified application

Species

Unspecified reactive species

Su-jian Liu,Cai-xia Yin,Ming-chao Ding,Shao-you Xia,Qin-min Shen,Ji-dong Wu
View all publications
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websiteProtocolBooklet
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