Human Uromodulin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Uromodulin in Human in Serum, Plasma, Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 40 - 10000 pg/mL
- Sensitivity
- = 40 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Uromodulin. Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability (Probable). May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF (PubMed:3498215). Facilitates neutrophil migration across renal epithelia (PubMed:20798515). Uromodulin, secreted form. In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals (By similarity). Protects against urinary tract infections by binding to type 1 fimbriated E.coli (PubMed:11134021, PubMed:32616672). Binds to bacterial adhesin fimH which mediates the stable formation of bacterial aggregates, prevents the binding of E.coli to uroplakins UPK1A and UPK1B which act as urothelial receptors for type I fimbriae, and allows for pathogen clearance through micturation (PubMed:11134021, PubMed:32616672). Also promotes aggregation of other bacteria including K.pneumoniae, P.aeruginosa and S.mitis and so may also protect against other uropathogens (PubMed:32616672).
Alternative names
Uromodulin, Tamm-Horsfall urinary glycoprotein, THP, UMOD
What's included?
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Human Uromodulin ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Uromodulin in Human in Serum, Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 40 - 10000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 40 pg/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 84.71 | Range 75 - 98 % |
Sample type Plasma | Average % = 79.43 | Range 73 - 94 % |
Sample type Cell culture media | Average % = 84.18 | Range 74 - 94 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Uromodulin ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Uromodulin in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Uromodulin coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UMOD also known as uromodulin or Tamm-Horsfall protein is a glycoprotein with an approximate mass of 85 to 90 kDa. This protein is mostly expressed in the thick ascending limb of the loop of Henle in the kidney. Uromodulin is secreted into urine and represents the most abundant protein in normal urine. Its function is to contribute to the water impermeability of the thick ascending limb impact on concentration of urine and provide a barrier to bacteria adhering in the urinary tract.
- Biological function summary
Uromodulin plays a role in preventing urinary tract infections by inhibiting the adhesion of bacteria through its glycoprotein structure. It forms a gel-like layer in the urinary tract that traps pathogens. Uromodulin also participates in immune regulation serving as a modulator by interacting with immune cells. Additionally it influences renal handling of ions which impacts blood pressure regulation.
- Pathways
Uromodulin is involved in pathways associated with water and salt balance in the kidneys. This protein interacts with NKCC2 the sodium-potassium-chloride cotransporter to mediate ion transport in the thick ascending limb of the loop of Henle. Uromodulin also connects to the renin-angiotensin system helping to regulate blood pressure by influencing sodium reabsorption and vascular functions.
- Associated diseases and disorders
Uromodulin is linked to disorders such as familial juvenile hyperuricemic nephropathy and medullary cystic kidney disease type 2. These conditions stem from mutations in the UMOD gene leading to abnormal protein folding. It's noted that UMOD mutations can alter interactions with proteins like NKCC2 impacting ion transport and contributing to the pathophysiology of these kidney disorders.
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1 product image
Sandwich ELISA - Human Uromodulin ELISA Kit (ab314817)
Example of standard curve using ab314817. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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