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AB314817

Human Uromodulin ELISA Kit

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Human Uromodulin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Uromodulin in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Uromodulin, Tamm-Horsfall urinary glycoprotein, THP, UMOD

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Sandwich ELISA - Human Uromodulin ELISA Kit (AB314817)
  • sELISA

Supplier Data

Sandwich ELISA - Human Uromodulin ELISA Kit (AB314817)

Example of standard curve using ab314817. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 40 pg/mL

Range

40 - 10000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Uromodulin ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Uromodulin in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Uromodulin coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "75 - 98 %", "average": "= 84.71" }, { "sample": "Plasma", "range": "73 - 94 %", "average": "= 79.43" }, { "sample": "Cell culture media", "range": "74 - 94 %", "average": "= 84.18" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314817-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human Uromodulin Antibody-coated ELISA Plate", "number":"AB314817-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314817-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Uromodulin Protein Standard", "number":"AB314817-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Uromodulin Detection Antibody", "number":"AB314817-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314817-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314817-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314817-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB314817-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UMOD also known as uromodulin or Tamm-Horsfall protein is a glycoprotein with an approximate mass of 85 to 90 kDa. This protein is mostly expressed in the thick ascending limb of the loop of Henle in the kidney. Uromodulin is secreted into urine and represents the most abundant protein in normal urine. Its function is to contribute to the water impermeability of the thick ascending limb impact on concentration of urine and provide a barrier to bacteria adhering in the urinary tract.
Biological function summary

Uromodulin plays a role in preventing urinary tract infections by inhibiting the adhesion of bacteria through its glycoprotein structure. It forms a gel-like layer in the urinary tract that traps pathogens. Uromodulin also participates in immune regulation serving as a modulator by interacting with immune cells. Additionally it influences renal handling of ions which impacts blood pressure regulation.

Pathways

Uromodulin is involved in pathways associated with water and salt balance in the kidneys. This protein interacts with NKCC2 the sodium-potassium-chloride cotransporter to mediate ion transport in the thick ascending limb of the loop of Henle. Uromodulin also connects to the renin-angiotensin system helping to regulate blood pressure by influencing sodium reabsorption and vascular functions.

Uromodulin is linked to disorders such as familial juvenile hyperuricemic nephropathy and medullary cystic kidney disease type 2. These conditions stem from mutations in the UMOD gene leading to abnormal protein folding. It's noted that UMOD mutations can alter interactions with proteins like NKCC2 impacting ion transport and contributing to the pathophysiology of these kidney disorders.
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Product protocols

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Target data

Uromodulin. Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability (Probable). May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF (PubMed : 3498215). Facilitates neutrophil migration across renal epithelia (PubMed : 20798515).. Uromodulin, secreted form. In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals (By similarity). Protects against urinary tract infections by binding to type 1 fimbriated E.coli (PubMed : 11134021, PubMed : 32616672). Binds to bacterial adhesin fimH which mediates the stable formation of bacterial aggregates, prevents the binding of E.coli to uroplakins UPK1A and UPK1B which act as urothelial receptors for type I fimbriae, and allows for pathogen clearance through micturation (PubMed : 11134021, PubMed : 32616672). Also promotes aggregation of other bacteria including K.pneumoniae, P.aeruginosa and S.mitis and so may also protect against other uropathogens (PubMed : 32616672).
See full target information Uromodulin
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