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AB238266

Human Uteroglobin ELISA Kit

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(3 Publications)

Human Uteroglobin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Uteroglobin with a sensitivity of 1.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CC10, CCSP, UGB, SCGB1A1, Uteroglobin, Club cell phospholipid-binding protein, Club cells 10 kDa secretory protein, Secretoglobin family 1A member 1, Urinary protein 1, CCPBP, UP-1, UP1, Urine protein 1

5 Images
Sandwich ELISA - Human Uteroglobin ELISA Kit (AB238266)
  • sELISA

Supplier Data

Sandwich ELISA - Human Uteroglobin ELISA Kit (AB238266)

Interpolated concentrations of native Uteroglobin in human serum and plasma samples. The concentrations of Uteroglobin were measured in duplicates, interpolated from the Uteroglobin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 100, plasma (citrate) 1 : 100, plasma (heparin) 1 : 100, and plasma (EDTA) 1 : 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uteroglobin concentration was determined to be 13.2 ng/mL in serum, 14.5 ng/mL in plasma (citrate), 10.7 ng/ml in plasma (heparin), and 11.4 ng/ml in plasma (EDTA).

Sandwich ELISA - Human Uteroglobin ELISA Kit (AB238266)
  • sELISA

Supplier Data

Sandwich ELISA - Human Uteroglobin ELISA Kit (AB238266)

Interpolated concentrations of native Uteroglobin in female human urine, saliva, and milk samples. The concentrations of Uteroglobin were measured in duplicates, interpolated from the Uteroglobin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine (2.5%), saliva (1 : 2000), and milk (25%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uteroglobin concentration was determined to be 5.4 ng/mL in urine, 279.8 ng/mL in saliva, and 419.2 pg/mL in milk.

Sandwich ELISA - Human Uteroglobin ELISA Kit (AB238266)
  • sELISA

Supplier Data

Sandwich ELISA - Human Uteroglobin ELISA Kit (AB238266)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uteroglobin protein concentration was determined to be 14.7 ng/mL with a range of 6.0 - 30.0 ng/mL.

Sandwich ELISA - Human Uteroglobin ELISA Kit (AB238266)
  • sELISA

Supplier Data

Sandwich ELISA - Human Uteroglobin ELISA Kit (AB238266)

Human Uteroglobin Standard Curve Comparison. Standard Curve comparison between Human Uteroglobin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human Uteroglobin ELISA Kit (AB238266)
  • sELISA

Unknown

Sandwich ELISA - Human Uteroglobin ELISA Kit (AB238266)

Example of human Uteroglobin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.4 pg/mL

Range

6.3 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Uteroglobin ELISA Kit (ab238266) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Uteroglobin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Uteroglobin with 1.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "95 - 102 %", "average": "= 99" }, { "sample": "Serum", "range": "90 - 105 %", "average": "= 97" }, { "sample": "Milk", "range": "98 - 124 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "88 - 111 %", "average": "= 100" }, { "sample": "Urine", "range": "94 - 119 %", "average": "= 106" }, { "sample": "Saliva", "range": "94 - 110 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "95 - 109 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "82 - 95 %", "average": "= 89" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab238266-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB238266-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB238266-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB238266-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Uteroglobin Capture Antibody", "number":"AB238266-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Uteroglobin Lyophilized Recombinant Protein", "number":"AB238266-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB238266-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB238266-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB238266-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB238266-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human Uteroglobin Detector Antibody (Lyophilized)", "number":"AB238266-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Uteroglobin also known as Clara cell secretory protein (CC16) is a small secreted protein with a molecular mass of approximately 16 kDa. Expressed chiefly in the respiratory epithelium of the lungs particularly by Clara cells it is also present in other tissues such as the kidney and uterus. Uteroglobin acts as an anti-inflammatory and immunomodulatory protein playing an important role in protecting epithelial cells from damage by various harmful agents.
Biological function summary

Uteroglobin serves as a multifunctional secretory agent contributing to the regulation of inflammation and tissue remodeling. It does not form part of a larger complex but acts independently. By binding and inhibiting pro-inflammatory mediators like phospholipase A2 Uteroglobin reduces the production of arachidonic acid derivatives which are involved in inflammatory processes. Additionally it acts as a chemoattractant for certain immune cells further modulating immune responses.

Pathways

Uteroglobin integrates into the immune response and inflammation pathways. It indirectly interacts with proteins such as cytokines and chemokines influencing pathways like the arachidonic acid metabolism pathway and the leukocyte extravasation signaling pathway. These interactions reduce inflammatory response and regulate immune cell movement demonstrating its anti-inflammatory function.

Low levels of Uteroglobin have been associated with respiratory conditions such as asthma and chronic obstructive pulmonary disease (COPD). By affecting inflammation pathways Uteroglobin interacts with inflammatory cytokines which exacerbate these conditions. Additionally its connection to proteins involved in these pathways makes it a potential biomarker for assessing lung injury and monitoring disease progression.
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Product protocols

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Target data

Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
See full target information SCGB1A1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of advanced research 59:161-171 PubMed37330047

2023

Effects of lifestyle and associated diseases on serum CC16 suggest complex interactions among metabolism, heart and lungs.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie Rohmann,Paula Stürmer,Corinna Geisler,Kristina Schlicht,Carina Knappe,Katharina Hartmann,Kathrin Türk,Tim Hollstein,Alexia Beckmann,Anna K Seoudy,Ulla Becker,Perdita Wietzke-Braun,Ute Settgast,Florian Tran,Philip Rosenstiel,Jan H Beckmann,Witigo von Schönfels,Stephan Seifert,Jan Heyckendorf,Andre Franke,Stefan Schreiber,Dominik M Schulte,Matthias Laudes

Scientific reports 13:4749 PubMed36959289

2023

Pneumoproteins and biomarkers of inflammation and coagulation do not predict rapid lung function decline in people living with HIV.

Applications

Unspecified application

Species

Unspecified reactive species

David M MacDonald,Sarah Samorodnitsky,Chris H Wendt,Jason V Baker,Gary Collins,Monica Kruk,Eric F Lock,Roger Paredes,Selvamuthu Poongulali,Danielle O Weise,Alan Winston,Robin Wood,Ken M Kunisaki

Frontiers in immunology 13:1037115 PubMed36311771

2022

Brief Research Report: Serum clara cell 16 kDa protein levels are increased in patients hospitalized for severe SARS-CoV-2 or sepsis infection.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie Rohmann,Paula Stürmer,Corinna Geisler,Kristina Schlicht,Katharina Hartmann,Kathrin Türk,Tim Hollstein,Florian Tran,Philip Rosenstiel,Andre Franke,Jan Heyckendorf,Stefan Schreiber,Dominik M Schulte,Matthias Laudes
View all publications
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Product promise

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