Human VAP-1 ELISA Kit
- Recombinant
- SimpleStep
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Human VAP-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human VAP-1 with a sensitivity of 8.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
VAP1, AOC3, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, Copper amine oxidase, HPAO, Semicarbazide-sensitive amine oxidase, Vascular adhesion protein 1, SSAO, VAP-1
- sELISA
Supplier Data
Sandwich ELISA - Human VAP-1 ELISA Kit (AB243684)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VAP-1 concentration where detectable was determined to be 512.9 ng/mL with a range of 0 – 855.8 ng/mL when diluted to 0.1% serum.
- sELISA
Supplier Data
Sandwich ELISA - Human VAP-1 ELISA Kit (AB243684)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VAP-1 concentration was determined to be 345.5 ng/mL with a range of 183.1 – 454.8 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human VAP-1 ELISA Kit (AB243684)
Example of human VAP-1 standard curve in Sample Diluent NS.
The VAP-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human VAP-1 ELISA Kit (AB243684)
Interpolated concentrations of spike VAP-1 in cell culture media samples.
The concentrations of VAP-1 were measured in duplicates, interpolated from the VAP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Human VAP-1 ELISA Kit (AB243684)
Human VAP-1 Standard Curve Comparison.
Standard Curve comparison between human VAP-1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Human VAP-1 ELISA Kit (AB243684)
Interpolated concentrations of native VAP-1 in human serum and plasma samples.
The concentrations of VAP-1 were measured in duplicates, interpolated from the VAP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 400, plasma (citrate) 1 : 200, plasma (EDTA) 1 : 200 and plasma (heparin) 1 : 400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VAP-1 concentration was determined to be 238.1 ng/mL in serum, 183.6 ng/mL in plasma (citrate), 196.6 ng/mL in plasma (EDTA) and 279.4 ng/mL in plasma (heparin).
Reactivity data
Product details
Human VAP-1 ELISA Kit (ab243684) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VAP-1 protein in cit plasma, edta plasma, hep plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human VAP-1 with 8.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
VAP1 plays important roles in leukocyte trafficking what makes it important for immune response and inflammation. It facilitates the adhesion and transmigration of leukocytes across the endothelium into tissues. VAP1 is part of numerous adhesion molecule networks that help regulate the immune response. Its ability to interact with diverse substrates also affects vascular function and integrity.
Pathways
VAP1 interacts with immune responses and inflammation-related pathways. It influences the leukocyte adhesion cascade which integrates with the immune cell signaling pathways. Proteins like Integrins and Selectins relate to VAP1 in these processes as they are also involved in mediating cell adhesion to the endothelial cells thereby orchestrating cell movement and positioning essential for immune defense.
Product protocols
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Target data
Complementary Products
Product promise
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