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Human Vasopressin-neurophysin 2-copeptin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Vasopressin-neurophysin 2-copeptin in Tissue Extracts samples.

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Images

Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (AB289701), expandable thumbnail
  • Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (AB289701), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

40.625 - 2600 pg/mL

Assay time

1h 30m

Sensitivity

= 12.034 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Neurophysin 2Specifically binds vasopressin.Arg-vasopressinHas a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels. Acts by binding to vasopressin receptors (V1bR/AVPR1B, V1aR/AVPR1A, and V2R/AVPR2) (PubMed:18174156).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human Vasopressin-neurophysin 2-copeptin Capture Antibody
1 x 600 µL
10X Human Vasopressin-neurophysin 2-copeptin Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent 4BR
1 x 6 mL
Human Vasopressin-neurophysin 2-copeptin Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human Vasopressin-neurophysin 2-copeptin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Vasopressin-neurophysin 2-copeptin in Tissue Extracts samples.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

40.625 - 2600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 12.034 pg/mL

Precision

Intra assay

Sample

Extract

n

8

mean

-

SD

-

C.V.

5.2

Inter assay

Sample

Extract

n

3

mean

-

SD

-

C.V.

11.3

Recovery

Sample specific recovery

Sample type

Tissue Extracts

Average %

= 95

Range

94 - 96 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Vasopressin-neurophysin 2-copeptin ELISA Kit (ab289701) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Vasopressin-neurophysin 2-copeptin in tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vasopressin-neurophysin 2-copeptin with 12.034 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (ab289701), expandable thumbnail

    Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (ab289701)

    Interpolated concentrations of human Vasopressin Neurophysin 2-copeptin in human brain stem extract.

    Interpolated concentration of native Vasopressin Neurophysin 2-copeptin was measured in duplicate at different sample concentrations. Undiluted samples are 600 μg/mL human brain stem extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

  • Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (ab289701), expandable thumbnail

    Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (ab289701)

    Example of human Vasopressin Neurophysin 2-copeptin standard curve in 1X Cell Extraction Buffer PTR.

    Example of human Vasopressin Neurophysin 2-copeptin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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