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AB289701

Human Vasopressin-neurophysin 2-copeptin ELISA Kit

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(1 Publication)

Human Vasopressin-neurophysin 2-copeptin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Vasopressin-neurophysin 2-copeptin with a sensitivity of 12.034 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ARVP, VP, AVP, Vasopressin-neurophysin 2-copeptin, AVP-NPII

2 Images
Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (AB289701)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (AB289701)

Example of human Vasopressin Neurophysin 2-copeptin standard curve in 1X Cell Extraction Buffer PTR.

Example of human Vasopressin Neurophysin 2-copeptin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (AB289701)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (AB289701)

Interpolated concentrations of human Vasopressin Neurophysin 2-copeptin in human brain stem extract.

Interpolated concentration of native Vasopressin Neurophysin 2-copeptin was measured in duplicate at different sample concentrations. Undiluted samples are 600 μg/mL human brain stem extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 12.034 pg/mL

Range

40.625 - 2600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Vasopressin-neurophysin 2-copeptin ELISA Kit (ab289701) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Vasopressin-neurophysin 2-copeptin in tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vasopressin-neurophysin 2-copeptin with 12.034 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.3" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "94 - 96 %", "average": "= 95" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289701-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289701-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289701-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289701-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289701-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289701-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB289701-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Vasopressin-neurophysin 2-copeptin Capture Antibody", "number":"AB289701-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB289701-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Vasopressin-neurophysin 2-copeptin Lyophilized Recombinant Protein", "number":"AB289701-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB289701-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Vasopressin-neurophysin 2-copeptin Detector Antibody", "number":"AB289701-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Arg-vasopressin. Has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels. Acts by binding to vasopressin receptors (V1bR/AVPR1B, V1aR/AVPR1A, and V2R/AVPR2).. Neurophysin 2. Specifically binds vasopressin.
See full target information AVP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 14:8002-8008 PubMed36505320

2022

The value of Copeptin, myocardial fatty acid-binding protein and myocardial markers in the early diagnosis of acute myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Guan,Tian Liang,Jianyun Fan,Wenyou Yang,Dongliang Wu,Xinguo Li
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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