Human Vasopressin-neurophysin 2-copeptin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Vasopressin-neurophysin 2-copeptin in Tissue Extracts samples.
Colorimetric
Tissue Extracts
Sandwich (quantitative)
Human
40.625 - 2600 pg/mL
1h 30m
= 12.034 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Neurophysin 2Specifically binds vasopressin.Arg-vasopressinHas a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels. Acts by binding to vasopressin receptors (V1bR/AVPR1B, V1aR/AVPR1A, and V2R/AVPR2) (PubMed:18174156).
ARVP, VP, AVP, Vasopressin-neurophysin 2-copeptin, AVP-NPII
Human Vasopressin-neurophysin 2-copeptin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Vasopressin-neurophysin 2-copeptin in Tissue Extracts samples.
Colorimetric
Tissue Extracts
Sandwich (quantitative)
Human
40.625 - 2600 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 12.034 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 5.2 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 11.3 |
Sample type | Average % | Range |
---|---|---|
Sample type Tissue Extracts | Average % = 95 | Range 94 - 96 % |
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Human Vasopressin-neurophysin 2-copeptin ELISA Kit (ab289701) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Vasopressin-neurophysin 2-copeptin in tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vasopressin-neurophysin 2-copeptin with 12.034 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Interpolated concentrations of human Vasopressin Neurophysin 2-copeptin in human brain stem extract.
Interpolated concentration of native Vasopressin Neurophysin 2-copeptin was measured in duplicate at different sample concentrations. Undiluted samples are 600 μg/mL human brain stem extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Example of human Vasopressin Neurophysin 2-copeptin standard curve in 1X Cell Extraction Buffer PTR.
Example of human Vasopressin Neurophysin 2-copeptin standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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