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AB47355

Human VCAM-1 ELISA Set (without plates)

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(5 Publications)

Human VCAM-1 ELISA Set (without plates) is a Sandwich ELISA for the measurement of Human VCAM-1 ELISA Set (without plates) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD106, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1

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Sandwich ELISA - Human VCAM-1 ELISA Set (without plates) (AB47355)
  • sELISA

Supplier Data

Sandwich ELISA - Human VCAM-1 ELISA Set (without plates) (AB47355)

VCAM-1 Standard curve ranging from 1.56 to 50 ng/mL

Key facts

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 0.6 ng/mL

Range

1.56 - 50 ng/mL

Assay time

1h 45m

Assay Platform

Reagents

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Reactivity data

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Product details

Abcam's VCAM-1 Human in vitro ELISA Set is designed for the quantitative measurement of VCAM-1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

This ELISA Set is intended to measure human VCAM-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

The VCAM-1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

Recognizes both natural and recombinant human VCAM1

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.
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Product protocols

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Target data

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed : 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed : 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed : 22970700). Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed : 35210567).
See full target information VCAM1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Toxicon: X 26:100226 PubMed40525140

2025

Pathophysiological role of endothelial biomarkers in sp. venom-induced renal dysfunction and the therapeutic effect of antivenom.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole Coelho Lopes,Gdayllon Cavalcante Meneses,Ranieri Sales de Souza Santos,Leticia Machado de Araújo,Bruna Viana Barroso Martins,Katarina Maria Dos Reis Araújo,Valéria Holanda Nogueira de Aquino,Igor Moreira de Almeida,Sandra Mara Brasileiro Mota,Geraldo Bezerra da Silva Junior,Camila Eleuterio Rodrigues,Elizabeth De Francesco Daher,Polianna Lemos Moura Moreira Albuquerque,Alice Maria Costa Martins

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 56:e12728 PubMed37585916

2023

Predictors of mortality in critically ill patients with COVID-19 and diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

A P P Lázaro,M S Zaranza,G C Meneses,N L Aragão,M V P Freire,Á R Guimarães,A M Beliero,M M P Dantas,L C Forte,A M C Martins,E F Daher,P L M M Albuquerque,G B da Silva Júnior

Tropical medicine & international health : TM & IH 27:727-734 PubMed35761507

2022

Endothelial biomarkers as predictors for haemodialysis need in severe leptospirosis patients (Weil's disease).

Applications

Unspecified application

Species

Unspecified reactive species

Gdayllon Cavalcante Meneses,Pedro Eduardo Andrade de Carvalho Gomes,Gabriela Studart Galdino,Gabriela Freire Bezerra,Ranieri Sales de Souza Santos,Alice Maria Costa Martins,Geraldo Bezerra da Silva Junior,Alexandre Braga Libório,Roberto da Justa Pires Neto,Elizabeth De Francesco Daher

Revista do Instituto de Medicina Tropical de Sao Paulo 63:e44 PubMed34161550

2021

Endothelial activation is associated with albuminuria in multibacillary leprosy.

Applications

Unspecified application

Species

Unspecified reactive species

Louise Donadello Tessarolo,Gdayllon Cavalcante Meneses,Gabriela Freire Bezerra,Thaiany Pereira da Rocha,Isabella Evelyn Prado de Azevedo,Geraldo Bezerra Silva Junior,Maria Araci Andrade Pontes,Elizabeth De Francesco Daher,Alice Maria Costa Martins

Brazilian journal of medical and biological resear 52:e8658 PubMed31778438

2019

Interleukin-6 and adhesion molecules VCAM-1 and ICAM-1 as biomarkers of post-acute myocardial infarction heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

D O C Lino,I A Freitas,G C Meneses,A M C Martins,E F Daher,J H C Rocha,G B Silva Junior
View all publications
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