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AB100661

Human VCAM1 ELISA Kit

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(4 Publications)

Human VCAM1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human VCAM1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD106, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1

2 Images
Sandwich ELISA - Human VCAM1 ELISA Kit (AB100661)
  • sELISA

Unknown

Sandwich ELISA - Human VCAM1 ELISA Kit (AB100661)

Representative standard curve using ab100661

Sandwich ELISA - Human VCAM1 ELISA Kit (AB100661)
  • sELISA

Unknown

Sandwich ELISA - Human VCAM1 ELISA Kit (AB100661)

Representative standard curve using ab100661

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 0.3 ng/mL

Range

0.247 - 60 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's VCAM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VCAM1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human VCAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and VCAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VCAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VCAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 104 %", "average": "= 98.76" }, { "sample": "Serum", "range": "83 - 103 %", "average": "= 94.42" }, { "sample": "Plasma", "range": "82 - 102 %", "average": "= 93.58" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100661-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100661-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100661-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human VCAM1", "number":"AB100661-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100661-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100661-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100661-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"320X HRP-Streptavidin Concentrate", "number":"AB100661-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human VCAM1 Standard (lyophilized)", "number":"AB100661-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"VCAM1 Microplate (12 x 8 wells)", "number":"AB100661-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.
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Product protocols

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Target data

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed : 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed : 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed : 22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed : 35210567).
See full target information VCAM1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:1090877 PubMed36591243

2022

Piperlongumine alleviates corneal allograft rejection suppressing angiogenesis and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Fan,Jini Qiu,Tianjie Yuan,Jing Zhang,Jianjiang Xu

The Journal of international medical research 49:3000605211012569 PubMed34024182

2021

Identification of adipose tissue-related predictors of the reduction in cardiovascular risk induced by metabolic surgery.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Melchor-López,Juan Antonio Suárez-Cuenca,Diana Zaineff Banderas-Lares,Gustavo De la Peña-Sosa,Moisés Salamanca-García,Eduardo Vera-Gómez,Alejandro Hernández-Patricio,Juan Ariel Gutiérrez-Buendía,Carlos Ramiro Zamora-Alemán,Sofía Lizeth Alcaráz-Estrada,Moisés Ortiz-Fernández,Jesús Montoya-Ramírez,Omar Felipe Gaytán-Fuentes,Rebeca Pérez-Cabeza de Vaca,Mónica Escamilla-Tilch,Juan Antonio Pineda-Juárez,Mario Antonio Téllez-González,Paul Mondragón-Terán,Martha Eunice Rodríguez-Arellano,Alejandra Contreras-Ramos,Silvia García,Rolando Efraín Hernández-Muñoz

Molecular medicine reports 16:2588-2594 PubMed28713935

2017

Curcumin pretreatment protects against PM2.5‑induced oxidized low‑density lipoprotein‑mediated oxidative stress and inflammation in human microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Shi,Huiping Deng,Min Zhang

Nephrology, dialysis, transplantation : official p 29:926-33 PubMed24452849

2014

Short-term efficacy and safety of sitagliptin treatment in long-term stable renal recipients with new-onset diabetes after transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Thea Anine Strøm Halden,Anders Åsberg,Karen Vik,Anders Hartmann,Trond Jenssen
View all publications
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