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AB223591

Human VCAM1 ELISA Kit

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(8 Publications)

Human VCAM1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human VCAM1 with a sensitivity of 37 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

CD106, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1

6 Images
ELISA - Human VCAM1 ELISA Kit (AB223591)
  • ELISA

Supplier Data

ELISA - Human VCAM1 ELISA Kit (AB223591)
Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)
  • sELISA

Supplier Data

Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)

Example of human VCAM1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)
  • sELISA

Supplier Data

Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)

Interpolated concentrations of native VCAM1 in human serum and plasma, and interpolated concentrations of spiked VCAM1 in cell culture supernatant samples.

The concentrations of VCAM1 were measured in duplicates, interpolated from the VCAM1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma (citrate) 2.5%, plasma (EDTA) 2.5%, plasma (heparin) 2.5%, and RPMI media 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VCAM1 concentration was determined to be 1196 ng/mL in neat serum, 1092 ng/mL in neat plasma (citrate), 1275 ng/mL in neat plasma (EDTA), and 976 ng/mL in neat plasma (heparin).

Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)
  • sELISA

Supplier Data

Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)

Serum from eight individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VCAM1 concentration was determined to be 1007 ng/mL with a range of 690 – 1501 ng/mL.

Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)
  • sELISA

Unknown

Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)

Human VCAM1 Competitor standard curve comparison.

Standard curve comparison between Human VCAM1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)
  • sELISA

Supplier Data

Sandwich ELISA - Human VCAM1 ELISA Kit (AB223591)

Representative image of Human VCAM1 ELISA Kit (ab223591)

Components shown from left to right :

- Sample diluent NS

- 10X Human VCAM1 Detector Antibody

- Human VCAM1 Lyophilised Purified Protein (2 vials)

- Human VCAM1 Capture Antibody (Lyophilised)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 37 pg/mL

Range

0.14 - 9 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human VCAM1 ELISA Kit ab223591 is a rapid single-wash 90-min Sandwich ELISA to measure Human VCAM1 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 37 pg/mL.

How the assay works

Human VCAM1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human VCAM1 ELISA Kit ab223591 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-VCAM1 antibody [EPR20348-122] - BSA and Azide free (Capture) ab244923
- Anti-VCAM1 antibody [EPR20347-57] - BSA and Azide free (Detector) ab244923
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.23" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" } ]
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Recovery

[ { "sample": "Serum", "range": "88 - 104 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "88 - 109 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "89 - 103 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "92 - 102 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "92 - 108 %", "average": "= 101" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab223591-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB223591-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB223591-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB223591-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB223591-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB223591-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human VCAM1 Capture Antibody (Lyophilized)", "number":"AB223591-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human VCAM1 Lyophilized Recombinant Protein", "number":"AB223591-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB223591-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human VCAM1 Detector Antibody", "number":"AB223591-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB223591-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.
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Product protocols

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Target data

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed : 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed : 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed : 22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed : 35210567).
See full target information VCAM1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:11535 PubMed38773159

2024

Gamma radiation assisted green synthesis of hesperidin-reduced graphene oxide nanocomposite targeted JNK/SMAD4/MMP2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmad S Kodous,Eman O Taha,Dina F El-Maghraby,Asmaa A Hassana,M M Atta

Heliyon 9:e17366 PubMed37426804

2023

circSnd1 promotes atherosclerosis progression through the miR-485-3p/Olr1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Yang,Yuhao Lin,Chao Wang,Pengcheng Fan

Journal of applied toxicology : JAT 43:1613-1629 PubMed37278136

2023

Integrative proteomics and metabolomics analysis of non-observable acute effect level PM induced accumulative effects in AC16 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bingru Nan,Xia Sun,Shijing Yang,Qingyu Huang,Heqing Shen

Redox biology 62:102681 PubMed37003179

2023

MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nunzia D'Onofrio,Francesco Prattichizzo,Elisa Martino,Camilla Anastasio,Luigi Mele,Rosalba La Grotta,Celestino Sardu,Antonio Ceriello,Raffaele Marfella,Giuseppe Paolisso,Maria Luisa Balestrieri

Journal of clinical laboratory analysis 36:e24685 PubMed36045604

2022

Serum VCAM-1 and ICAM-1 measurement assists for MACE risk estimation in ST-segment elevation myocardial infarction patients.

Applications

Unspecified application

Species

Unspecified reactive species

Jiancai Yu,Yongxing Liu,Wanzhong Peng,Zesheng Xu

Theranostics 12:4564-4580 PubMed35832090

2022

FGFR blockade boosts T cell infiltration into triple-negative breast cancer by regulating cancer-associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yushen Wu,Ziying Yi,Jie Li,Yuxian Wei,Rui Feng,Jiazhou Liu,Jiefeng Huang,Yuru Chen,Xiaoyu Wang,Jiazheng Sun,Xuedong Yin,Yunhai Li,Jingyuan Wan,Li Zhang,Jing Huang,Huimin Du,Xiaoyi Wang,Qin Li,Guosheng Ren,Hongzhong Li

The Journal of international medical research 49:300060520985365 PubMed33845640

2021

Effect of a three-piece inflatable penile prosthesis combined with a phosphodiesterase-5 inhibitor on erectile dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wang,Peng Wu,Qiang Liu,Liangliang Ben,Geng Chen,Zhijuan Han,Hui Peng

Journal of cell communication and signaling 15:237-250 PubMed33058043

2020

Long non-coding RNA DIO3OS/let-7d/NF-κB2 axis regulates cells proliferation and metastasis of thyroid cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mingming Wang,Jin Li,Zhongkun Zuo,Chutong Ren,Tenglong Tang,Chen Long,Yi Gong,Fei Ye,Zhihong Wang,Jiangsheng Huang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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