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Human VCAM1 ELISA Kit (CD106) is a Sandwich (quantitative) ELISA kit for the measurement of Human VCAM1 (CD106) in Human in Plasma, Cell culture supernatant, Serum samples.
Alternative names=Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
1.56 - 50 ng/mL
- Assay time
1h 45m
- Sensitivity
< 0.6 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Human VCAM1 ELISA Kit (CD106) is a Sandwich (quantitative) ELISA kit for the measurement of Human VCAM1 (CD106) in Human in Plasma, Cell culture supernatant, Serum samples.
Alternative names=Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
1.56 - 50 ng/mL
- Assay time
1h 45m
- Assay Platform
Microplate
- Sensitivity
< 0.6 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample A | n 8 | mean 48.79 ng/mL | SD 0.221 | C.V. 0.45 |
Sample Sample B | n 8 | mean 5.81 ng/mL | SD 0.132 | C.V. 2.27 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample A | n 28 | mean 49.69 ng/mL | SD 0.72 | C.V. 1.44 |
Sample Sample B | n 28 | mean 5.95 ng/mL | SD 0.354 | C.V. 5.94 |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Abcam's Human VCAM1 (CD106) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VCAM1 in cell culture supernatants, buffered solutions, serum, plasma, and other body fluids.
A monoclonal antibody specific for VCAM1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known VCAM1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for VCAM1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of VCAM1 present in the samples.
This kit will recognize both endogenous and recombinant Human VCAM1.
Get results in 90 minutes with Human VCAM1 ELISA Kit (CD106) (ab187393) from our SimpleStep ELISA® range.
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Product protocols
- Visit the general protocols
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