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Human VCAM1 ELISA Kit (CD106) is a Sandwich (quantitative) ELISA kit for the measurement of Human VCAM1 (CD106) in Human in Plasma, Cell culture supernatant, Serum samples.

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

1.56 - 50 ng/mL

Assay time

1h 45m

Sensitivity

< 0.6 ng/mL

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Shipping To:
United States

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

What's included?

2 x 96 Tests
Components
10X Standard Diluent Buffer
1 x 15 mL
200X Wash Buffer
2 x 10 mL
Biotinylated Antibody Diluent
1 x 13 mL
Biotinylated anti-VCAM1
2 x 400 µL
Chromogen TMB Substrate Solution
1 x 24 mL
Control
4 x 1 Vial
HRP Diluent
1 x 12 mL
Stop Reagent
2 x 11 mL
Streptavidin-HRP
4 x 5 µL
VCAM1 Microplate (12 x 8 well strips)
2 x 1 Unit
VCAM1 Standard (lyophilized)
4 x 1 Vial

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Human VCAM1 ELISA Kit (CD106) is a Sandwich (quantitative) ELISA kit for the measurement of Human VCAM1 (CD106) in Human in Plasma, Cell culture supernatant, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

1.56 - 50 ng/mL

Assay time

1h 45m

Assay Platform

Microplate

Sensitivity

< 0.6 ng/mL

Precision

Intra assay

Sample
Sample A
n
8
mean
48.79 ng/mL
SD
0.221
C.V.
0.45
Sample
Sample B
n
8
mean
5.81 ng/mL
SD
0.132
C.V.
2.27

Inter assay

Sample
Sample A
n
28
mean
49.69 ng/mL
SD
0.72
C.V.
1.44
Sample
Sample B
n
28
mean
5.95 ng/mL
SD
0.354
C.V.
5.94

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Abcam's Human VCAM1 (CD106) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VCAM1 in cell culture supernatants, buffered solutions, serum, plasma, and other body fluids.

A monoclonal antibody specific for VCAM1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known VCAM1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for VCAM1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of VCAM1 present in the samples.

This kit will recognize both endogenous and recombinant Human VCAM1.

Get results in 90 minutes with Human VCAM1 ELISA Kit (CD106) (ab187393) from our SimpleStep ELISA® range.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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