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AB46118

Human VCAM1 ELISA Kit (CD106)

5

(1 Review)

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(4 Publications)

Human VCAM1 ELISA Kit (CD106) is a Sandwich (quantitative) ELISA for the measurement of Human VCAM1 (CD106) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD106, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1

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Sandwich ELISA - Human VCAM1 ELISA Kit (CD106) (AB46118)
  • sELISA

Supplier Data

Sandwich ELISA - Human VCAM1 ELISA Kit (CD106) (AB46118)

Representative Standard Curve using ab46118.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 0.6 ng/mL

Range

1.56 - 50 ng/mL

Assay time

1h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human VCAM1 (CD106) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VCAM1 in cell culture supernatants, buffered solutions, serum, plasma, and other body fluids.

A monoclonal antibody specific for VCAM1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known VCAM1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for VCAM1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of VCAM1 present in the samples.

This kit will recognize both endogenous and recombinant Human VCAM1.

Get results in 90 minutes with Human VCAM1 ELISA Kit (CD106) (ab187393) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 28, "mean": "49.69 ng/mL", "standardDeviation": "0.72", "coefficientOfVariability": "1.44" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 28, "mean": "5.95 ng/mL", "standardDeviation": "0.354", "coefficientOfVariability": "5.94" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "48.79 ng/mL", "standardDeviation": "0.221", "coefficientOfVariability": "0.45" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "5.81 ng/mL", "standardDeviation": "0.132", "coefficientOfVariability": "2.27" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46118-2x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46118-CMP03" }, { "size":"4 x 1 Vial", "name":"Control", "number":"AB46118-CMP06" }, { "size":"4 x 1 Vial", "name":"VCAM1 Standard (lyophilized)", "number":"AB46118-CMP08" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46118-CMP02" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-VCAM1", "number":"AB46118-CMP04" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46118-CMP11" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46118-CMP09" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46118-CMP05" }, { "size":"2 x 1 Unit", "name":"VCAM1 Microplate (12 x 8 well strips)", "number":"AB46118-CMP07" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46118-CMP01" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46118-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46118-1x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46118-CMP03", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46118-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"VCAM1 Microplate (12 x 8 well strips)", "number":"AB46118-CMP07", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46118-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Control", "number":"AB46118-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"VCAM1 Standard (lyophilized)", "number":"AB46118-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46118-CMP10", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-VCAM1", "number":"AB46118-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46118-CMP09", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46118-CMP11", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46118-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.
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Product protocols

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Target data

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed : 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed : 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed : 22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed : 35210567).
See full target information VCAM1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Heart international 16:75-84 PubMed36741104

2022

Effect of Endothelial Adhesion Molecules on Atrial Fibrillation: A Systematic Review and Meta-analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Mehran Rahimi,Leili Faridi,Leila Nikniaz,Sara Daneshvar,Amirreza Naseri,Mohammadreza Taban-Sadeghi,Hesam Manaflouyan,Javad Shahabi,Nizal Sarrafzadegan

Journal of infection in developing countries 15:289-296 PubMed33690213

2021

Cardiovascular risks in Asian HIV-infected patients receiving boosted-protease inhibitor-based antiretroviral treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Sakaewan Ounjaijean,Kanokwan Kulprachakarn,Linda Aurpibul,Quanhathai Kaewpoowat,Kongsak Boonyapranai,Romanee Chaiwarith,Supapong Arwon,Khuanchai Supparatpinyo,Kittipan Rerkasem

Open life sciences 14:638-646 PubMed33817202

2019

A Allele of Rs5498 and Rs3181092 is Correlated with Increased Risk for Periodontal Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qijun Sun,Zongxin Zhang,Yuejian Ou

Journal of cardiothoracic surgery 12:70 PubMed28821262

2017

Pre-operative serum VCAM-1 as a biomarker of atrial fibrillation after coronary artery bypass grafting.

Applications

Unspecified application

Species

Unspecified reactive species

Leanne Harling,Jonathan Lambert,Hutan Ashrafian,Ara Darzi,Nigel J Gooderham,Thanos Athanasiou
View all publications
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