AB210968

Human VE Cadherin ELISA Kit

Recombinant
SimpleStep
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(5 Publications)

Human VE Cadherin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human VE Cadherin with a sensitivity of 331 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD144, Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CDH5

4 Images

Sandwich ELISA - Human VE Cadherin ELISA Kit (AB210968)

sELISA

Supplier Data

Sandwich ELISA - Human VE Cadherin ELISA Kit (AB210968)

Interpolated concentrations of native VE-Cadherin in human plasma samples.

The concentrations of VE-Cadherin were measured in duplicates, interpolated from the VE-Cadherin standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (citrate) 1 : 100 and plasma (EDTA) 1 : 67. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VE-Cadherin concentration was determined to be 1091 ng/mL in plasma (citrate) and 809 ng/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human VE Cadherin ELISA Kit (AB210968)

Interpolated concentrations of native VE-Cadherin in human serum, and plasma samples.

The concentrations of VE-Cadherin were measured in duplicates, interpolated from the VE-Cadherin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 100 and plasma (heparin) 1 : 67. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VE-Cadherin concentration was determined to be 919 ng/mL in serum and 1,400 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human VE Cadherin ELISA Kit (AB210968)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VE-Cadherin concentration was determined to be 908 ng/mL with a range of 628 – 1,527 ng/mL

sELISA

Supplier Data

Sandwich ELISA - Human VE Cadherin ELISA Kit (AB210968)

Example of Human VE-Cadherin standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 331 pg/mL

Range

0.63 - 40 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human VE Cadherin ELISA Kit (ab210968) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VE Cadherin protein in cell culture supernatant, plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human VE Cadherin with 331 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

VE-Cadherin (also known as Cadherin 5 or CD144) is a calcium dependent cell-cell adhesion glycoprotein. VE-Cadherin is a member of the cadherin superfamily, which interact with themselves in connecting cells. VE-Cadherin is expressed in endothelial cells and has role establishing and maintaining endothelial polarity and intercellular junctions.

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Precision

[ { "reproducibilityType": "Inter", "sample": "EDTA Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "EDTA Plasma", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "112 - 115 %", "average": "= 119" }, { "sample": "Serum", "range": "91 - 103 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "84 - 99 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "96 - 101 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "110 - 121 %", "average": "= 116" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VE Cadherin also known as CD144 or Cadherin-5 is a protein of about 135 kDa involved in cell adhesion. It is a transmembrane protein characterized by its presence at endothelial cell junctions. VE Cadherin belongs to the cadherin superfamily and its expression predominantly occurs in vascular endothelial cells. This protein facilitates the adhesion between adjacent endothelial cells maintaining the integrity of endothelial barriers.

Biological function summary

VE Cadherin performs essential roles in vascular development and permeability. It is a component of adherens junctions and connects with catenins to mediate cell-cell adhesion through calcium-dependent binding. This protein's adhesive function is critical for blood vessel maturation and response to inflammatory stimuli. Altering VE Cadherin expression impacts vascular stability and endothelial cell layering.

Pathways

VE Cadherin interacts with the Wnt and VEGF signaling pathways. It plays a role in the regulation of endothelial cell survival and migration. The interaction with β-catenin is important in the Wnt pathway influencing the transcription of target genes that modulate cell behavior. In the VEGF pathway VE Cadherin's association with protein kinase C highlights its involvement in endothelial permeability and angiogenesis processes.

VE Cadherin dysregulation links to cancer progression and atherosclerosis. Its role in maintaining endothelial barrier integrity implicates it in tumor metastasis where disrupted cell adhesion facilitates cancer cell dissemination. Additionally altered VE Cadherin expression or function associates with atherosclerotic disease where damaged vascular endothelium leads to plaque formation. The connection of VE Cadherin with β-catenin in these conditions highlights its significance in pathological angiogenesis and vascular remodeling.

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Product protocols

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Target data

Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (PubMed : 21269602). This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (By similarity). It associates with alpha-catenin forming a link to the cytoskeleton (PubMed : 10861224). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (By similarity). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (By similarity). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (PubMed : 20332120). Positively regulates reorientation of actin stress fibers and endothelial cell reorientation in response to cellular mechantransduction (PubMed : 25795300). Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (PubMed : 20332120). Associates with CTNND1/p120-catenin to control CADH5 endocytosis (By similarity).

See full target information CDH5

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

RSC advances 15:12609-12621 PubMed40264865

2025

Functionalized screen-printed electrodes for non-invasive detection of vascular-endothelial cadherin in extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

William Meza-Morales,Sahimy Ayus-Martinez,Jesus Jimenez-Osorio,Maria Buendia-Otero,Luis López,David Suleiman,Edu Suarez,Donald O Freytes,Lisandro Cunci,Camilo Mora

Biomedicines 11: PubMed36672669

2023

Differential Serum Proteomic Signatures between Acute Aortic Dissection and Acute Myocardial Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

You-Cian Lin,Jeen-Chen Chen,Jiunn-Min Lin,Chih-Hsiang Hsu,Ching-Feng Wu,Shao-Hsuan Kao

Frontiers in neurology 12:721118 PubMed34531816

2021

MMP-9 as a Biomarker for Predicting Hemorrhagic Strokes in Moyamoya Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Junlin Lu,Jingyi Wang,Zhidong Lin,Guangchao Shi,Rong Wang,Yahui Zhao,Yuanli Zhao,Jizong Zhao

Journal of cancer research and clinical oncology 145:411-427 PubMed30483898

2018

Effects of VEGFR1 hematopoietic progenitor cells on pre-metastatic niche formation and in vivo metastasis of breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Du Meng,Min Meng,Anqi Luo,Xin Jing,Guanying Wang,Shangke Huang,Minna Luo,Shan Shao,Xinhan Zhao,Rui Liu

World neurosurgery 120:e611-e616 PubMed30165210

2018

Marked Changes in Blood-Brain Barrier Biomarkers After Direct Bypass Surgery for Moyamoya Angiopathy: Preliminary Study.

Applications

Unspecified application

Species

Unspecified reactive species

Daizo Ishii,Toshinori Matsushige,Takahito Okazaki,Katsuhiro Shinagawa,Shigeyuki Sakamoto,Jumpei Oshita,Kaoru Kurisu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human VE Cadherin ELISA Kit

Sandwich ELISA - Human VE Cadherin ELISA Kit (AB210968)

Sandwich ELISA - Human VE Cadherin ELISA Kit (AB210968)

Sandwich ELISA - Human VE Cadherin ELISA Kit (AB210968)

Sandwich ELISA - Human VE Cadherin ELISA Kit (AB210968)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise