Human VEGF-D ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human VEGF-D in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum samples.
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- Aging 14:4137-41572022LncRNA CRART16/miR-122-5p/FOS axis promotes angiogenesis of gastric cancer by upregulating VEGFD expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunling Zhang,Xiaocong Pang,Lili Lei,Jixin Zhang,Xiaoqian Zhang,Ziyi Chen,Jing Zhu,Yong Jiang,Guowei Chen,Yingchao Wu,Tao Wu,Yisheng Pan,Yucun Liu,Yimin Cui,Xin WangPubMed 35537818
- Cell death & disease 13:2722022Bevacizumab attenuates osteosarcoma angiogenesis by suppressing MIAT encapsulated by serum-derived extracellular vesicles and facilitating miR-613-mediated GPR158 inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBao-Dong Wang,Xiao-Jun Yu,Ji-Chun Hou,Bo Fu,Hao Zheng,Qi-Kun Liu,Shan-Xi Wang,Zheng-Gang Bi,Yang CaoPubMed 35347106
- Experimental and therapeutic medicine 20:1412020Expression and predictive value of HIF-1α and VEGF in patients with burns following treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhufeng Liu et. al.PubMed 33093879
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
348.2 - 10000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 75.7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
Alternative names
FIGF, VEGFD, Vascular endothelial growth factor D, VEGF-D, c-Fos-induced growth factor, FIGF
What's included?
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Human VEGF-D ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human VEGF-D in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
348.2 - 10000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 75.7 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample VEGF-D/serum | n 5 | mean - | SD - | C.V. 2.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample VEGF-D/serum | n 3 | mean - | SD - | C.V. 2.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 88 | Range 85 - 92 % |
Sample type Heparin Plasma | Average % = 78 | Range 74 - 82 % |
Sample type Citrate plasma | Average % = 88 | Range 84 - 92 % |
Sample type Cell culture media | Average % = 101 | Range 98 - 106 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human VEGF-D ELISA Kit (ab233625) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VEGF-D protein in hep plasma, serum, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human VEGF-D with 75.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VEGFD also known as vascular endothelial growth factor D plays a role in angiogenesis and lymphangiogenesis by interacting with VEGF receptors. This protein has a molecular mass of approximately 32 kDa. It is typically expressed in the lungs heart skeletal muscle and small intestine. VEGFD undergoes several proteolytic processes to gain full activity highlighting its functional complexity in the body.
- Biological function summary
Vascular endothelial growth factor D promotes the proliferation and migration of endothelial cells facilitating vascular formation and remodeling. It forms part of the VEGF protein family which is known for its involvement in various vascular processes. The protein's action is largely mediated through its binding to VEGFR-2 and VEGFR-3. This binding activates downstream signaling pathways that contribute to the growth and maintenance of blood and lymphatic vessels.
- Pathways
The functioning of VEGFD is important in the vascular endothelial growth factor signaling pathway and the PI3K-Akt signaling pathway. These pathways are essential for the regulation of cellular processes such as survival growth and motility of endothelial cells. VEGFD is closely related to other members of the VEGF family including VEGFA and VEGFC which similarly interact with VEGF receptors to regulate vascular development and function.
- Associated diseases and disorders
Abnormal levels or activity of VEGFD can lead to pathological conditions such as cancer and lymphedema. In several cancers VEGFD is implicated in tumor angiogenesis aiding the tumor in acquiring nutrients and oxygen. Furthermore in lymphedema issues related to lymphatic vessel development can involve VEGFD alongside VEGFC contributing to fluid retention and tissue swelling. These associations highlight the importance of understanding VEGFD's functions in various pathological contexts.
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4 product images
Sandwich ELISA - Human VEGF-D ELISA Kit (ab233625)
Example of human VEGF-D standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human VEGF-D ELISA Kit (ab233625)
Interpolated concentrations of native VEGF-D in human serum and plasma samples.
The concentrations of VEGF-D were measured in duplicates, interpolated from the VEGF-D standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, and plasma (heparin) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF-D concentration was determined to be 2,105 pg/mL in neat serum, 1,349 pg/mL in neat plasma (citrate) and 1,232 pg/mL in neat plasma (heparin). Native signal is undetectable in RPMI cell culture supernatant containing 10% bovine serum (not shown).
Sandwich ELISA - Human VEGF-D ELISA Kit (ab233625)
Interpolated concentrations of spike VEGF-D in human serum, plasma, and cell culture media samples.
The concentrations of VEGF-D were measured in duplicates, interpolated from the VEGF-D standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, plasma (heparin) neat, and RPMI cell culture media containing 10% FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Native signal is undetectable in RPMI cell culture supernatant containing 10% bovine serum (not shown).
Sandwich ELISA - Human VEGF-D ELISA Kit (ab233625)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF-D concentration was determined to be 2,193 pg/mL with a range of 956.9 – 3,270 pg/mL.
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