AB100663

Human VEGF ELISA Kit

Name: Human VEGF ELISA Kit (ab100663) | Abcam
Brand: Abcam Limited
SKU: AB100663
Price: 780 USD
Availability: InStock

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(25 Publications)

Human VEGF ELISA Kit is a Sandwich ELISA for the measurement of Human VEGF in Human in Cell/Tissue Extracts samples.

View Alternative Names

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

3 Images

Sandwich ELISA - Human VEGF ELISA Kit (AB100663)

sELISA

Lab

Sandwich ELISA - Human VEGF ELISA Kit (AB100663)

Hu VEGF in various fluids showing quantity (pg) per mL of tested sample

sELISA

Lab

Sandwich ELISA - Human VEGF ELISA Kit (AB100663)

Standard curve : mean of duplicates (+/-SD) with background readings subtracted

sELISA

Lab

Sandwich ELISA - Human VEGF ELISA Kit (AB100663)

Hu VEGF measured in cell lysates showing quantity (pg) per million cells tested

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

8.23 - 6000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's VEGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF in cell lysates and tissue lysates.

This assay employs an antibody specific for Human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Recovery

[ { "sample": "Cell culture extracts", "range": "90 - 107 %", "average": "= 96.79" }, { "sample": "Tissue Extracts", "range": "89 - 106 %", "average": "= 95.58" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100663-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100663-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100663-CMP04", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB100663-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100663-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human VEGF Standard (lyophilized)", "number":"AB100663-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human VEGF", "number":"AB100663-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100663-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100663-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"VEGF Microplate (12 x 8 wells)", "number":"AB100663-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer", "number":"AB100663-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.

Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.

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Product protocols

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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

See full target information VEGFA

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

BMC complementary medicine and therapies 23:186 PubMed37287012

2023

Preclinical studies of toxicity and anti-cholangiocarcinoma activity of the standardized capsule formulation of Atractylodes lancea (Thunb.) DC.

Applications

Unspecified application

Species

Unspecified reactive species

Tullayakorn Plengsuriyakarn,Kanawut Kotawong,Juntra Karbwang,Kesara Na-Bangchang

Bioengineered 13:13622-13631 PubMed35653799

2022

Paeoniflorin alleviates the progression of retinal vein occlusion via inhibiting hypoxia inducible factor-1α/vascular endothelial growth factor/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lingchun Kong,Jingjing Li,Yuqin Yang,Huixin Tang,Hong Zou

Journal of extracellular vesicles 11:e12207 PubMed35398993

2022

A functional corona around extracellular vesicles enhances angiogenesis, skin regeneration and immunomodulation.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Wolf,Rodolphe W Poupardin,Patricia Ebner-Peking,André Cronemberger Andrade,Constantin Blöchl,Astrid Obermayer,Fausto Gueths Gomes,Balazs Vari,Nicole Maeding,Essi Eminger,Heide-Marie Binder,Anna M Raninger,Sarah Hochmann,Gabriele Brachtl,Andreas Spittler,Thomas Heuser,Racheli Ofir,Christian G Huber,Zami Aberman,Katharina Schallmoser,Hans-Dieter Volk,Dirk Strunk

Frontiers in nutrition 9:857256 PubMed35464036

2022

Carvacrol Arrests the Proliferation of Hypopharyngeal Carcinoma Cells by Suppressing Ornithine Decarboxylase and Hyaluronidase Activities.

Applications

Unspecified application

Species

Unspecified reactive species

Kaneez Fatima,Suaib Luqman,Abha Meena

Stem cell research & therapy 11:535 PubMed33308306

2020

Hypoxia-preconditioned adipose-derived stem cells combined with scaffold promote urethral reconstruction by upregulation of angiogenesis and glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Wan,Min-Kai Xie,Huan Xu,Zi-Wei Wei,Hai-Jun Yao,Zhong Wang,Da-Chao Zheng

Stem cells international 2020:2803747 PubMed32377202

2020

Enhanced Effect of IL-1-Activated Adipose-Derived MSCs (ADMSCs) on Repair of Intestinal Ischemia-Reperfusion Injury via COX-2-PGE Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Liu,Yi Ren He,Shao Jun Liu,Lei Hu,Li Chuang Liang,Dong Liang Liu,Lin Liu,Zhi Qiang Zhu

Clinical & experimental metastasis 37:199-207 PubMed31768815

2019

Bevacizumab suppresses the growth of established non-small-cell lung cancer brain metastases in a hematogenous brain metastasis model.

Applications

Unspecified application

Species

Unspecified reactive species

Chinami Masuda,Masamichi Sugimoto,Daiko Wakita,Makoto Monnai,Chisako Ishimaru,Ryo Nakamura,Mari Kinoshita,Keigo Yorozu,Mitsue Kurasawa,Osamu Kondoh,Kaname Yamamoto

Drug design, development and therapy 13:3383-3389 PubMed31576112

2019

Effect Of Bevacizumab On Growth Of Human Nasal Polyposis In Vitro; An Off-Label Use Of Anti-Angiogenic Agent For Nasal Polyposis Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Shadman Nemati,Faeze Keihanian,Amin Saeidinia,Mahdi Bakhshaei

Biochemical and biophysical research communication 507:185-192 PubMed30454892

2018

The positive feedback between lncRNA TNK2-AS1 and STAT3 enhances angiogenesis in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Wang,Dongmei Han,Liming Pan,Jing Sun

Journal of cellular physiology 234:3425-3435 PubMed30387128

2018

Decellularized breast matrix as bioactive microenvironment for in vitro three-dimensional cancer culture.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Jin,Gang Liu,Shubin Li,Haihan Yuan,Zhizhong Yun,Wenqi Zhang,Shu Zhang,Yanfeng Dai,Yuzhen Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human VEGF ELISA Kit

Sandwich ELISA - Human VEGF ELISA Kit (AB100663)

Sandwich ELISA - Human VEGF ELISA Kit (AB100663)

Sandwich ELISA - Human VEGF ELISA Kit (AB100663)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (25)

Product promise