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AB222510

Human VEGF ELISA Kit

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(1 Review)

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(33 Publications)

Human VEGF ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human vascular endothelial growth factor (VEGF) with a sensitivity of 2.7 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA with a 450 nm readout; compatible with any standard plate reader
- Different formats for different needs including 384-well plate for higher throughput
- Design your own immunoassay: we also offer the cojugation-ready antibody pair
- Cited in over 30 publications

View Alternative Names

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

22 Images
ELISA - Human VEGF ELISA Kit (AB222510)
  • ELISA

Supplier Data

ELISA - Human VEGF ELISA Kit (AB222510)
Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Example of human VEGF standard curve in 1X Cell Extraction Buffer PTR.

The VEGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Linearity of dilution.

Native VEGF was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS + 2X Enhancer and in Sample Diluent NS + 1X Enhancer, see Sample Preparation section for details. Cell culture supernatants are represented by SN.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Comparison of VEGF in PHA-M stimulated and unstimulated human PBMC cell culture supernatants stimulated for different durations.

Human PBMC (seeded at 10x106/mL) were cultured for 2 or 5 days in the presence or absence of 1.5% PHA-M. The concentrations of VEGF were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the VEGF standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean VEGF concentration was determined to be 34.76 pg/mL in 2 Day PHA-M stimulated PBMC cell culture supernatant, 393.7 pg/mL in 5 Day PHA-M stimulated PBMC cell culture supernatant, and undetectable in unstimulated PBMC cell culture supernatant at both 2 and 5 days (not shown).

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Interpolated concentrations of native VEGF in human serum and plasma samples.

The concentrations of VEGF were measured in duplicates, interpolated from the VEGF standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF concentration was determined to be 268.9 pg/mL in neat serum, 123.9 pg/mL in neat plasma (citrate), 133.8 pg/mL in neat plasma (heparin), and 95.76 pg/mL in neat plasma (EDTA).

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Example of human VEGF standard curve in Sample Diluent NS + 1X Enhancer.

The VEGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF concentration was determined to be 235.0 pg/mL with a range of 81.10 – 993.3 pg/mL.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native VEGF was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Recombinant human VEGF was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS + 2X Enhancer and in Sample Diluent NS + 1X Enhancer, see Sample Preparation section for details.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Example of human VEGF standard curve in Sample Diluent NS + 1X Enhancer.

The VEGF standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Interpolated concentrations of native VEGF in human breast milk (de-fatted), saliva, and urine samples.

The concentrations of VEGF were measured in duplicates, interpolated from the VEGF standard curves and corrected for sample dilution. Undiluted samples are as follows : breast milk 2%, saliva 12.5%, and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF concentration was determined to be 21487 pg/mL in neat human breast milk (de-fatted), 3712 pg/mL in neat human saliva, and 262.0 pg/mL in neat human urine.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Interpolated concentrations of native VEGF in PC-3 cell extract (1 Day), PC-3 cell extract (2 Day), HepG2 cell extract, A549 cell extract, and MDA-MB-435S cell extract samples based on a 300 μg/mL extract load. The concentrations of VEGF were measured in duplicate and interpolated from the VEGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF concentration was determined to be 86.65 pg/mL in PC-3 cell extract (1 Day), 149.6 pg/mL in PC-3 cell extract (2 Day), 127.7 pg/mL in HepG2 cell extract, 241.7 pg/mL in A549 cell extract, and 454.5 pg/mL in MDA-MB-435S cell extract.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Interpolated concentrations of native VEGF in cell culture supernatant samples.

The concentrations of VEGF were measured in duplicates, interpolated from the VEGF standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 5%, A431 5%, MDA-MB-435S 12.5%, PC-3 20%, A549 50%, and PBMC 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF concentration was determined to be 5027 pg/mL in neat HepG2 supernatant, 7356 pg/mL in neat A431 supernatant (4 Day), 5918 pg/mL in neat MDA-MB-435S supernatant, 1495 pg/mL in neat PC-3 (2 Day), 603.4 pg/mL in neat A549 supernatant, and 405.3 pg/mL in neat PBMC supernatant (PHA-M, 5 Day).

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Assay sensitivity.

The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Serial dilutions of recombinant human VEGF189 and VEGF121 were prepared and assayed in parallel with recombinant VEGF165.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Other species reactivity was determined by measuring a 500 pg/mL protein load of various species recombinant VEGF proteins.

Protein concentrations were interpolated from the human standard curve, and graphing the interpolated concentrations (mean +/- SD, n=2).

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Linearity of dilution.

Native VEGF was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS + 2X Enhancer and in Sample Diluent NS + 1X Enhancer, see Sample Preparation section for details. Cell culture supernatants are represented by SN.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Example of human VEGF standard curve in 1X Cell Extraction Buffer PTR.

The VEGF standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Linearity of dilution.

Native VEGF was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Lab

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Example of human VEGF standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Lab

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Example of human VEGF standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Lab

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Interpolated concentration of native VEGF was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 12.5% saliva. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)
  • sELISA

Lab

Sandwich ELISA - Human VEGF ELISA Kit (AB222510)

Interpolated concentration of native VEGF was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 500 µg/mL A549 cell extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Saliva, Urine, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.7 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human VEGF ELISA Kit ab222510 is a rapid single-wash 90-min ELISA kit to measure Human VEGF in cerebral spinal fluid, saliva, urine, cell culture extracts, cell culture supernatant, milk, serum and plasma. This SimpleStep ELISA® sensitivity is 2.7 pg/mL.

How the assay works

Human VEGF SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Serial dilutions of recombinant human VEGFR1, VEGFR2, and VEGFR3 were prepared starting at 4 ng/mL and tested for interference. No interference was observed.
This VEGF immunoassay is calibrated against a highly purified human VEGF. The NIBSC/WHO unclassified purified human VEGF preparation 02/286 was evaluated in this kit. The dose response curve of the unclassified standard 02/286 parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep human VEGF kit to approximate NIBSC 02/286 units, use the equation below.
NIBSC 02/286 approximate value (IU/mL) = 0.0011 x SimpleStep human VEGF value (pg/mL).

Human VEGF ELISA Kit ab222510 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human VEGF ELISA kit ab222510

Human VEGF ELISA kit ab222510 has been used to assess endometrial development and receptivity(1) and to study VEGF as a predictor of arrhythmia in acute coronary syndrome patient(2).

References:

(1) Pin-Yao Lin et al. 2023, PMID:37763186,
(2) Bin Li et al. 2023, PMID: 3735044

Save your precious samples

Our 384-well Human VEGF ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human IL-12 p70 Antibody Pair - BSA and Azide free ab244013
- Anti-VEGFA antibody [EPR24630-77] - BSA and Azide free (Capture) ab312861
- Anti-VEGFA antibody [EPR24630-90] - BSA and Azide free (Detector) ab312862

Related and recommended products

Human VEGF ELISA kit ab222510 is commonly used to measure VEGF as a marker of angiogenesis.

Other ELISA kits to measure growth factors are:

- Human EGF ELISA kit ab217772
- Human PEDF ELISA kit ab246535
- Human HGF ELISA kit ab275901

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "80 - 94 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "81 - 89 %", "average": "= 86" }, { "sample": "Cerebral Spinal Fluid", "range": "89 - 95 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "95 - 107 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "95 - 99 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "84 - 92 %", "average": "= 89" }, { "sample": "Milk", "range": "91 - 98 %", "average": "= 95" }, { "sample": "Saliva", "range": "105 - 118 %", "average": "= 109" }, { "sample": "Serum", "range": "98 - 116 %", "average": "= 109" }, { "sample": "Urine", "range": "91 - 102 %", "average": "= 95" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
See full target information VEGFA
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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:7639 PubMed37993431

2023

Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Ye He,Shujie Yang,Pengzhan Liu,Ke Li,Ke Jin,Ryan Becker,Jinxin Zhang,Chuanchuan Lin,Jianping Xia,Zhehan Ma,Zhiteng Ma,Ruoyu Zhong,Luke P Lee,Tony Jun Huang

Journal of personalized medicine 13: PubMed37763186

2023

Factors Affecting the Potential Efficacy of Intrauterine Platelet-Rich Plasma Infusion on Thin Endometrium in Women with Recurrent Implantation Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Pin-Yao Lin,Chun-I Lee,Yi-Chun Chen,En-Hui Cheng,Chun-Chia Huang,Chung-I Chen,Tsung-Hsien Lee,Yu-Jen Lee,Maw-Sheng Lee

Scientific reports 13:15046 PubMed37699959

2023

miRNA-126a plays important role in myoblast and endothelial cell interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Bartosz Mierzejewski,Maria Anna Ciemerych,Wladyslawa Streminska,Katarzyna Janczyk-Ilach,Edyta Brzoska

Experimental biology and medicine (Maywood, N.J.) 248:685-690 PubMed37350444

2023

Predictive value of serum HIF-1α and VEGF for arrhythmia in acute coronary syndrome patients.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Li,Qiuting Feng,Cheng Yu,Jun Yang,Xian Qin,Xing Li,Jianing Cao,Xin Xu,Chenjian Yang,Yan Jin

Functional & integrative genomics 23:164 PubMed37198330

2023

IL-1RA inhibits esophageal carcinogenesis and lymphangiogenesis via downregulating VEGF-C and MMP9.

Applications

Unspecified application

Species

Unspecified reactive species

Zhimin Shen,Peipei Zhang,Weiguang Zhang,Fei Luo,Hui Xu,Shuchen Chen,Mingqiang Kang

International journal of molecular sciences 24: PubMed37175778

2023

Intraocular Pressure-Lowering and Retina-Protective Effects of Exosome-Rich Conditioned Media from Human Amniotic Membrane Stem Cells in a Rat Model of Glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hye-Rim Seong,Chan Ho Noh,Sangryong Park,Sumin Cho,Seok-Jin Hong,Ah-Young Lee,Dongho Geum,Soon-Cheol Hong,Dongsun Park,Tae Myoung Kim,Ehn-Kyoung Choi,Yun-Bae Kim

Investigative ophthalmology & visual science 64:23 PubMed36820679

2023

Macular Edema in Central Retinal Vein Occlusion Correlates With Aqueous Fibrinogen Alpha Chain.

Applications

Unspecified application

Species

Unspecified reactive species

Lasse Jørgensen Cehofski,Kentaro Kojima,Natsuki Kusada,Maja Rasmussen,Danson Vasanthan Muttuvelu,Jakob Grauslund,Henrik Vorum,Bent Honoré

International journal of environmental research and public health 20: PubMed36834246

2023

Acute Responses to High-Intensity Back Squats with Bilateral Blood Flow Restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Bjoern Hornikel,Keith S Saffold,Michael R Esco,Jacob A Mota,Michael V Fedewa,Stefanie A Wind,Tiffany L Adams,Lee J Winchester

Oncoimmunology 11:1965317 PubMed36524211

2022

Mesenchymal stem cells shuttling miR-503 extracellular vesicles enhance glioma immune escape.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Song Wang,Xiao-Jun Yu,Kang Wei,Shan-Xi Wang,Qi-Kun Liu,Ying-Guang Wang,Han Li,Cheng Huang

Journal of controlled release : official journal of the Controlled Release Society 352:879-892 PubMed36370875

2022

Porous scaffold for mesenchymal cell encapsulation and exosome-based therapy of ischemic diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Czosseck,Max M Chen,Helen Nguyen,Annette Meeson,Chuan-Chih Hsu,Chien-Chung Chen,Thomashire A George,Shu-Chian Ruan,Yuan-Yuan Cheng,Po-Ju Lin,Patrick C H Hsieh,David J Lundy
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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