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AB119613

Human VEGF R1 ELISA Kit (FLT1)

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(3 Publications)

Human VEGF R1 ELISA Kit (FLT1) is a Sandwich (quantitative) ELISA for the measurement of Human VEGF R1 (FLT1) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

FLT, FRT, VEGFR1, FLT1, Vascular endothelial growth factor receptor 1, VEGFR-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor, FLT-1

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Sandwich ELISA - Human VEGF R1 ELISA Kit (FLT1) (AB119613)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF R1 ELISA Kit (FLT1) (AB119613)

Representative standard curve using ab119613

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 30 pg/mL

Range

156 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human soluble VEGF R1 (FLT1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human soluble VEGF R1 (FLT1) in cell culture supernatants, serum and plasma (EDTA).

A VEGF R1 (FLT1) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for VEGF R1 (FLT1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution The density of yellow coloration is directly proportional to the Human VEGF R1 (FLT1) amount of sample captured in plate.

Get higher sensitivity in only 90 minutes with Human VEGF R1 ELISA Kit (FLT1) (ab195210) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1352 pg/mL", "standardDeviation": "131.14", "coefficientOfVariability": "= 9.7" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4420 pg/mL", "standardDeviation": "203.32", "coefficientOfVariability": "= 4.6" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "237 pg/mL", "standardDeviation": "9.71", "coefficientOfVariability": "= 4.1" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "240 pg/mL", "standardDeviation": "11.28", "coefficientOfVariability": "= 4.7" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "4316 pg/mL", "standardDeviation": "202.85", "coefficientOfVariability": "= 4.7" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1369 pg/mL", "standardDeviation": "106.78", "coefficientOfVariability": "= 7.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119613-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119613-CMP09", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human VEGF Receptor 1 antibody", "number":"AB119613-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human VEGF Receptor 1 antibody Microplate (12 x 8 wells)", "number":"AB119613-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119613-CMP03", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant Human VEGF Receptor 1 standard", "number":"AB119613-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119613-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119613-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119613-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119613-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119613-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGF Receptor 1 also known as FLT-1 is a protein that plays a critical role in the regulation of angiogenesis. Its mass is approximately 180 kDa. FLT-1 is a receptor tyrosine kinase expressed mainly on the surface of endothelial cells but also in monocytes and macrophages. The receptor binds vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) which initiates a series of signaling cascades important for vascular development and function.
Biological function summary

FLT-1 mediates important cellular responses such as cell migration and proliferation. Although it often functions in conjunction with other receptors like VEGFR-2 FLT-1 has unique binding affinities that modulate the availability of VEGF for other receptors. FLT-1 proteins as part of complex signaling networks actively contribute to maintaining vascular homeostasis and regulating blood vessel permeability.

Pathways

FLT-1 is an important component in the VEGF signaling pathway and also part of the PlGF/VEGF pathway. The receptor serves as a decoy to regulate the amount of VEGF interaction with VEGFR-2 influencing endothelial cell function and vascular permeability. By sequestering VEGF FLT-1 helps maintain the balance in this angiogenic signaling network interacting with proteins like VEGF PlGF and the anti-VEGF agents that target these pathways.

FLT-1 is implicated in cancer and diabetic retinopathy. Overexpression or dysregulation of FLT-1 can contribute to tumor angiogenesis which can enhance tumor growth and metastasis. In diabetic retinopathy abnormal FLT-1 activity affects retinal blood vessels leading to vision impairment. Similar interactions involve VEGFR-2 and other downstream effectors emphasizing the potential of FLT-1 as a therapeutic target for conditions associated with excessive angiogenesis and vascular dysfunction.
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Product protocols

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Acts as a positive regulator of postnatal retinal hyaloid vessel regression (By similarity). May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1 (PubMed : 16685275).. Isoform 1. Phosphorylates PLCG.. Isoform 2. May function as decoy receptor for VEGFA.. Isoform 3. May function as decoy receptor for VEGFA.. Isoform 4. May function as decoy receptor for VEGFA.. Isoform 7. Has a truncated kinase domain; it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion.
See full target information FLT1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed35883694

2022

Maternal Fibroblast Growth Factor 21 Levels Decrease during Early Pregnancy in Normotensive Pregnant Women but Are Higher in Preeclamptic Women-A Longitudinal Study.

Applications

Unspecified application

Species

Unspecified reactive species

Julieth Daniela Buell-Acosta,Maria Fernanda Garces,Arturo José Parada-Baños,Edith Angel-Muller,Maria Carolina Paez,Javier Eslava-Schmalbach,Franklin Escobar-Cordoba,Sofia Alexandra Caminos-Cepeda,Ezequiel Lacunza,Justo P Castaño,Rubén Nogueiras,Carlos Dieguez,Ariel Iván Ruiz-Parra,Jorge Eduardo Caminos

PloS one 10:e0131013 PubMed26121675

2015

Maternal Serum Meteorin Levels and the Risk of Preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

María F Garcés,Elizabeth Sanchez,Luisa F Cardona,Elkin L Simanca,Iván González,Luis G Leal,José A Mora,Andrés Bedoya,Juan P Alzate,Ángel Y Sánchez,Javier H Eslava-Schmalbach,Roberto Franco-Vega,Mario O Parra,Ariel I Ruíz-Parra,Carlos Diéguez,Rubén Nogueiras,Jorge E Caminos

Oncology reports 32:1385-94 PubMed25109488

2014

Mead acid inhibits the growth of KPL-1 human breast cancer cells in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichi Kinoshita,Katsuhiko Yoshizawa,Kei Hamazaki,Yuko Emoto,Takashi Yuri,Michiko Yuki,Nobuaki Shikata,Hiroshi Kawashima,Airo Tsubura
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