Human VEGF R1 (FLT1) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human VEGF R1 (FLT1) in Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Monkey, Human
- Range
156.25 - 10000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 53.86 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Acts as a positive regulator of postnatal retinal hyaloid vessel regression (By similarity). May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1 (PubMed:16685275).Isoform 1Phosphorylates PLCG.Isoform 2May function as decoy receptor for VEGFA.Isoform 3May function as decoy receptor for VEGFA.Isoform 4May function as decoy receptor for VEGFA.Isoform 7Has a truncated kinase domain; it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion.
Alternative names
FLT, FRT, VEGFR1, FLT1, Vascular endothelial growth factor receptor 1, VEGFR-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor, FLT-1, FLT
What's included?
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Human VEGF R1 (FLT1) ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human VEGF R1 (FLT1) in Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Monkey, Human
- Range
156.25 - 10000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 53.86 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 8 | mean - | SD - | C.V. 3.06 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 5.51 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 106 | Range 102 - 114 % |
Sample type Serum | Average % = 89 | Range 86 - 95 % |
Sample type EDTA Plasma | Average % = 93 | Range 90 - 95 % |
Sample type Saliva | Average % = 114 | Range 111 - 119 % |
Sample type Urine | Average % = 96 | Range 93 - 99 % |
Sample type Heparin Plasma | Average % = 86 | Range 84 - 88 % |
Sample type Citrate plasma | Average % = 92 | Range 91 - 94 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human VEGF R1 (FLT1) ELISA Kit (ab289705) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human VEGF R1 (FLT1) in serum, plasma (heparin), plasma (EDTA), plasma (citrate), cell culture supernatant, saliva, and urine samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human VEGF R1 (FLT1) with 53.86 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VEGF Receptor 1 also known as FLT-1 is a protein that plays a critical role in the regulation of angiogenesis. Its mass is approximately 180 kDa. FLT-1 is a receptor tyrosine kinase expressed mainly on the surface of endothelial cells but also in monocytes and macrophages. The receptor binds vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) which initiates a series of signaling cascades important for vascular development and function.
- Biological function summary
FLT-1 mediates important cellular responses such as cell migration and proliferation. Although it often functions in conjunction with other receptors like VEGFR-2 FLT-1 has unique binding affinities that modulate the availability of VEGF for other receptors. FLT-1 proteins as part of complex signaling networks actively contribute to maintaining vascular homeostasis and regulating blood vessel permeability.
- Pathways
FLT-1 is an important component in the VEGF signaling pathway and also part of the PlGF/VEGF pathway. The receptor serves as a decoy to regulate the amount of VEGF interaction with VEGFR-2 influencing endothelial cell function and vascular permeability. By sequestering VEGF FLT-1 helps maintain the balance in this angiogenic signaling network interacting with proteins like VEGF PlGF and the anti-VEGF agents that target these pathways.
- Associated diseases and disorders
FLT-1 is implicated in cancer and diabetic retinopathy. Overexpression or dysregulation of FLT-1 can contribute to tumor angiogenesis which can enhance tumor growth and metastasis. In diabetic retinopathy abnormal FLT-1 activity affects retinal blood vessels leading to vision impairment. Similar interactions involve VEGFR-2 and other downstream effectors emphasizing the potential of FLT-1 as a therapeutic target for conditions associated with excessive angiogenesis and vascular dysfunction.
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4 product images
Sandwich ELISA - Human VEGF R1 (FLT1) ELISA Kit (ab289705)
Human VEGF R1 standard curve comparison.
Standard curve comparison between the original Human VEGF R1 SimpleStep ELISA (ab195210) and current Human VEGF R1 SimpleStep ELISA (ab289705).
Sandwich ELISA - Human VEGF R1 (FLT1) ELISA Kit (ab289705)
Interpolated concentrations of VEGF R1 in normal human serum donors.
Serum of six individual healthy human donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean VEGF R1 concentration was determined to be 528 pg/mL with a range of 373 – 924 pg/mL.
Sandwich ELISA - Human VEGF R1 (FLT1) ELISA Kit (ab289705)
Interpolated concentrations of human VEGF R1 in HUVEC cell culture supernatant and saliva.
Interpolated concentration of native VEGF R1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: HUVEC cell culture supernatant 25% and saliva 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human VEGF R1 (FLT1) ELISA Kit (ab289705)
Example of human VEGF R1 standard curve in Sample Diluent NS.
Example of human VEGF R1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
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