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AB213476

Human VEGF Receptor 2 ELISA Kit

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(4 Publications)

Human VEGF Receptor 2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human VEGF Receptor 2 with a sensitivity of 10 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD309, FLK1, VEGFR2, KDR, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, FLK-1

5 Images
Sandwich ELISA - Human VEGF Receptor 2 ELISA Kit (AB213476)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF Receptor 2 ELISA Kit (AB213476)

Interpolated concentrations of native VEGF Receptor 2 in human plasma heparin, and spiked recombinant VEGF Receptor 2 in HUVEC culture supernatant samples.

The concentrations of VEGF Receptor 2 were measured in duplicate, interpolated from the VEGF Receptor 2 standard curve and corrected for sample dilution. Undiluted samples are as follows : plasma (heparin) 12.5% and HUVEC culture supernatant 80%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF Receptor 2 concentration was determined to be 44 ng/mL in plasma (heparin). Native signal of HUVEC culture supernatant at 80% was determined to be 0.4 ng/mL.

Sandwich ELISA - Human VEGF Receptor 2 ELISA Kit (AB213476)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF Receptor 2 ELISA Kit (AB213476)

Example of human VEGF Receptor 2 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human VEGF Receptor 2 ELISA Kit (AB213476)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF Receptor 2 ELISA Kit (AB213476)

Serum from ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF Receptor 2 concentration was determined to be 2.37 ng/mL with a range of 1.19 – 3.23 ng/mL.

Sandwich ELISA - Human VEGF Receptor 2 ELISA Kit (AB213476)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF Receptor 2 ELISA Kit (AB213476)

Interpolated concentrations of native VEGF Receptor 2 in human serum, plasma citrate, and plasma EDTA samples.

The concentrations of VEGF Receptor 2 were measured in duplicate, interpolated from the VEGF Receptor 2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 7.5%, plasma (citrate) 7.5%, and plasma (EDTA) 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF Receptor 2 concentration was determined to be 60 ng/mL in serum, 42 ng/mL in plasma (citrate) and 49 ng/mL in plasma (EDTA).

Sandwich ELISA - Human VEGF Receptor 2 ELISA Kit (AB213476)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGF Receptor 2 ELISA Kit (AB213476)

Example of human VEGF Receptor 2 standard curve in Sample Diluent 3.75BS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10 pg/mL

Range

117 - 7500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human VEGF Receptor 2 ELISA Kit (ab213476) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VEGF Receptor 2 protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human VEGF Receptor 2 with 10 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

VEGF R2 (vascular endothelial growth factor receptor 2) is 1356 amino acids long and is a class III receptor tyrosine kinase. VEGF R2 acts as a cell surface receptor for VEGFA, VEGFC, and VEGFD, where binding of these VEGFs to VEGF R2 activates several signaling cascades, including activation of PLCG1 (leading to the activation of protein kinase C), the MAP kinase signaling pathways, and the AKT1 signaling pathway. Furthermore, VEGF R2 plays an essential role in the regulation of angiogenesis, vascular development and permeability, embryonic hematopoiesis, and reorganization of the actin cytoskeleton. Finally, VEGF R2 is a major growth factor for endothelial cells and is the main facilitator of VEGF-induced endothelial proliferation, differentiation, and migration.

The standard is affinity purified.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "95 - 98 %", "average": "= 97" }, { "sample": "Serum", "range": "94 - 97 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "89 - 98 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "95 - 99 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "109 - 122 %", "average": "= 114" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGF Receptor 2 also known as KDR or Flk-1 is a receptor tyrosine kinase important for mediating the effects of vascular endothelial growth factor (VEGF). This protein has a mass of approximately 150 kDa. It mostly expresses in vascular endothelial cells playing a primary role in the formation of blood vessels. The receptor can also be found in various non-endothelial tissues though in smaller amounts indicating its broad functional roles outside of the vascular system.
Biological function summary

VEGF Receptor 2 is important for angiogenesis and vascular permeability. It is part of the VEGF receptor family which also includes VEGF Receptors 1 and 3 forming a complex interaction network. Upon binding with VEGF it phosphorylates key proteins to initiate signals that regulate endothelial cell migration proliferation and survival. The receptor's function is critical for normal developmental processes and wound healing as well.

Pathways

VEGF Receptor 2 is especially important within the angiogenesis and vascular permeability pathways. It plays a central role in the VEGF signaling pathway alongside proteins like VEGF A which is the primary ligand for the receptor. The signaling through this receptor modulates various downstream proteins through phosphorylation linking it to pathways that control cellular responses essential for blood vessel formation and maintenance.

VEGF Receptor 2 is significantly linked with cancer and age-related macular degeneration. In the context of cancer the overexpression or dysregulation of VEGF and its receptors including VEGF Receptor 2 often leads to enhanced tumor angiogenesis promoting tumor growth and metastasis. Additionally the receptor interacts with other proteins in pathological states such as CD309 in endothelial cell-based assays which are used for research like flow cytometry and ELISA to monitor VEGF Receptor 2 activity and its impact on diseases.
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Product protocols

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
See full target information KDR
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 15: PubMed36297374

2022

Identification of Novel Cyanopyridones and Pyrido[2,3-]Pyrimidines as Anticancer Agents with Dual VEGFR-2/HER-2 Inhibitory Action: Synthesis, Biological Evaluation and Molecular Docking Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Tarfah Al-Warhi,Al-Aliaa M Sallam,Loah R Hemeda,Mahmoud A El Hassab,Nada Aljaeed,Ohoud J Alotaibi,Ahmed S Doghish,Mina Noshy,Wagdy M Eldehna,Mona H Ibrahim

Polymers 14: PubMed36015608

2022

Synthesis of Silver Nanocomposite Based on Carboxymethyl Cellulose: Antibacterial, Antifungal and Anticancer Activities.

Applications

Unspecified application

Species

Unspecified reactive species

Salem S Salem,Amr H Hashem,Al-Aliaa M Sallam,Ahmed S Doghish,Abdulaziz A Al-Askar,Amr A Arishi,Amr M Shehabeldine

Pharmaceuticals (Basel, Switzerland) 15: PubMed35337128

2022

Design, Green Synthesis and Tailoring of Vitamin E TPGS Augmented Niosomal Nano-Carrier of Pyrazolopyrimidines as Potential Anti-Liver and Breast Cancer Agents with Accentuated Oral Bioavailability.

Applications

Unspecified application

Species

Unspecified reactive species

Kurls E Anwer,Nour E A Abd El-Sattar,Marium M Shamaa,Mohamed Y Zakaria,Botros Y Beshay

Biomedical reports 14:37 PubMed33692900

2021

Circulating vascular endothelial growth factor receptor 2 levels and their association with lipid abnormalities in patients on hemodialysis.

Applications

Unspecified application

Species

Unspecified reactive species

Leszek Niepolski,Hanna Drzewiecka,Wojciech Warchoł
View all publications
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