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AB119566

Human VEGFA ELISA Kit

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(26 Publications)

Human VEGFA ELISA Kit is a sandwich ELISA designed to quantify Human VEGFA with a sensitivity of 7.9 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 15.6 - 1000 pg/mL
- Cited in over 20 publications

View Alternative Names

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

1 Images
Sandwich ELISA - Human VEGFA ELISA Kit (AB119566)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGFA ELISA Kit (AB119566)

Representative Standard Curve using ab119566.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 7.9 pg/mL

Range

15.6 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human VEGFA ELISA Kit ab119566 is a sandwich ELISA to measure Human VEGFA in serum, plasma, cell culture supernatant with a sensitivity of 7.9 pg/ml.

How the assay works

VEGFA specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Human VEGFA detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of VEGFA captured on the plate.

Produced using a non-baculovirus Escherichia coli expression system.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human VEGFA ELISA Kit ab119566 protocol summary

1. Add standard or sample to appropriate wells. Incubate the plate
2. Wash and add Biotin-Conjugate anti-human VEGFA polyclonal antibody to appropriate wells. Incubate the plate.
3. Wash and add prepared Streptavidin-HRP Conjugate to appropriate wells. Incubate at room temperature
4. Wash and add TMB Substrate to each well
5. Add Stop Solution to each well. Read immediately
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "88 - 98 %", "average": null }, { "sample": "Serum", "range": "81 - 88 %", "average": null } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119566-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB119566-CMP10", "productcode":"" }, { "size":"1 x 5 mL", "name":"20X Assay Buffer Concentrate", "number":"AB119566-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent", "number":"AB119566-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human VEGFA coated microplate (12 x 8 wells)", "number":"AB119566-CMP04", "productcode":"" }, { "size":"6 x 1 Unit", "name":"Adhesive Films", "number":"AB119566-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human VEGFA Standard Lyophilized", "number":"AB119566-CMP05", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotin-Conjugate anti-human VEGFA polyclonal antibody", "number":"AB119566-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB119566-CMP09", "productcode":"" }, { "size":"1 x 150 µL", "name":"Streptavidin-HRP", "number":"AB119566-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution (1M Phosphoric acid)", "number":"AB119566-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
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Product protocols

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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
See full target information VEGFA
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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 16:2197-2211 PubMed40302796

2025

Extracellular vesicles-miR-205-5p inhibits lymphatic metastasis in pancreatic cancer through diffusely downregulating VEGFA.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyang Wang,Cheng Qin,Yutong Zhao,Bangbo Zhao,Zeru Li,Tianyu Li,Xiangyu Zhang,Weibin Wang

Journal of virology 99:e0230924 PubMed40277358

2025

Ubiquitin-dependent proteasomal degradation of small hepatitis B virus surface antigen mediated by TRIM21 and antagonized by OTUD4.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxiang Wu,Zhihan Chen,Zhengqian Zhang,Jing Xu,Hang Li,Mengxian Lin,Wenjie Xie,Yan Chen,Xinjian Lin,Xu Lin

CNS neuroscience & therapeutics 30:e70173 PubMed39727329

2024

Annexin A1 Mitigates Blood-Brain Barrier Disruption in a Sepsis-Associated Encephalopathy Model by Enhancing the Expression of Occludin and Zonula Occludens-1 (ZO-1).

Applications

Unspecified application

Species

Unspecified reactive species

Yao Li,Fang Zhou,Jiyue You,Xinran Gong

Molecular carcinogenesis 63:2174-2189 PubMed39092767

2024

m6A modification of VEGFA mRNA by RBM15/YTHDF2/IGF2BP3 contributes to angiogenesis of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxin Xu,Shuxiang Wu,Yi Zhang,Weijie Fan,Xinjian Lin,Kunqi Chen,Xu Lin

Open life sciences 18:20220670 PubMed38239497

2024

Fractalkine modulates pulmonary angiogenesis and tube formation by modulating CX3CR1 and growth factors in PVECs.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Liao,Xianwu Yang,Jiejie Yang,Jingjing Xiao,Xuyang Liu,Yingquan Zhuo,Jiafei Yang,Huajian Gu

Cell stem cell 30:1640-1657.e8 PubMed38029740

2023

VEGFA mRNA-LNP promotes biliary epithelial cell-to-hepatocyte conversion in acute and chronic liver diseases and reverses steatosis and fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Fatima Rizvi,Yu-Ri Lee,Ricardo Diaz-Aragon,Pushpinder S Bawa,Juhoon So,Rodrigo M Florentino,Susan Wu,Arianna Sarjoo,Emily Truong,Anna R Smith,Feiya Wang,Elissa Everton,Alina Ostrowska,Kyounghwa Jung,Ying Tam,Hiromi Muramatsu,Norbert Pardi,Drew Weissman,Alejandro Soto-Gutierrez,Donghun Shin,Valerie Gouon-Evans

Journal of experimental & clinical cancer research : CR 42:302 PubMed37968723

2023

Invasive FoxM1 phosphorylated by PLK1 induces the polarization of tumor-associated macrophages to promote immune escape and metastasis, amplified by IFITM1.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Xu,Young-Joo Lee,Chang-Hyeon Kim,Ga-Hong Min,Yeo-Bin Kim,Jung-Won Park,Dae-Hoon Kim,Jung-Hyun Kim,Hyungshin Yim

Journal of personalized medicine 13: PubMed37763186

2023

Factors Affecting the Potential Efficacy of Intrauterine Platelet-Rich Plasma Infusion on Thin Endometrium in Women with Recurrent Implantation Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Pin-Yao Lin,Chun-I Lee,Yi-Chun Chen,En-Hui Cheng,Chun-Chia Huang,Chung-I Chen,Tsung-Hsien Lee,Yu-Jen Lee,Maw-Sheng Lee

In vivo (Athens, Greece) 37:2057-2069 PubMed37652524

2023

Effects of the Foam Massage Roller on VEGF-A and FGF-2 Blood Levels in Young Men.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Roslanowski,Aleksandra Partynska,Katarzyna Ratajczak-Wielgomas,Alicja Kmiecik,Jedrzej Grzegrzolka,Piotr Dziegiel,Adam Januszko,Dariusz Lenart,Waldemar Andrzejewski

Regenerative therapy 21:322-330 PubMed36110972

2022

MiR-590-3p affects the function of adipose-derived stem cells (ADSCs) on the survival of skin flaps by targeting VEGFA.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Yang,Xiancheng Wang,Yang Sun,Xiang Xiong,Xianxi Meng,Bairong Fang,Wenbo Li,Zhongjie Yi
View all publications
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