JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB277458

Human VEGFB ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human VEGFB ELISA Kit is a Sandwich ELISA for the measurement of Human VEGFB in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

VRF, VEGFB, Vascular endothelial growth factor B, VEGF-B, VEGF-related factor

1 Images
ELISA - Human VEGFB ELISA Kit (AB277458)
  • ELISA

Supplier Data

ELISA - Human VEGFB ELISA Kit (AB277458)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.4 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB270213

Human TIMP2 ELISA Kit

Recombinant|SimpleStep

ELISA - Human TIMP2 ELISA Kit (AB270213)

  • 5
  • 1
Assay Kits

AB315050

pan-HMR VEGFC ELISA Kit

SimpleStep

ELISA - pan-HMR VEGFC ELISA Kit (AB315050)

  • 0
  • 0
Assay Kits

AB65354

Superoxide Dismutase Activity Assay Kit (Colorimetric)

Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (AB65354)

  • 4
  • 2
Assay Kits

AB222510

Human VEGF ELISA Kit

SimpleStep

ELISA - Human VEGF ELISA Kit (AB222510)

  • 5
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human VEGFB ELISA Kit (ab277458) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGFB in serum (human VEGFB concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Human VEGFB coated on a 96-well plate. Standards and samples are pipetted into the wells and Human VEGFB present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human VEGFB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human VEGFB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "70 - 118 %", "average": "= 90.69" }, { "sample": "Plasma", "range": "69 - 149 %", "average": "= 104.8" }, { "sample": "Cell culture media", "range": "71 - 137 %", "average": "= 103.6" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277458-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277458-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277458-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human VEGFB coated Microplate", "number":"AB277458-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB277458-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277458-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin Concentrate", "number":"AB277458-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human VEGFB standard protein (Lyophilized)", "number":"AB277458-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277458-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human VEGFB Antibody", "number":"AB277458-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGFB also known as vascular endothelial growth factor B is a member of the VEGF family. It has a molecular mass of approximately 34-35 kDa. VEGFB interacts with its receptors to promote angiogenesis increasing blood supply to tissues. Expression of VEGFB occurs in the heart skeletal muscle and various endothelial cells. It also shows expression in tissues undergoing high metabolic activities. Besides its angiogenic role VEGFB exhibits participation in metabolic regulation.
Biological function summary

VEGFB supports angiogenesis by promoting endothelial cell growth and migration. It acts as a signal protein not forming part of a larger complex. VEGFB binds to VEGFR-1 (Flt-1) receptor which is involved in transmitting the growth-promoting signals. Its function extends to the regulation of fatty acid uptake into tissues highlighting its role in lipid metabolism and energy utilization.

Pathways

The signalling activities of VEGFB are significant in angiogenesis and metabolic pathways. VEGFB interacts closely with the VEGF-A pathway which is vital for forming new blood vessels. It also shares interactive roles with similar proteins such as VEGF-C and VEGF-D contributing to various angiogenic processes and cellular growth signalling systems.

Understanding VEGFB's role is important in conditions like cardiovascular disease and metabolic syndrome. Altered VEGFB signalling may link to heart diseases due to its regulatory function in maintaining heart vasculature. VEGFB has connections with VEGFR-1 and VEGF-A indicating its potential involvement in endothelial dysfunction and highlighting its significance in therapeutic strategies targeting such diseases.
style
left-column

Product protocols

style
left-column

Target data

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
See full target information VEGFB
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com