Human VEGFC ELISA Kit is a sandwich ELISA designed to quantify Human VEGFC with a sensitivity of 15 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 13.72 - 10000 pg/mL
View Alternative Names
Vascular endothelial growth factor C, VEGF-C, Flt4 ligand, Vascular endothelial growth factor-related protein, Flt4-L, VRP, VEGFC
- sELISA
Unknown
Sandwich ELISA - Human VEGFC ELISA Kit (AB100664)
Representative standard curve using ab100664
- sELISA
Unknown
Sandwich ELISA - Human VEGFC ELISA Kit (AB100664)
Representative standard curve using ab100664
Reactivity data
Product details
Human VEGFC ELISA Kit ab100664 is a sandwich ELISA to measure Human VEGFC in serum, plasma, cell culture supernatant with a sensitivity of 15 pg/ml.
How the assay works
This assay employs an antibody specific for Human VEGFC coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGFC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGFC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGFC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human VEGFC ELISA Kit ab100664 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
Optimization may be required with urine samples.
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
VEGFC contributes to the regulation of vascular and lymphatic endothelial cell functions. It is not known to be part of a larger protein complex but exerts its functions through interactions with receptors such as VEGFR-3. By promoting cell proliferation and migration VEGFC assists in the repair and regeneration of tissues. Its role in fluid balance is essential as it helps maintain tissue equilibrium and immune function.
Pathways
VEGFC is integral to the VEGF signaling and lymphatic vessel development pathways. Within these processes VEGFC works alongside other proteins such as VEGFA and VEGFB to modulate vascular permeability and vessel formation. The interaction between VEGFC and the VEGFR-3 receptor specifically mediates lymphatic endothelial cell activation and function linking it to vital physiological processes like inflammation and tissue repair.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (1)
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Experimental and therapeutic medicine 14:3253-3258 PubMed28966690
2017
Applications
Unspecified application
Species
Unspecified reactive species
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Product promise
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