AB246526

Human Vimentin ELISA Kit

Recombinant
SimpleStep
What is this?

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(2 Publications)

Human Vimentin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Vimentin with a sensitivity of 1.34 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Vimentin, VIM

3 Images

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)

sELISA

Supplier Data

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)

Example of human Vimentin standard curve in 1X Cell Extraction Buffer PTR.

The Vimentin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)

Interpolated concentrations of native Vimentin in human cell extracts at a 250 ng/mL extract load.

The concentrations of Vimentin were measured in duplicate and interpolated from the Vimentin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vimentin concentration was determined to be 65.2 ng/mL in A549 extracts.

sELISA

Supplier Data

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)

Representative image of Human Vimentin ELISA Kit (ab246526)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Vimentin Capture Antibody

- 10X Human Vimentin Detector Antibody

- Human Vimentin Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.34 ng/mL

Range

1.5625 - 100 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Vimentin ELISA Kit (ab246526) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Vimentin protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vimentin with 1.34 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

The standard is affinity purified.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "88 - 103 %", "average": "= 99" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab246526-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB246526-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246526-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB246526-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246526-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB246526-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB246526-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Vimentin Lyophilized Recombinant Protein", "number":"AB246526-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB246526-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Vimentin Capture Antibody", "number":"AB246526-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246526-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Vimentin Detector Antibody", "number":"AB246526-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB246526-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Vimentin, a class-III intermediate filament, is present in various non-epithelial cells, particularly mesenchymal cells, and connects either laterally or terminally to the nucleus, endoplasmic reticulum, and mitochondria. It is involved with LARP6 in stabilizing type I collagen mRNAs for CO1A1 and CO1A2. This supplementary information is collated from multiple sources and compiled automatically.

See full target information VIM

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Mucosal immunology 17:124-136 PubMed38007005

2023

Salivary IgA and vimentin differentiate in vitro SARS-CoV-2 infection: A study of 290 convalescent COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Ellis,Rosie Way,Miranda Nel,Alice Burleigh,Ivan Doykov,Japhette Kembou-Ringert,Maximillian Woodall,Tereza Masonou,Katie-Marie Case,Arturo Torres Ortez,Timothy D McHugh,Antonio Casal,Laura E McCoy,Sudaxshina Murdan,Robert E Hynds,Kimberly C Gilmour,Louis Grandjean,Mario Cortina-Borja,Wendy E Heywood,Kevin Mills,Claire M Smith

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:727-736 PubMed34118143

2021

Baicalein modulates the radiosensitivity of cervical cancer cells in vitro via miR-183 and the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Lei,Jingbin Shi,Yun Teng,Chenghui Song,Lijuan Zou,Fuxiu Ye,Haichen Zhang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Vimentin ELISA Kit

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)

Sandwich ELISA - Human Vimentin ELISA Kit (AB246526)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

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Product promise