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AB173190

Human Vimentin Profiling ELISA Kit

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(1 Publication)

Human Vimentin Profiling ELISA Kit is a ELISA for the measurement of Human Vimentin Profiling in Human in Cell/Tissue Extracts, Cell Samples samples.

View Alternative Names

Vimentin, VIM

3 Images
Sandwich ELISA - Human Vimentin Profiling ELISA Kit (AB173190)
  • sELISA

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Sandwich ELISA - Human Vimentin Profiling ELISA Kit (AB173190)

Typical standard curve generated when using the Vimentin Human Profiling ELISA kit.

Sandwich ELISA - Human Vimentin Profiling ELISA Kit (AB173190)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vimentin Profiling ELISA Kit (AB173190)

Experiment showing total Vimentin levels in various cell lines.

HeLa cell lysate, Jurkat cell lysate, SH SY5Y cell lysate, MCF7 cell lysate (not shown) and MDA-MB-231 cell lysate were tested using this kit. HeLa cell lysate shown as standard control sample, varying concentration other cell Lysates were analyzed within working range of the assay.

Sandwich ELISA - Human Vimentin Profiling ELISA Kit (AB173190)
  • sELISA

Supplier Data

Sandwich ELISA - Human Vimentin Profiling ELISA Kit (AB173190)

Vimentin protein expression level in the analyzed cell lysates.

Western blot of cell lysates (top panel) : 1) HeLa, 2) Jurkat, 3) Sy5y, 4) MCF7, 5) MDA-MB-231 (10 μg/lane) using primary antibodies : rabbit anti-vimentin (1/1000 dilution) and mouse anti-vimentin (ab8978, 1/1000 dilution); Secondary antibodies used were goat anti‑rabbit 680-RD (Red, 1/10,000) and goat anti‑mouse 800 (Green, 1/10,000). Blot was scanned using a LI­­‑COR® Odyssey® imager and overlay shows target specificity for total vimentin (yellow). Bar graph (bottom panel) represents relative mOD/min (600 nm) observed for 10 μg/mL cell lysate, except for MDA-MB-231 cell lysate which reports mOD/min (600 nm) for 1 μg/mL.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Suspension cells, Tissue Homogenate, Adherent cells

Reacts with

Human

Results type

Quantitative

Sensitivity

= 8 µg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Vimentin Human Profiling in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of total vimentin protein in Human samples.

The assay employs an antibody specific to Vimentin coated onto well plate strips. Samples are pipetted into the wells and Vimentin present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-Vimentin detector antibody is added. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted into the wells. The wells are again washed, a TMB development solution is added to the wells and blue color develops in proportion to the amount of bound Vimentin.

Vimentin is a type III intermediate filament (IF) protein that forms the cytoskeletal framework in the cytoplasm of eukaryotic cells. Other structural proteins such as actin and tubulin are highly conserved in different cell types, whereas IF proteins are expressed in a highly tissue specific manner. Due to vimentin being the major cytoskeletal protein in mesenchymal cells, it is used as a marker of mesenchymally-derived cells or cells undergoing epithelial-to-mesenchymal transition (EMT) during normal development and metastatic progression.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" } ]
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Recovery

[ { "sample": "Bovine Serum Albumin", "range": "83 - 84 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "71 - 87 %", "average": "= 80" }, { "sample": "Fetal Bovine Serum", "range": "81 - 93 %", "average": "= 89" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab173190-1x96Tests", "assetComponentDetails": [ { "size":"1 x 300 µg", "name":"HeLa Whole Cell RIPA Extract", "number":"AB173190-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"10X Blocking Buffer", "number":"AB173190-CMP07", "productcode":"" }, { "size":"1 x 40 mL", "name":"10X Wash Buffer", "number":"AB173190-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"10X HRP Label", "number":"AB173190-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Vimentin Microplate", "number":"AB173190-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"Extraction Buffer", "number":"AB173190-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"10X Vimentin Detector Antibody", "number":"AB173190-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"HRP Development Solution", "number":"AB173190-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
See full target information VIM
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 21: PubMed32526920

2020

Influence of Implant Material and Surface on Mode and Strength of Cell/Matrix Attachment of Human Adipose Derived Stromal Cell.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Jung,Lauren Bohner,Marcel Hanisch,Johannes Kleinheinz,Sonja Sielker
View all publications
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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