Human Vimentin Profiling ELISA Kit is a Quantitative ELISA kit for the measurement of Human Vimentin Profiling in Human in Tissue Lysate, Suspension cells, Tissue Homogenate, Adherent cells samples.
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- International journal of molecular sciences 21:2020Influence of Implant Material and Surface on Mode and Strength of Cell/Matrix Attachment of Human Adipose Derived Stromal Cell.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusanne Jung et. al.PubMed 32526920
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Suspension cells, Tissue Homogenate, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Human
- Sensitivity
- = 8 µg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Vimentin, a class-III intermediate filament, is present in various non-epithelial cells, particularly mesenchymal cells, and connects either laterally or terminally to the nucleus, endoplasmic reticulum, and mitochondria. It is involved with LARP6 in stabilizing type I collagen mRNAs for CO1A1 and CO1A2. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
Vimentin, VIM
What's included?
Recommended products
Human Vimentin Profiling ELISA Kit is a Quantitative ELISA kit for the measurement of Human Vimentin Profiling in Human in Tissue Lysate, Suspension cells, Tissue Homogenate, Adherent cells samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Suspension cells, Tissue Homogenate, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Human
- Assay Platform
- Microplate
- Sensitivity
- = 8 µg/mL
- General recovery
- > 80%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 3 | C.V. 7.5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 3 | C.V. 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Bovine Serum Albumin | Average % = 89 | Range 83 - 84 % |
Sample type Cell culture media | Average % = 80 | Range 71 - 87 % |
Sample type Fetal Bovine Serum | Average % = 89 | Range 81 - 93 % |
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Vimentin Human Profiling in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of total vimentin protein in Human samples.
The assay employs an antibody specific to Vimentin coated onto well plate strips. Samples are pipetted into the wells and Vimentin present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-Vimentin detector antibody is added. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted into the wells. The wells are again washed, a TMB development solution is added to the wells and blue color develops in proportion to the amount of bound Vimentin.
Vimentin is a type III intermediate filament (IF) protein that forms the cytoskeletal framework in the cytoplasm of eukaryotic cells. Other structural proteins such as actin and tubulin are highly conserved in different cell types, whereas IF proteins are expressed in a highly tissue specific manner. Due to vimentin being the major cytoskeletal protein in mesenchymal cells, it is used as a marker of mesenchymally-derived cells or cells undergoing epithelial-to-mesenchymal transition (EMT) during normal development and metastatic progression.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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3 product images
Sandwich ELISA - Human Vimentin Profiling ELISA Kit (ab173190)
Typical standard curve generated when using the Vimentin Human Profiling ELISA kit.
Sandwich ELISA - Human Vimentin Profiling ELISA Kit (ab173190)
Experiment showing total Vimentin levels in various cell lines.
HeLa cell lysate, Jurkat cell lysate, SH SY5Y cell lysate, MCF7 cell lysate (not shown) and MDA-MB-231 cell lysate were tested using this kit. HeLa cell lysate shown as standard control sample, varying concentration other cell Lysates were analyzed within working range of the assay.
Sandwich ELISA - Human Vimentin Profiling ELISA Kit (ab173190)
Vimentin protein expression level in the analyzed cell lysates.
Western blot of cell lysates (top panel): 1) HeLa, 2) Jurkat, 3) Sy5y, 4) MCF7, 5) MDA-MB-231 (10 μg/lane) using primary antibodies: rabbit anti-vimentin (1/1000 dilution) and mouse anti-vimentin (Anti-Vimentin antibody [RV202] - Cytoskeleton Marker ab8978, 1/1000 dilution); Secondary antibodies used were goat anti‑rabbit 680-RD (Red, 1/10,000) and goat anti‑mouse 800 (Green, 1/10,000). Blot was scanned using a LI‑COR® Odyssey® imager and overlay shows target specificity for total vimentin (yellow). Bar graph (bottom panel) represents relative mOD/min (600 nm) observed for 10 μg/mL cell lysate, except for MDA-MB-231 cell lysate which reports mOD/min (600 nm) for 1 μg/mL.
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