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AB264623

Human Visfatin ELISA Kit

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(4 Publications)

Human Visfatin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Visfatin with a sensitivity of 80 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PBEF, PBEF1, NAMPT, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Visfatin, Pre-B cell-enhancing factor

3 Images
Sandwich ELISA - Human Visfatin ELISA Kit (AB264623)
  • sELISA

Supplier Data

Sandwich ELISA - Human Visfatin ELISA Kit (AB264623)

Example of human Visfatin standard curve in 1X Cell Extraction Buffer PTR.

The Visfatin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Visfatin ELISA Kit (AB264623)
  • sELISA

Supplier Data

Sandwich ELISA - Human Visfatin ELISA Kit (AB264623)

Representative image of Human Visfatin ELISA Kit (ab264623)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Visfatin ELISA Kit Capture Antibody

- 10X Human Visfatin Detector Antibody

- 10X Human Visfatin ELISA Kit Lyophilised Purified Protein (2 vials)

- Human Visfatin ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human Visfatin ELISA Kit (AB264623)
  • sELISA

Supplier Data

Sandwich ELISA - Human Visfatin ELISA Kit (AB264623)

Interpolated concentrations of native Visfatin in human liver tissue extract and K562 cell extract based on a 15 μg/mL and 50 μg/mL extract load.

The concentrations of Visfatin were measured in duplicate and interpolated from the Visfatin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Visfatin concentration was determined to be 7,060.81 pg/mL in liver tissue extract and 10,559.80 pg/mL in K562 cell extract.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 80 pg/mL

Range

234.38 - 15000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Visfatin ELISA Kit (ab264623) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Visfatin protein in cell lysate and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Visfatin with 80 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Visfatin, also known as nicotinamide phosphoribosyltransferase, is the key enzyme in the salvage pathway by which the cell converts nicotinamide to nicotinamide mononucleotide. Along with its enzymatic role, Visfatin is thought to play a pivotal role in B-cell maturation and cell signaling. This protein is highly conserved throughout mammals, subsequently, this kit cross-reacts with both mouse and rat.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "90 - 96 %", "average": "= 93" }, { "sample": "Cell Lysate", "range": "97 - 114 %", "average": "= 104" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264623-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264623-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264623-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264623-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB264623-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264623-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264623-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264623-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Visfatin Capture Antibody", "number":"AB264623-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Visfatin Lyophilized Recombinant Protein", "number":"AB264623-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB264623-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB264623-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Visfatin Detector Antibody", "number":"AB264623-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component : CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).
See full target information NAMPT
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Egyptian heart journal : (EHJ) : official bulletin of the Egyptian Society of Cardiology 75:63 PubMed37466800

2023

Pre-procedural high serum visfatin and tumor necrosis factor-α might predict recurrent atrial fibrillation after catheter ablation.

Applications

Unspecified application

Species

Unspecified reactive species

Amr AlKassas,Mohamed Fouda,Gaetano Fassini,Mohamed Sanhoury

Endocrine 80:448-458 PubMed36658296

2023

Visfatin induces ovarian cancer resistance to anoikis by regulating mitochondrial activity.

Applications

Unspecified application

Species

Unspecified reactive species

Justyna Gogola-Mruk,Wacław Tworzydło,Kinga Krawczyk,Weronika Marynowicz,Anna Ptak

Biomarker insights 17:11772719221137608 PubMed36386121

2022

Evaluation of Genetic Polymorphisms of the Antioxidant Enzymes and Biomarkers of Oxidative Stress in Preterm Neonates With Respiratory Distress Syndrome Receiving External Surfactant.

Applications

Unspecified application

Species

Unspecified reactive species

Kannan Sridharan,Mona Al Jufairi,Aamal AbdulGhani Mahdi Hejab,Abdulraoof Al Madhoob,Reem Al Marzooq,Safa Taha,Muna Jaber Mulla Aljishi,Ameera Abdulhadi,Eman Al Ansari,Masooma Abdulla Ali,Maryam Ali Ahmed Naser,Ola Al Segai,Kevin Dunne

Journal of personalized medicine 12: PubMed35629157

2022

Differential Association of Selected Adipocytokines, Adiponectin, Leptin, Resistin, Visfatin and Chemerin, with the Pathogenesis and Progression of Type 2 Diabetes Mellitus (T2DM) in the Asir Region of Saudi Arabia: A Case Control Study.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Muzaffar Mir,Rashid Mir,Mushabab Ayed Abdullah Alghamdi,Javed Iqbal Wani,Zia Ul Sabah,Mohammed Jeelani,Vijaya Marakala,Shahzada Khalid Sohail,Mohamed O'haj,Muffarah Hamid Alharthi,Mohannad Mohammad S Alamri
View all publications
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websiteProtocolBooklet
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Product promise

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